Method for preparing resveratrol from danfeng paeonia suffruticosa andr seed shell
A technology for resveratrol and peony seed shells, which is applied in chemical instruments and methods, preparation of organic compounds, anti-toxic agents, etc., can solve problems such as low utilization rate and discarding, and achieves prevention of decomposition, easy implementation, and simple process operation. Effect
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Embodiment 1
[0032] Dry the peony seed husks until the water content is less than 8%, crush them, and sieve them with a 10-60 mesh sieve to obtain fine powder of peony peony seed husks; add 50%-95% of the volume fraction of Ethanol aqueous solution, the mass volume ratio of the two is 1:8~1:30 (g:mL), then use ultrasonic extraction for 60~120min, the extraction temperature is 40℃~90℃, extract 1~3 times, filter, combine the filtrate, The filtrate was evaporated under reduced pressure at 40-60°C, the solvent was recovered, and the concentrate was vacuum-dried or freeze-dried to obtain the crude extract of resveratrol; 10 (g:mL) was added to distilled water, stirred and dissolved at 60°C, filtered, and the filtrate was extracted with petroleum ether at a volume ratio of 1:1 for 3 to 5 times, combined with petroleum ether extracts, concentrated under reduced pressure at 40 to 60°C to obtain petroleum ether extract; the water layer was extracted 3 to 5 times with equal volumes of ethyl acetate,...
Embodiment 2
[0037] Dissolve the ethyl acetate extract in distilled water, the volume ratio of the ethyl acetate extract to water is 0.8-3.0 mg / mL, adjust the pH of the solution to 1.0-4.0, the quality of macroporous resin X-5 and ethyl acetate extract aqueous solution The volume ratio is 1:0.5~10 (mg:mL), the adsorption rate of the ethyl acetate extract aqueous solution on the column is 1~6 mL / min, after the sample is adsorbed, the macroporous resin column is eluted with 50~90% ethanol, and the The de-agent flow rate is 1-3mL / min, the amount of eluent is 1-4 times the volume of the macroporous resin, and the resveratrol polyphenol fraction is collected and analyzed by HPLC to obtain the Resveratrol polyphenol extract composed of paeonol, rutin and quercetin.
[0038] HPLC analysis conditions: HPLC column is Hypersil GOLD aQ C 18Φ250×4.6mm, 5μm, mobile phase: volume ratio of methanol to 0.5% glacial acetic acid aqueous solution is 4:6; UV wavelength is 306nm; column temperature: 30°C; flo...
Embodiment 3
[0042] Dry the peony seed husks until the water content is less than 8%, crush them, and sieve them with a 10-60 mesh sieve to obtain fine powder of peony peony seed husks; add 50%-95% of the volume fraction of Ethanol aqueous solution, the mass volume ratio of the two is 1:8~1:30 (g:mL), then use microwave extraction for 60~120min, the extraction temperature is 40℃~90℃, extract 1~3 times, filter, combine the filtrate, The filtrate was evaporated under reduced pressure at 40-60°C, the solvent was recovered, and the concentrate was vacuum-dried or freeze-dried to obtain the crude extract of resveratrol; 10 (g:mL) was added to distilled water, stirred and dissolved at 60°C, filtered, and the filtrate was extracted with petroleum ether at a volume ratio of 1:1 for 3 to 5 times, combined with petroleum ether extracts, concentrated under reduced pressure at 40 to 60°C to obtain petroleum ether extract; the water layer was extracted with equal volume of ethyl acetate for 3 to 5 time...
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