Full-oxygen bacteria package hybridization tissue culture active liquid inoculation technology

Abstract

The invention provides a full-oxygen bacteria package hybridization tissue culture active liquid inoculation technology and belongs to the technical field of edible fungi production. The technology is as below: subjecting edible and medicinal fungi to liquid fermentation; liquid fermentation refers to: in a biochemical reactor, dissolving the sugars, organics and inorganic nitrogen-containing compounds, some trace elements of inorganic salts and other nutrients necessary in the growth process of the edible and medicinal fungi in the nature in water as a medium; sterilizing, inoculating strains, introducing aseptic air, stirring to provide the oxygen for edible fungi respiration, controlling the suitable external conditions, and conducting a mass culture reproduction process of mycelia. The technology does not need artificial acupuncture for ventilation or artificial prick for discharge of yellow water, and has good thermal insulation performance, multiple mushroom batches; one charge and inoculation can maintain continuous of fresh mushroom harvest for 3-6 months according to the different types of mushroom; twice inoculation in one year and full-oxygen ventilation bacteria culture are carried out, the production of the mycelia is rapid, so as to improve the speed by more than 50%.

Description

technical field [0001] The invention belongs to the technical field of edible fungus production, and in particular relates to an active liquid inoculation technology for the hybrid tissue culture of an aerobic bacteria package. Background technique [0002] Liquid strains have the advantages of convenience, short bacterial age, high purity, and strong vitality, and are more and more valued by scientific researchers and the majority of edible fungi producers. There are several inoculation methods: 1. Material surface inoculation method: This is currently the most widely used method. When using this inoculation method, it is mostly bottled or bagged compost, and there is a certain space at the top, such as the production of Flammulina velutipes, Agaricus, black fungus, Pleurotus eryngii, and Lentinus edodes, Coprinus comatus, and Pleurotus eryngii. When inoculating various strains, try to spray the surface of the material with the strains. The liquid seeds germinate and grow q...

AI Technical Summary

Problems solved by technology

3. The inoculation method of pricking holes on the surface of the bag: Inoculation of long bacteria sticks from both ends can not shorten the time of bacteria cultivation. From one or both sides of the surface of the bag, use the gun tip to pierce 3-5cm deep and inoculate at the same time. The interval between pricking holes is determined according to needs. For example, 3-5 holes are pricked in each stick of shiitake mushrooms. This method can shorten the cultivation time and reduce the contamination of bacteria by adjusting the amount of inoculum and the distance between the holes. In 10ml, if not sealed after inoculation, it should reach more than 20ml per well

[0003] Although the above-mentioned inoculation methods have prevailed and can meet the needs of general production, some of the above-mentioned methods require manual acupuncture for ventilation and oxygen supply, some require manual piercing of holes to drain yellow water, some have poor thermal insulation and moisture retention performance, and some have Mushroom batches are small, which cannot meet the needs of mass production