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Preparation method of human milk fat substitute

A technology for replacing fat and lipase, which is applied in the field of human milk replacement fat preparation, and can solve the problems of increasing deacidification cost, OPO content and influence on purity, etc.

Active Publication Date: 2016-11-16
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A big disadvantage of this method is that a large amount of free fatty acids will be generated, which will increase the cost of subsequent deacidification, and may affect the content and purity of OPO in the product

Method used

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  • Preparation method of human milk fat substitute
  • Preparation method of human milk fat substitute
  • Preparation method of human milk fat substitute

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Wash titanium dioxide with water, the electron microscope picture of titanium dioxide is as follows figure 1 As shown, an ordered porous structure can be observed at a resolution of 300 nm, which can provide sites for enzyme protein loading. The mesoporous titanium dioxide material was pre-treated in an oven at 120°C for 12 hours, and 1g of the treated TiO was taken 2 Granules, add dichloromethane with a mass-volume ratio of 1:10, then add 1-aminobutylphosphonic acid with a mass ratio of 3:1, stir at room temperature for 24 hours, filter with suction, and wash with acetone aqueous solution (volume ratio 1:1) , dried in an oven at 100°C for 8h. Obtained TiO 2 The material is recorded as 1-aminobutylphosphonic acid—TiO 2 .

[0039] like figure 2 As shown, the mesoporous TiO before modification 2 The particles have five obvious diffraction peaks at 2θ=25.3, 37.8, 48.1, 53.9 and 55.1, corresponding to (101), (004), (200), (105) and (211) crystals of anatase crystal f...

Embodiment 2

[0042] Take 1.25ml of Novozymes Cheese Flavor Lipase 20000L, dilute to 25ml with pH=7 phosphate buffer, take two 50ml centrifuge tubes, add 10ml of diluted enzyme solution, 0.1g of pure titanium dioxide and 1-aminobutylphosphonic acid respectively The modified titanium dioxide was oscillated overnight in a constant temperature oscillating reactor at 250r / min and 25°C. Then the centrifuge tube was centrifuged for 10 min at 8000 r / min. Finally, the supernatant was removed, and the product was dried in an oven at 60° C. to obtain the immobilized enzyme.

[0043] Figure 5 TiO 2 and 1-aminobutylphosphonic acid—TiO 2 Comparison chart of activity and specific activity of immobilized cheese essence lipase. The cheese essence lipase used in the experiment is derived from Aspergillus oryzae (Aspergillus oryzae), and is produced by submerged fermentation of genetically modified microorganisms. The enzyme protein itself is not genetically modified, but is isolated and purified from ...

Embodiment 3

[0045] Take 0.1g of Rhizopus oryzae lipase, adjust the volume to 100ml with pH=7 phosphate buffer, take two 50ml centrifuge tubes, add 10ml of diluted enzyme solution and 0.1g of pure titanium dioxide and 1-aminobutyl phosphonic acid modified Titanium dioxide was shaken overnight in a constant temperature shaking reactor under the conditions of 250r / min and 25°C. Then the centrifuge tube was centrifuged for 10 min at 8000 r / min. Finally, the supernatant was removed, and the product was dried in an oven at 60° C. to obtain the immobilized enzyme.

[0046] like Image 6 shown, with TiO 2 Compared to 1-aminobutylphosphonic acid—TiO 2 The enzyme loading of immobilized Rhizopus oryzae lipase decreased, and the unmodified TiO 2 The loading capacity is 8.3mg / g, and after modification, 1-aminobutylphosphonic acid—TiO 2 The loading amount is 6.51mg / g, the specific activity of the immobilized enzyme is calculated according to the enzyme activity and the enzyme loading amount, and t...

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Abstract

The invention discloses a preparation method of a human milk fat substitute. The method includes the following steps of 1, reaction, wherein palm stearin and vegetable oil are mixed and molten to obtain mixed oil, oleic acid or nonglyceride oleate or palmitic acid or nonglyceride palmitate is added and stirred to be mixed, and lipase immobilized on a mesoporous titania nanocomposite is added to catalyze transesterification; 2, extraction, wherein after transesterification is finished, the catalyst in the reaction mixture is removed through centrifugation, deacidification is carried out with the molecular distillation method to make the acid value of the end product not larger than 3.0 mg KOH g-1, and extract is obtained and used in the next step of processing; 3, mixing, wherein the extract obtained in the second step is mixed with one or more of sunflower seed oil, high-oleic-acid sunflower seed oil, rapeseed oil and linseed oil in the mass ratio of 1:(4-1), and the human milk fat substitute is obtained. The process is simple and controllable, and the similarity between the product and human milk fat can be easily improved.

Description

technical field [0001] The invention belongs to the technical field of food for infants and young children, and in particular relates to a preparation method of human milk substitute fat. Background technique [0002] As the natural food of choice for babies, breast milk is the main source of life energy for babies. Breast milk substitutes also play an important role in the health of infants and young children as backup nutrients. According to literature reports, the type, content and distribution of fatty acids in them have an important impact on the growth and development of infants and young children. Studies have shown that currently, most infant formula milk powder manufacturers on the market use blended vegetable oils to imitate the unique lipid composition of breast milk, but the lipid structure of this formula is significantly different from that of breast milk, and palmitic acid is mostly bound in The sn-1 and sn-3 positions of the glycerol backbone in the triglyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23D9/04
CPCA23D9/04
Inventor 应汉杰王振富庄伟吴菁岚周精卫陈勇陈晓春朱晨杰柳东牛欢青
Owner NANJING UNIV OF TECH
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