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Fermentation method for producing gamatine

A technology of agmatine and fermentation liquid, applied in the field of bioengineering, can solve the problems of low yield, pollution, and many steps in the chemical method route, and achieve the effect of ensuring food safety and broad industrial application prospects

Active Publication Date: 2016-11-23
洛阳华荣生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently used in the industry are all produced by chemical methods, which have many routes, low yields, and serious pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 : Preculture and fermentation

[0042] The bacterial strain CGMCC No.12601 preservation solution packed in the glycerol tube was thawed, and inoculated in the seed medium (glucose 75g / L, corn steep liquor 25-30g / L, (NH4) 2 SO 4 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5g / L, urea 1.0g / L, histidine 30mg / L, molasses 25g / L, biotin 100μg / L, pH 7.0), 5000mL Erlenmeyer bottle liquid volume 500mL, 30 ℃ for 18 hours, absorbance value When ΔOD=0.4-0.5, shake the bed to obtain seed culture solution. After testing, no bacteria.

[0043] Inoculate 500 mL of seed culture solution into a 7-liter fermenter with a liquid volume of 5 liters. The composition of the medium is the same as that of the seed medium, pH=6.4-6.7 (after sterilization). Feed medium: ammonium sulfate 500g / L, glucose 650g / L. The culture temperature is 30°C, the tank pressure is 0.05Mpa, and the initial aeration ratio is 1vvm. Stirring 600rpm. During the fermentation, the pH is controlled at...

Embodiment 2

[0044] Example 2 : Separation of fermentation broth

[0045] 5.2 liters of fermented broth obtained in Example 1 was centrifuged at 4500 rpm, and the thallus was discarded. Add 1% activated carbon to the supernatant, stir and decolorize for 30 minutes, then add 1% diatomaceous earth and filter to obtain a clear filtrate.

Embodiment 3

[0046] Example 3 : Extraction of Agmatine

[0047] The filtrate obtained in Example 2 was passed through strongly acidic cation exchange resin 732 at a rate of 1 column volume per hour, and agmatine was exchanged and adsorbed on the resin column. After washing with pure water for one column volume, 5% sulfuric acid was used for elution, and the eluate was collected.

[0048] The eluate was adjusted to pH=6.0 with ammonia water, 1% activated carbon was added, stirred for 30 minutes to decolorize, and suction filtered. The filtrate was collected and concentrated under reduced pressure. When concentrated to about 800g / L, it was cooled to room temperature to crystallize for more than 4 hours, and the crystals were collected by suction filtration to obtain 347 grams of wet crystals of agmatine sulfate.

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PUM

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Abstract

The invention discloses a one-step fermentation method for producing gamatine through microorganism. A non-pathogenic microorganism is adopted to directly convert a carbon source and a nitrogen source to the gamatine through fermentation, and the produced gamatine can be further prepared into salt, such as sulfate, hydrochloride, phosphate, nitrate and orotic acid salt which are used as nutritious supplements, so that the method has a wide industrial application prospect. The microorganism used in the method comprises corynebacterium glutamicum constructed through gene engineering and preserved with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 12601 in General Microbiology Center of China Committee for Culture Collection of Microorganisms.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for producing agmatine through microbial fermentation. Background technique [0002] Agmatine, also known as herring spermine, agmatine, 1-(4-aminobutyl)guanidine, is a biologically active substance naturally occurring in organisms, widely present in all tissues and organs of mammals , Exercising a variety of physiological functions in the cardiovascular system, gastrointestinal system, central nervous system, etc. As a supplement for maintaining sports nutrition, agmatine is superior to arginine in many aspects in function, and can help users improve body endurance during exercise, accelerate body recovery after exercise, and improve body composition. Including increasing muscle mass and reducing fat fat, so it is widely used in medicine and health care products market. Agmatine also has a variety of pharmacological activities such as lowering blood sugar, lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12N1/20C12R1/15
CPCC12P13/001C12N1/205C12R2001/15
Inventor 范文超
Owner 洛阳华荣生物技术有限公司
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