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Porcine reproductive and respiratory syndrome virus liposome diluent lyophilized product and preparing method thereof

A technology for respiratory syndrome and freeze-dried products, which is applied in the field of freeze-dried products of porcine reproductive and respiratory syndrome virus liposome dilution and its preparation, and can solve the problems of low virus content, large differences between batches, and differences in semi-finished products. , to achieve the effect of enhancing immunogenicity, increasing virus content, and reducing batch-to-batch variation

Inactive Publication Date: 2016-11-09
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] One of the most important indicators for the quality assessment of veterinary biological products is the batch-to-batch stability. However, it is difficult to control the batch-to-batch stability of semi-finished products in the actual production process. The main reason is that the proportion of live organisms inoculated is uncertain , that is to say, the instability of the inoculation amount leads to large differences in the production of semi-finished products
The purpose of the present invention is to solve the problems of large batch-to-batch differences and low virus content during the propagation of porcine reproductive and respiratory syndrome virus

Method used

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  • Porcine reproductive and respiratory syndrome virus liposome diluent lyophilized product and preparing method thereof
  • Porcine reproductive and respiratory syndrome virus liposome diluent lyophilized product and preparing method thereof
  • Porcine reproductive and respiratory syndrome virus liposome diluent lyophilized product and preparing method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of PBS solution

[0020] Take 6-10g of sodium chloride, 0.05-0.5g of potassium chloride, 1-1.2g of disodium hydrogen phosphate, 0.05-0.5g of potassium dihydrogen phosphate, 0.05-0.2g of calcium chloride, 0.05g of magnesium chloride containing 6 crystal water -0.2g;

[0021] After mixing the above ingredients, dissolve them in 1000ml of double distilled water, and sterilize under high pressure at 116°C for 30 minutes.

Embodiment 2

[0022] Embodiment 2: the preparation of liposome

[0023] Weigh soybean lecithin 6g, cholesterol 2g, dissolve with 500ml chloroform, and make it mix uniformly, and remove chloroform in the solution by decompression method, prepare liposome membrane solution; And potassium citrate buffer solution 200ml, this buffer solution is added in the liposome membrane solution, is prepared into liposome with homogenizer after dehydration.

Embodiment 3

[0024] Embodiment 3: the preparation of liposome

[0025] Weigh 4g of soybean lecithin and 4g of cholesterol, dissolve them with 500ml of chloroform, and mix them uniformly, and remove the chloroform in the solution by decompression to prepare a liposome membrane solution; take citrate with a pH value of 7.0 to 8.0 And potassium citrate buffer solution 200ml, this buffer solution is added in the liposome membrane solution, is prepared into liposome with homogenizer after dehydration.

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Abstract

The invention provides a porcine reproductive and respiratory syndrome virus liposome diluent lyophilized product and a preparing method thereof, and belongs to the technical field of animal biological products. 1000 ml of a PBS solution contains 2-8 g of astragaloside III, 5-10 g of aloe polysaccharide, 2-6 g of curcumin, 10-15 g of lipidosome, 10-15 g of glucose and 10-15 g of mannitol. The prepared liposome diluent lyophilized product can reduce difference between semi-finished product batches and can promote propagation of porcine reproductive and respiratory syndrome viruses, increase virus content and enhance immunogenicity of vaccines.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, in particular to a freeze-dried product of porcine reproductive and respiratory syndrome virus liposome dilution and a preparation method thereof. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease of pigs caused by porcine reproductive and respiratory syndrome virus (PRRSV). Pigs of different ages, breeds and sexes can be infected , the disease is a highly pathogenic disease characterized by reproductive impairment in sows, respiratory symptoms in piglets, immunosuppression, high infection rate, and high mortality. The disease has caused serious economic losses to the global swine industry. In order to effectively control the disease, a variety of vaccines for preventing PRRS have been developed at home and abroad. [0003] Liposome is a phospholipid bilayer structure carrier with similar properties to biologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K9/127A61K47/36A61K47/28A61K47/26A61K47/10A61K39/12A61P31/14
CPCA61K9/19A61K9/127A61K39/12A61K47/10A61K47/26A61K47/28A61K47/36C12N2770/10034
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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