Method for preparing resveratrol from danfeng paeonia suffruticosa andr seed shell

A technology for resveratrol and peony seed shells, which is applied in chemical instruments and methods, preparation of organic compounds, anti-toxic agents, etc., can solve problems such as low utilization rate and discarding, and achieves prevention of decomposition, easy implementation, and simple process operation. Effect

Inactive Publication Date: 2016-12-21
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Oil peony seed shells are partially used as fertilizers, but more are discarded, and the utilization rate is extremely low
At present, there are very few academic reports on

Method used

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  • Method for preparing resveratrol from danfeng paeonia suffruticosa andr seed shell
  • Method for preparing resveratrol from danfeng paeonia suffruticosa andr seed shell
  • Method for preparing resveratrol from danfeng paeonia suffruticosa andr seed shell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Dry the peony seed husks until the water content is less than 8%, crush them, and sieve them with a 10-60 mesh sieve to obtain fine powder of peony peony seed husks; add 50%-95% of the volume fraction of Ethanol aqueous solution, the mass volume ratio of the two is 1:8~1:30 (g:mL), then use ultrasonic extraction for 60~120min, the extraction temperature is 40℃~90℃, extract 1~3 times, filter, combine the filtrate, The filtrate was evaporated under reduced pressure at 40-60°C, the solvent was recovered, and the concentrate was vacuum-dried or freeze-dried to obtain the crude extract of resveratrol; 10 (g:mL) was added to distilled water, stirred and dissolved at 60°C, filtered, and the filtrate was extracted with petroleum ether at a volume ratio of 1:1 for 3 to 5 times, combined with petroleum ether extracts, concentrated under reduced pressure at 40 to 60°C to obtain petroleum ether extract; the water layer was extracted 3 to 5 times with equal volumes of ethyl acetate,...

Embodiment 2

[0037] Dissolve the ethyl acetate extract in distilled water, the volume ratio of the ethyl acetate extract to water is 0.8-3.0 mg / mL, adjust the pH of the solution to 1.0-4.0, the quality of macroporous resin X-5 and ethyl acetate extract aqueous solution The volume ratio is 1:0.5~10 (mg:mL), the adsorption rate of the ethyl acetate extract aqueous solution on the column is 1~6 mL / min, after the sample is adsorbed, the macroporous resin column is eluted with 50~90% ethanol, and the The de-agent flow rate is 1-3mL / min, the amount of eluent is 1-4 times the volume of the macroporous resin, and the resveratrol polyphenol fraction is collected and analyzed by HPLC to obtain the Resveratrol polyphenol extract composed of paeonol, rutin and quercetin.

[0038] HPLC analysis conditions: HPLC column is Hypersil GOLD aQ C 18Φ250×4.6mm, 5μm, mobile phase: volume ratio of methanol to 0.5% glacial acetic acid aqueous solution is 4:6; UV wavelength is 306nm; column temperature: 30°C; flo...

Embodiment 3

[0042] Dry the peony seed husks until the water content is less than 8%, crush them, and sieve them with a 10-60 mesh sieve to obtain fine powder of peony peony seed husks; add 50%-95% of the volume fraction of Ethanol aqueous solution, the mass volume ratio of the two is 1:8~1:30 (g:mL), then use microwave extraction for 60~120min, the extraction temperature is 40℃~90℃, extract 1~3 times, filter, combine the filtrate, The filtrate was evaporated under reduced pressure at 40-60°C, the solvent was recovered, and the concentrate was vacuum-dried or freeze-dried to obtain the crude extract of resveratrol; 10 (g:mL) was added to distilled water, stirred and dissolved at 60°C, filtered, and the filtrate was extracted with petroleum ether at a volume ratio of 1:1 for 3 to 5 times, combined with petroleum ether extracts, concentrated under reduced pressure at 40 to 60°C to obtain petroleum ether extract; the water layer was extracted with equal volume of ethyl acetate for 3 to 5 time...

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Abstract

The invention relates to a method for preparing resveratrol from danfeng paeonia suffruticosa andr seed shell, and belongs to the technical field of natural products, wherein danfeng paeonia suffruticosa andr seed shell is adopted as a raw material, and extracting, extraction, macroporous resin purification and silica gel column chromatography separation are performed to obtain the danfeng paeonia suffruticosa andr seed shell resveratrol. According to the present invention, the danfeng paeonia suffruticosa andr seed shell resveratrol prepared through the technical conditions of the present invention has characteristics of simple preparation process, high purity, cheap raw material, and the like; and the prepared danfeng paeonia suffruticosa andr seed shell polyphenol extract has characteristics of good anti-oxidation activity, good bacterial inhibition activity and good commercial prospect, and can be widely used in the field of food and skin care products.

Description

technical field [0001] The invention relates to a method for preparing resveratrol from danfeng peony seed shells, belonging to the technical field of natural products. Background technique [0002] peony( Paeonia suffruticosa Andr.) belongs to Paeoniaceae (Paeoniaeeae), Paeoniaceae ( Paeonia ) Perennial woody plants. Peony root bark has very good medicinal value, which was recorded in "Materia Medica" two thousand years ago. Until the Sui and Tang Dynasties, peony became a rare ornamental plant. Peony seeds are the seeds of peony flower knots. They are black in color, hard in skin, bitter in taste, irregularly round, and slightly larger than soybeans. The peony seed shell is the black hard shell on the surface of peony seeds. Peony seeds can treat waist and leg pain in the folk, and have anti-inflammatory and anti-oxidative effects. The chemical and pharmacological studies of traditional Chinese medicine have found that peony seeds have antibacterial, anti-inflammat...

Claims

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Application Information

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IPC IPC(8): C07C37/68C07C37/82C07C37/72C07C39/21A61P39/06A61P31/04
CPCC07C37/685C07C37/72C07C37/82C07C39/21
Inventor 王成章张红玉陈虹霞
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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