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Cordyceps sinensis fluid medium and preparation method thereof

A liquid culture medium, the technology of Cordyceps sinensis, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor quality of Cordyceps sinensis, differences in mycelium properties, low nutrient content, etc., and achieve excellent technical results Effect

Active Publication Date: 2016-11-23
青海珠峰冬虫夏草工程技术研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, many individuals and units in China have researched and disclosed Cordyceps sinensis culture medium and fermentation production methods. However, these technologies mostly use common culture medium for microbial fermentation, basically using eggs, milk, and potatoes as fermentation food sources. The selection of medium components, although all According to the fermentation principle of microbiology, the bacteria can grow, but the different food sources will inevitably lead to differences in the properties of the mycelium. The produced Cordyceps sinensis fermentation products are different from natural Cordyceps sinensis in chemical composition and active substances. Therefore, the existing The Cordyceps sinensis cultivated in the technology generally has the problems of poor quality and low nutrient content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Raw materials are weighed according to the following percentages by weight: 10% of Jerusalem artichoke tubers, 5% of potatoes, 5% of beef extract, 6% of fish meal, 0.08% of vitamin B1, 0.06% of riboflavin, 0.05% of folic acid, 0.1% of magnesium sulfate, and 0.1% of sodium chloride %, the balance of water;

[0022] (1) the Jerusalem artichoke tuber is pulverized and crossed a 100-mesh sieve to obtain the Jerusalem artichoke powder;

[0023] (2) Add Jerusalem artichoke powder, potato, beef extract, fish meal, vitamin B1, riboflavin, folic acid, magnesium sulfate and sodium chloride obtained in step (1) into 2 / 3 of the water, stir and heat to 80°C to dissolve Uniform;

[0024] (3) Add the remaining 1 / 3 of water and mix well;

[0025] (4) The mixture obtained in step (3) was sterilized at 121° C. for 30 minutes at high temperature, and then cooled to room temperature to obtain the culture medium.

Embodiment 2

[0027] Raw materials are weighed according to the following percentages by weight: Jerusalem artichoke stem and leaf 20%, potato 3%, beef extract 3%, fish meal 4%, vitamin B1 0.05%, riboflavin 0.04%, folic acid 0.03%, magnesium sulfate 0.08%, sodium chloride 0.08%, the balance water;

[0028] (1) Pulverizing the stem and leaf of Jerusalem artichoke and crossing a 100-mesh sieve to obtain Jerusalem artichoke powder;

[0029] (2) Add Jerusalem artichoke powder, potato, beef extract, fish meal, vitamin B1, riboflavin, folic acid, magnesium sulfate and sodium chloride obtained in step (1) into 2 / 3 of the water, stir and heat to 90°C to dissolve Uniform;

[0030] (3) Add the remaining 1 / 3 of water and mix well;

[0031] (4) The mixture obtained in step (3) was sterilized at 121° C. for 30 minutes at high temperature, and then cooled to room temperature to obtain the culture medium.

Embodiment 3

[0033] Raw materials are weighed according to the following percentages by weight: 30% of Jerusalem artichoke tubers, 2% of potatoes, 2% of beef extract, 3% of fish meal, 0.03% of vitamin B1, 0.02% of riboflavin, 0.02% of folic acid, 0.05% of magnesium sulfate, and 0.06% of sodium chloride %, the balance of water;

[0034] (1) the Jerusalem artichoke tuber is pulverized and crossed a 100-mesh sieve to obtain the Jerusalem artichoke powder;

[0035] (2) Add Jerusalem artichoke powder, potato, beef extract, fish meal, vitamin B1, riboflavin, folic acid, magnesium sulfate and sodium chloride obtained in step (1) into 2 / 3 of the water, stir and heat to 100°C to dissolve Uniform;

[0036] (3) Add the remaining 1 / 3 of water and mix well;

[0037] (4) The mixture obtained in step (3) was sterilized at 121° C. for 30 minutes at high temperature, and then cooled to room temperature to obtain the culture medium.

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PUM

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Abstract

The invention relates to a cordyceps sinensis fluid medium and a preparation method thereof. The cordyceps sinensis fluid medium comprises the following materials by weight percent: 10-30% of jerusalem artichoke, 1-5% of potato, 1-5% of beef extract, 1-6% of fish meal, 0.03-0.08% of vitamin B1, 0.02-0.06% of riboflavin, 0.01-0.05% of folic acid, 0.05-0.1% of magnesium sulfate, 0.06-0.1% of sodium chloride, and the balance of water. The jerusalem artichoke powder is firstly used for the cordyceps sinensis fluid fermentation medium, an excellent technological effect is acquired, the quality of the obtained cordyceps sinensis is high, and active ingredients of the obtained cordyceps sinensis are rich.

Description

technical field [0001] The invention relates to the technical field of fermentation production of edible and medicinal fungi, in particular to a liquid culture medium of Cordyceps sinensis and a preparation method thereof. Background technique [0002] Cordyceps sinensis is formed by connecting the insect body and the sub-seat, the total length is 9-12 cm, the color is dark brown, and there are many horizontal wrinkles on the back, the insect body is light and brittle, easy to break, the section is white, slightly elastic; The head of the body protrudes and is 4-7cm long. The base covers the head of the body and is in the shape of a long rod, thick at the top and thin at the bottom, taupe or dark brown, flexible and flexible, and the cross-section is mostly hollow. Smell with straw mushroom aroma, sweet. Semen solitary, slender cylindrical, 4 to 7 cm long, about 3 mm in diameter; surface dark brown to tan, with fine longitudinal wrinkles, upper part slightly enlarged, soft ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 王玉花王辉杨成香谢建军王晓燕王茜
Owner 青海珠峰冬虫夏草工程技术研究有限公司
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