Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing biodiesel by taking dunaliella salina as raw material

A Dunaliella salina and biodiesel technology, applied in the field of biodiesel preparation, can solve the problems of low biodiesel yield, low catalytic efficiency, and long reaction time, and achieve the effects of strong environmental adaptability, improved catalytic efficiency, and large reaction interface

Inactive Publication Date: 2016-12-07
南宁华侨投资区政孙贸易有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing biodiesel production process generally has the following problems: low catalytic efficiency, low biodiesel yield, long reaction time, corrosion equipment, complex process, high energy consumption, high cost, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of Dunaliella salina medium: glucose 20mg / L, NaNO 3 2.4mg / L, KH 2 PO 4 1.0mg / L, Na 2 SiO 3 1.0mg / L, chitosan 0.6mg / L, biogas slurry 10mg / L, f2 vitamin 1mL and seawater 1L;

[0027] A method for preparing biodiesel with Dunaliella salina as raw material, comprising the following steps:

[0028] S1. Dunaliella salina culture: use Dunaliella salina as raw material, inoculate it into the culture medium at a seeding density of 4 million cells / mL, and irradiate it with red and blue dual-color LED supplementary light for 16h / day, in which the ratio of red light to blue light is Control the light intensity at 1:1 at 3500LX, control the culture temperature at 25°C, cultivate for 12 days, the density of Dunaliella salina reaches 90 million cells / mL, and collect Dunaliella salina cells;

[0029] S2. Oil extraction: Concentrate and dry the Dunaliella salina cells collected in step S1, add a high-speed ball mill and crush at a speed of 500r / min for 25 minutes to ob...

Embodiment 2

[0034] Preparation of Dunaliella salina medium: glucose 25mg / L, NaNO 3 2.0mg / L, KH 2 PO 4 1.1mg / L, Na 2 SiO 3 1.2mg / L, chitosan 0.7mg / L, biogas slurry 8mg / L, f2 vitamin 3mL and seawater 1L;

[0035] A method for preparing biodiesel with Dunaliella salina as raw material, comprising the following steps:

[0036] S1. Dunaliella salina culture: use Dunaliella salina as raw material, inoculate it into the culture medium at an inoculation density of 5 million cells / mL, and irradiate it with red and blue dual-color LED supplementary light for 18h / day, in which the ratio of red light to blue light is Control the light intensity at 3700LX for 2:1, control the culture temperature at 26°C, cultivate for 10 days, the density of Dunaliella salina reaches 90 million cells / mL, and collect Dunaliella salina cells;

[0037] S2. Grease extraction: After concentrating and drying the Dunaliella salina cells collected in step S1, put them into a high-speed ball mill and crush them at a speed ...

Embodiment 3

[0042] Preparation of Dunaliella salina culture medium: glucose 30mg / L, NaNO 3 1.8mg / L, KH 2 PO 4 1.2mg / L, Na 2 SiO 3 1.5mg / L, chitosan 0.8mg / L, biogas slurry 6mg / L, f2 vitamin 2mL and seawater 1L;

[0043] A method for preparing biodiesel with Dunaliella salina as raw material, comprising the following steps:

[0044] S1. Dunaliella salina culture: use Dunaliella salina as raw material, inoculate it into the culture medium at an inoculation density of 6 million cells / mL, and irradiate it with red and blue dual-color LED supplementary light for 20h / day, in which the ratio of red light to blue light is Control the light intensity at 4000LX for 3:1, control the culture temperature at 27°C, cultivate for 12 days, the density of Dunaliella salina reaches 90 million cells / mL, and collect Dunaliella salina cells;

[0045] S2. Oil extraction: After concentrating and drying the Dunaliella salina cells collected in step S1, add a high-speed ball mill to crush at a speed of 800r / m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing biodiesel by taking dunaliella salina as the raw material. The method comprises the following steps that the dunaliella salina is inoculated onto a culture medium, a red and blue double color LED light is used for irradiation for 16 h / d to 20 h / d, culturing is conducted for 10 d to 12 d at 25 DEG C to 27 DEG C, and dunaliella salina cells can be collected; the collected dunaliella salina cells are added into a high speed ball mill for crushing, extraction is conducted through an organic solvent, and microalgal oil is obtained; the microalgal oil, methyl alcohol and a zeolite molecular sieve catalyst are added into a reactor for an esterification reaction according to the mass ratio of 1:(3-5):(0.03-0.05), and an esterification product is obtained; the esterification product is subjected to separation and purification, and the biodiesel is obtained. According to the method for preparing the biodiesel by taking the dunaliella salina as the raw material, the dunaliella salina which is short in production cycle, wide in distribution, high in photosynthesis and high in oil content serves as the raw material, and the problem that the plant oil raw material in development of the biodiesel exists is solved. Meanwhile, the advantages of being simple in preparation technology, high in catalytic efficiency, high in oil extraction oil, high in production yield and the like are achieved, and a good foundation is laid for industrialization and large scale production of the biodiesel.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and specifically relates to a method for preparing biodiesel from Dunaliella salina as a raw material. Background technique [0002] With the increasing depletion of fossil fuels and the sharp rise in oil prices, the search for renewable energy has become a solution to human survival and to maintain the sustainable development of the world economy. As a new alternative energy, biodiesel is developing rapidly in countries all over the world. Biodiesel refers to oil crops such as soybeans, rapeseed, cotton, palm, etc., wild oil plants and engineering microalgae and other aquatic vegetable oils, animal fats, and catering waste oils, etc. Renewable diesel fuel to replace petrodiesel. The advantages of biodiesel are not limited to renewability. Compared with existing diesel, it also has the following advantages: high cetane number, which makes the combustion performance better; better wetting ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/649Y02E50/10
Inventor 黄健峻其他发明人请求不公开姓名
Owner 南宁华侨投资区政孙贸易有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com