Aminotic cell culture medium for high-density cell culture system

Abstract

The present invention relates to an aminotic cell culture medium for a high-density cell culture system. The aminotic cell culture medium comprises a base culture medium and added components, wherein the base culture medium is a F12 culture medium, and the added components comprise fetal bovine serum with a concentration of 80-120 ml / L, human recombinant insulin with a concentration of 24-30 mg / L, human recombinant epidermal growth factor with a concentration of 20-25 [mu]g / L, human recombinant basic fibroblast growth factor with a concentration of 35-45 [mu]g / L, human albumin with a concentration of 10-15 g / L, transferring with a concentration of 4-16 mg / L, Hydrocortisone with a concentration of 0.2-1 mg / L, Testosterone with a concentration of 0.2-1 mg / L, progesterone with a concentration of 0.2-1 mg / L, vitamin E with a concentration of 1-5 mg / L, L-glutamine with a concentration of 12-20 g / L, HEPES with a concentration of 5-10 g / L, and a block polyether F-68 with a concentration of 0.5-2 g / L, wherein the concentrations of each added component adopt the total volume of the aminotic cell culture medium as the reference. Compared with the aminotic cell culture medium in the prior art, the aminotic cell culture medium of the present invention has advantages of rapid and large-scale aminotic cell proliferation, good proliferation effect and short culture time, and is particularly suitable for the high-density cell culture system of the aminotic cells.

Description

technical field [0001] The invention relates to an amniotic fluid cell culture medium suitable for a high-density cell culture system, belonging to the technical field of cell culture. Background technique [0002] Prenatal diagnosis is an important part of eugenics. It is established on the basis of genetic counseling by testing and diagnosing the developmental status of the embryo or fetus and whether it is suffering from diseases before birth. Prenatal diagnosis currently mainly includes early villous tissue extraction, amniotic fluid and umbilical cord blood in the second trimester, and separation of fetal cells from maternal peripheral blood. Among them, amniotic fluid cell detection in the second trimester is still the most important diagnostic method. [0003] Regarding the detection of amniotic fluid cells in the second trimester, the amniotic fluid samples of pregnant women are extracted through amniocentesis, the amniotic fluid cells are cultured, or the DNA of th...

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Problems solved by technology

[0006] However, most of the traditional amniotic fluid culture media in the prior art are suitable for medium-scale cell culture systems, and generally cannot be suitable for large-scale, high-density cell culture; on the other hand, the existing serum-free medium It is only suitable for two-dimensional cell culture systems such as culture plates, culture dishes, and culture bottles, and cannot be applied to three-dimensional culture systems such as high-density cell culture tanks