Cordyceps sinensis fluid medium and preparation method thereof
A liquid culture medium, the technology of Cordyceps sinensis, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor quality of Cordyceps sinensis, differences in mycelium properties, low nutrient content, etc., and achieve excellent technical results Effect
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Embodiment 1
[0021] Raw materials are weighed according to the following percentages by weight: 10% of Jerusalem artichoke tubers, 5% of potatoes, 5% of beef extract, 6% of fish meal, 0.08% of vitamin B1, 0.06% of riboflavin, 0.05% of folic acid, 0.1% of magnesium sulfate, and 0.1% of sodium chloride %, the balance of water;
[0022] (1) the Jerusalem artichoke tuber is pulverized and crossed a 100-mesh sieve to obtain the Jerusalem artichoke powder;
[0023] (2) Add Jerusalem artichoke powder, potato, beef extract, fish meal, vitamin B1, riboflavin, folic acid, magnesium sulfate and sodium chloride obtained in step (1) into 2 / 3 of the water, stir and heat to 80°C to dissolve Uniform;
[0024] (3) Add the remaining 1 / 3 of water and mix well;
[0025] (4) The mixture obtained in step (3) was sterilized at 121° C. for 30 minutes at high temperature, and then cooled to room temperature to obtain the culture medium.
Embodiment 2
[0027] Raw materials are weighed according to the following percentages by weight: Jerusalem artichoke stem and leaf 20%, potato 3%, beef extract 3%, fish meal 4%, vitamin B1 0.05%, riboflavin 0.04%, folic acid 0.03%, magnesium sulfate 0.08%, sodium chloride 0.08%, the balance water;
[0028] (1) Pulverizing the stem and leaf of Jerusalem artichoke and crossing a 100-mesh sieve to obtain Jerusalem artichoke powder;
[0029] (2) Add Jerusalem artichoke powder, potato, beef extract, fish meal, vitamin B1, riboflavin, folic acid, magnesium sulfate and sodium chloride obtained in step (1) into 2 / 3 of the water, stir and heat to 90°C to dissolve Uniform;
[0030] (3) Add the remaining 1 / 3 of water and mix well;
[0031] (4) The mixture obtained in step (3) was sterilized at 121° C. for 30 minutes at high temperature, and then cooled to room temperature to obtain the culture medium.
Embodiment 3
[0033] Raw materials are weighed according to the following percentages by weight: 30% of Jerusalem artichoke tubers, 2% of potatoes, 2% of beef extract, 3% of fish meal, 0.03% of vitamin B1, 0.02% of riboflavin, 0.02% of folic acid, 0.05% of magnesium sulfate, and 0.06% of sodium chloride %, the balance of water;
[0034] (1) the Jerusalem artichoke tuber is pulverized and crossed a 100-mesh sieve to obtain the Jerusalem artichoke powder;
[0035] (2) Add Jerusalem artichoke powder, potato, beef extract, fish meal, vitamin B1, riboflavin, folic acid, magnesium sulfate and sodium chloride obtained in step (1) into 2 / 3 of the water, stir and heat to 100°C to dissolve Uniform;
[0036] (3) Add the remaining 1 / 3 of water and mix well;
[0037] (4) The mixture obtained in step (3) was sterilized at 121° C. for 30 minutes at high temperature, and then cooled to room temperature to obtain the culture medium.
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