White rot fungus biological microsphere and preparation method thereof

A kind of white rot fungus biology and white rot fungus technology, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of low reuse rate, harsh growth environment requirements, hindering industrial application, etc., and achieve repeated The effect of high utilization rate, reduction of secondary pollution and avoidance of negative effects

Active Publication Date: 2016-10-12
山东济清科技服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the harsh requirements on the growth environment of white rot fungi, the dependence on nutrient substrates and the particularity of their physiological metabolism, the reuse rate is low, which hinders its industrial application.

Method used

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  • White rot fungus biological microsphere and preparation method thereof
  • White rot fungus biological microsphere and preparation method thereof
  • White rot fungus biological microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) Preparation of plant straw powder fiber:

[0036] Use a straw crusher to crush the plant straw, and then pass it through a 200-mesh screen to obtain plant straw powder fibers of about 75 μm;

[0037] 2) Preparation of plant straw powder fiber and white rot fungus co-substrate:

[0038] White rot fungus (Phanerochaete chrysosporium) was cultured on liquid medium for one week, filtered, collected white rot fungus mycelium, placed in water, broken up by glass beads and shaken, adding plant straw powder fiber, The mass ratio of mycelium to plant straw powder fiber is 10:1, mix well, culture the mixture in liquid culture medium for 2 days, use fine gauze to filter and remove the culture medium, and obtain the co-substrate of plant straw powder fiber and white rot fungus body; liquid medium components are: glucose 20g / L, KH 2 PO 4 2g / L, MgSO 4 0.25g / L, CaCl 2 0.1g / L, MnSO 4 5mg / L, VB 1 5mg / L, ammonium tartrate 0.2g / L, trace elements 150mL / L;

[0039] 3) Prepar...

Embodiment 2

[0044] 1) Preparation of plant straw powder fiber:

[0045] Use a straw crusher to crush the plant straw, and then pass it through a 200-mesh screen to obtain plant straw powder fibers of about 75 μm;

[0046] 2) Preparation of plant straw powder fiber and white rot fungus co-substrate:

[0047] White rot fungus (trametes versicolor) in solid medium (components: potato extract 200g / L, glucose 20g / L, agar 20g / L, KH 2 PO 4 3g / L, MgSO 4 1.5g / L) for one week, use an inoculation loop to gently scrape the surface of the solid medium to collect white rot fungus spores, put them in water, break up the spores with glass beads and shake them well, add plant straw powder fibers, spores and plant straw powder The fiber mass ratio is 1:10, mix evenly, and put the mixed body in the liquid medium (the components are: glucose 20g / L, KH 2 PO 4 2g / L, MgSO 4 0.25g / L, CaCl 2 0.1g / L, MnSO 4 5mg / L, VB 1 5mg / L, ammonium tartrate 0.2g / L, trace elements 150mL / L) for 2 days, filter the ...

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Abstract

The invention relates to a white rot fungus biological microsphere and a preparation method thereof. The preparation method comprises the following steps: preparing a plant straw powder and white rot fungus co-substrate body, preparing a white rot fungus biological microsphere, and embedding the plant straw powder and white rot fungus co-substrate body in the white rot fungus biological microsphere. The biological microsphere prepared in the invention solves the problems of low wastewater treatment stability of white rot fungi and consumption of a nutrient matrix source by immobilized white rot fungi, can be used to great wastewater generated in the leather making industry, the fur industry and the textile printing and dyeing industry, and has the advantages of no need of an applied nutrient source, strong environment adaptability, strong degradation ability, non-toxicity and no hidden safety troubles.

Description

technical field [0001] The invention belongs to the technical field of applied microorganisms and water treatment, and relates to an adsorption degradation material for treating dyestuffs, tannin extracts, and chromium-containing wastewater, in particular to a white-rot fungus biological microsphere and a preparation method. Background technique [0002] The retanning and dyeing section of the tanning industry uses a large amount of retanning agents and dyes and other refractory organic compounds. Most of the vegetable tanning agents, synthetic tanning agents and dyes are refractory aromatic organic compounds. Biological treatment under anaerobic conditions is difficult to solve the pollution problem of refractory aromatic compounds. Research on effective treatment methods of refractory organic pollutants has become an important topic in the treatment of tannery wastewater. White rot fungus has a unique degradation ability, has great development prospects in the field of en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/08C02F3/34C12R1/645
CPCC02F3/347C12N11/08C12N11/10
Inventor 李彦春徐旭东祝德义
Owner 山东济清科技服务有限公司
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