Alcaligenes and its application to the treatment of chromium-containing wastewater

A technology for wastewater treatment and alkali-producing bacteria, which is used in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., and can solve the problems of low reducing ability, low hexavalent chromium resistance, and long reduction time. problem, to reduce toxicity and promote transformation

Active Publication Date: 2019-05-14
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these existing microorganisms have little resistance to hexavalent chromium, especially the reduction ability to hexavalent chromium concentration exceeding 200mg / L is low, or the time required for reduction is longer, and there is still a certain distance from the application of high-concentration chromium pollution control in industry. distance

Method used

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  • Alcaligenes and its application to the treatment of chromium-containing wastewater
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  • Alcaligenes and its application to the treatment of chromium-containing wastewater

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The screening of embodiment 1 alcaligenes, reducing ability detection and strain identification

[0023] 1) Screening:

[0024] Chromium-containing wastewater was collected from the vicinity of the Yiwu chrome mine in Henan Province, stored at 4°C, and transported to the laboratory as screening materials for target bacteria.

[0025] Prepare high-concentration liquid chromium-containing medium and solid chromium-containing medium respectively according to the following formula, and the liquid chromium-containing medium is as follows: tryptone 10g, yeast extract 5g, NaCl 10g, potassium dichromate (K 2 Cr 2 o 7 ) 2.46g Shake the container until the solute is dissolved, adjust the pH to 7.0 with 5mol / L NaOH, make up to 1L with deionized water, and steam sterilize at 121°C for 20min under high pressure. The solid chromium-containing medium is the same as the liquid chromium-containing medium, just add 15g of agar powder.

[0026] Add 3mL of chromium-containing waste wat...

Embodiment 2

[0034] The biological characteristic of embodiment 2 Alcaligenes sp.GTM2:

[0035] Gram stain was negative, with motility. The strain Alcaligenes sp.GTM2 presents a diameter of 1.0-1.5mm on a solid medium plate, round, milky white opaque, sticky, and neatly bordered colonies, such as figure 1 shown. The results observed under the scanning electron microscope are as follows figure 2 As shown in (A), the image shows that the cells of Alcaligenes sp.GTM2 are about 2-3 μm long and 0.5-1 μm wide, and do not produce spores; after being treated with 1000 mg / L Cr(VI) for 48 hours, the surface of Alcaligenes sp.GTM2 appears uneven, such as figure 2 (B) shown.

[0036] The preservation method of the bacterial strain is as follows: 50% glycerin is mixed with an equal volume of bacterial strain and stored in a -80° C. refrigerator.

Embodiment 3

[0037] Example 3 Alcaligenes sp.GTM2 bacterial strain reduction experiment to high concentration chromium-containing wastewater (1000mg / L hexavalent chromium)

[0038] Insert Alcaligenes sp.GTM2 into 200mL LB culture medium (LB formula: tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; pH=8.0; sterilize at 121°C for 20min), after night activation Afterwards, the number of viable bacteria of Alcaligenes sp.GTM2 can reach more than 1.0×108CFU / ml, and the inoculum amount of 1 / 100 (v / v) is transferred to 500mL of fresh chromium containing 1000mg / L and chromium-free Culture in sterilized LB medium, 38°C, 180rpm shaker, take samples every 8 hours, count with a hemocytometer, draw the growth of GTM2 under the culture conditions of potassium dichromate concentration 1000mg / L and no potassium dichromate curves, such as image 3 shown. Simultaneously use ferrous ammonium sulfate titration and adopt diphenylcarbazide spectrophotometry to detect the change of total chromium and...

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Abstract

The invention discloses an alcaligenes and its application to the treatment of chromium-containing wastewater. The strain is Alcaligenes sp.GTM2, and the strain is preserved in the General Microorganism Center of China Microbial Strain Preservation Management Committee, and the preservation place is Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1, Courtyard 1, Beichen West Road, Chaoyang District. The preservation registration number is CGMCC No. 12347, and the preservation date is April 15, 2016. Alcaligenes GTM2 provided by the present invention has a reduction effect on hexavalent chromium: it is reduced from 1000mg / L to 110mg / L within 24h, and the reduction rate is 89%; Direct dosing of 1% Alcaligenes sp.GTM2 bacteria has a strong reduction ability, and the reduction rate is about 28-56%. Therefore, it can be used for the restoration of chromium-polluted water bodies, which can promote the conversion of available chromium to unusable chromium, reduce Chromium toxicity.

Description

technical field [0001] The invention relates to the technical field of environmental microbial restoration, in particular to an Alcaligenes sp. and its application to the treatment of chromium-containing wastewater. Background technique [0002] With the rapid development of the global economy, heavy metal pollution has become one of the more and more important environmental issues. Although chromium is an essential trace element for the human body, excessive hexavalent chromium is highly toxic because hexavalent chromium can pass through prokaryotic and eukaryotic cell membranes to cause oxidative cell damage and cause teratogenic and carcinogenic diseases. [0003] Chromium salt series products are important basic raw materials for chemical industry-light industry-advanced alloy materials, and are widely used in tanning, electroplating, alloys, pigments, printing and dyeing, offset printing and agriculture. The annual output of chromium salt in my country has exceeded 160...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/05C02F101/22
CPCC02F3/34C02F2101/22C12N1/205C12R2001/05
Inventor 庄绪亮徐圣君高天明金德才白志辉庄国强
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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