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Externally-applied dressing and preparation method and application thereof

A technology of dressing and raw material solution, applied in the medical field, can solve the problems of expensive exogenous growth factors, complex pathological mechanism, and loss of active ingredients in the gel

Active Publication Date: 2016-10-12
上海市伤骨科研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex pathological mechanism of refractory skin wounds such as diabetes mellitus, only adding one or two growth factors cannot accelerate wound healing very effectively; exogenous growth factors are expensive
Recombinant PDGF-BB still has the following shortcomings: 1. This drug loading and treatment method still belongs to the category of traditional topical medication, and patient activities, covering dressings (such as gauze, etc.) and local friction can cause the active ingredients of the gel to be lost , reducing the curative effect; 2. The drug contained in the gel cannot be released slowly, and the drug release effect cannot be accurately calculated due to the loss and loss of the gel

Method used

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  • Externally-applied dressing and preparation method and application thereof
  • Externally-applied dressing and preparation method and application thereof
  • Externally-applied dressing and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: the preparation of dressing

[0059] In this example, an 8% by weight solution of medical grade polyvinyl alcohol in deionized water was prepared. Independently medical chitosan is dissolved in the aqueous solution of 1% by weight of acetic acid with the concentration of 2% by weight, adds the deferoxamine solid powder of pre-calculated amount in this chitosan solution, forms chitosan solution, described Feroxamine was added in such an amount that the deferoxamine content in the final dressing was 1% by weight of the total dressing solids. The polyvinyl alcohol solution prepared above and the chitosan solution were mixed in a weight ratio of 4:6 and placed in a syringe pump. Using the electrospinning machine shown in Figure 1 of the present invention, a 0.6mm stainless steel needle is selected as the spinning nozzle and connected to a high-voltage positive electrode, and a rotating receiving plate is set at a distance of 8 cm from the needle. The voltage...

Embodiment 2

[0062] Example 2: Control dressing (dressing without deferoxamine)

[0063] In this example, the preparation steps of Example 1 were repeated to form a dressing, but the difference was that deferoxamine was not used in the raw material, and the prepared dressing was recorded as a control dressing.

Embodiment 3

[0064] Example 3: In this example, the performance of VEGF protein expression was tested using the dressings of the present invention prepared in Example 1 above with different contents of deferoxamine.

[0065] sample culture

[0066] Dressings with DFO content of 0.8, 0.9, 1.0% by weight were cut into 15x15 mm 2 The size of the square is preset at the bottom of the 6-well plate, according to 2x10 5 Seed skin fibroblasts at cell density, place at 37°C, 5% CO 2 Incubate in the incubator and replace the medium every other day. The protein in the cells was extracted on the third day of the growth of the cultured cells, with at least 3 samples in each group, and repeated 3 times.

[0067] protein extraction

[0068] The steps of protein extraction are as follows:

[0069] (1) 100 μl RIPA (Beyotime, China) and 1 μl protease inhibitor PMSF (Beyotime, China)) were added to each well of a 6-well plate, and placed on ice.

[0070] (2) Scrape the cells carefully with a cell s...

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Abstract

The invention provides an externally-applied dressing and a preparation method and an application thereof. The externally-applied dressing is formed by a composite fiber, wherein the composite fiber comprises A, a synthetic polymer, B, a natural polymer, and C, desferrioxamine. The preparation method of the externally-applied dressing comprises the steps of mixing a solution containing the synthetic polymer, a solution containing the natural polymer, and desferrioxamine to form a spinning raw material solution, and then carrying out spinning treatment of the spinning raw material solution. The invention also provides the application of the externally-applied dressing in preparation of products for treatment of traumatic skin injury.

Description

technical field [0001] The present invention generally relates to the medical field, and more specifically, the present invention relates to an external dressing capable of promoting wound repair. The dressing can not only keep the wound clean, but also effectively promote angiogenesis and accelerate the healing process. The invention also provides a preparation method of the external dressing and its application in the treatment of skin trauma. Background technique [0002] The key steps in skin wound healing are the growth of new blood vessels into the wound and the formation of granulation tissue. For some common traumatic wounds, wrapping and protecting them with dressings and taking oral or external drug treatment can make the wound heal. For some chronic and difficult-to-heal wounds, such as foot skin wounds of diabetic patients, skin bedsore wounds caused by long-term bedridden patients, etc., it is difficult to achieve the purpose of wound healing simply by changing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/24A61L15/28A61L15/20A61L15/44A61L15/42A61L15/64
CPCA61L15/20A61L15/24A61L15/28A61L15/42A61L15/44A61L15/64A61L2300/204A61L2300/412C08L29/04C08L5/08
Inventor 邓廉夫陈皓齐进崔文国
Owner 上海市伤骨科研究所
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