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Application of alkaloidal compound

A technology of alkaloids and compounds, applied in the field of medicine, can solve the problems of lag in the development and use of influenza vaccines, increase the risk of bronchospasm, and have adverse reactions, so as to prolong the average survival time, relieve the symptoms of lung infection, and prolong the survival time. Effect

Active Publication Date: 2016-12-21
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Amantadine and rimantadine only have inhibitory effects on influenza A virus, and there are adverse reactions
Oseltamivir is used as a first-line anti-influenza virus drug, but it has been found that oseltamivir-resistant strains have emerged and spread, and the price is expensive
Zanamivir is mainly used in the early treatment of influenza, but is not suitable for children, and may increase the risk of bronchospasm in patients with asthma or chronic obstructive pulmonary disease
The vaccine only has a preventive effect on known influenza virus subtypes, and is ineffective against new influenza viruses produced by antigenic drift or antigenic conversion. The development and use of influenza vaccines are relatively lagging behind

Method used

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  • Application of alkaloidal compound
  • Application of alkaloidal compound
  • Application of alkaloidal compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Cytotoxicity of Peiminin

[0028] 1. Experimental materials

[0029] Cell and culture method: African green monkey kidney cells (Vero) were used as the cell model, and DMEM medium containing 10% fetal bovine serum (Jiangsu Kaiji Biotechnology Co., Ltd., batch number: 150713) was used, placed at 37°C, 5 %CO 2 Cultured in an incubator, when the cells grow to 90% density, trypsinize (Gibco company, lot number: 1535318) and subculture, the subculture ratio is 1 / 3-1 / 4.

[0030] Reagents: MTS Cell Proliferation Quantitative Detection Kit, purchased from Promega Company (batch number: 0000158071); Fritillarin, purchased from Shanghai Eternal Biotechnology Co., Ltd., batch number YH20150425.

[0031] Instruments: microplate reader, purchased from Molecular Devices, model: Spectra Max M2e; inverted microscope, purchased from Olympus, model: CKX41; carbon dioxide incubator, purchased from Thermo Scientific, model: Forma Steri-Cycle 371; Biological safety cabinets we...

Embodiment 2

[0041] Example 2: Protection and inhibition of virus replication of EV71 and CoxA16 virus-infected Vero cells by peiminin

[0042] 1. Experimental materials

[0043] African green monkey kidney cells (Vero) were used as the cell model, and DMEM medium containing 10% fetal bovine serum (Jiangsu Kaiji Biotechnology Co., Ltd., batch number: 150713) was used, and placed in a 37°C, 5% CO2 incubator After culturing, when the cells grow to 90% density, trypsinize (Gibco company, batch number: 1535318) and subculture, the subculture ratio is 1 / 3-1 / 4.

[0044] EV71 virus BJ09 / 07 strain, GenBank accession number JQ319054.1, CoxA16 virus TS10 / 08 strain, GenBank accession number JX068829. The median cytopathic dose (TCID50) of EV71 virus was determined to be 108 / mL before use, and the CoxA16 TCID50 was 108.5 / mL.

[0045] MTS Cell Proliferation Quantitative Detection Kit was purchased from Promega Company (Lot No.: 0000158071); Peiminin was purchased from Shanghai Eternal Biotechnology C...

Embodiment 3

[0059] Embodiment 3: The therapeutic effect of peiminin on EV71 virus infection

[0060] 1. Experimental materials

[0061] 5-day-old ICR mice (Mus musculus) were purchased from Qinglongshan Animal Breeding Farm, Jiangning District, Nanjing. EV71 virus BJ09 / 07 strain, GenBank accession number JQ319054.1. Determine the half cytopathic dose (TCID) of EV71 virus before use 50 ) is 10 8 / mL.

[0062] Ribavirin injection (Chenxin Pharmaceutical Co., Ltd., batch number: 140903642, RBV), physiological saline for injection, and disposable sterile syringes. Cytokines IL-6 (lot number: 117467012), TNF-α (lot number: 116660009) and MCP-1 (lot number: 105130013) ELISA assay kits were purchased from eBioscience.

[0063] 2. Experimental method

[0064] (1) Virus liquid preparation: according to virus TCID 50 value, the virus was diluted to 10 before use 7 TCID 50 / mL.

[0065] (2) Preparation of medicinal solution: fritillaria (purchased from Chengdu Pufeide Biotechnology Co., Ltd....

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Abstract

The invention relates to the field of medical technology, and particularly discloses application of an alkaloidal compound with a structure as shown in a formula I to the preparation of a medicine for curing a virus disease or to the preparation of a virus inhibitor. The alkaloidal compound with the structure as shown in the formula I is prepared from fritillary bulb, namely a natural Chinese medicinal herb, the side effect is small, the alkaloidal compound has an effect of inhibiting an enterovirus and an influenza virus and has a better effect of curing the virus diseases caused by the enterovirus and the influenza virus, and a new way for curing a hand foot and mouth disease and the influenza through the Chinese herbal medicinal ingredient is provided.

Description

technical field [0001] The invention relates to the technical field of medicine, and more specifically relates to the application of alkaloid compounds. Background technique [0002] Hand, foot and mouth disease (HFMD) is a common infectious disease caused by a variety of enteroviruses, mainly through the digestive tract, respiratory tract and close contact, and mainly affects infants and young children. HFMD is mainly caused by the infection of Coxsackievirus A16 (CoxA 16) and Enterovirus 71 (EV 71), which belong to the genus Enterovirus of the RNA virus family, and EV 71 is the most serious. HFMD mainly manifests as fever with myoclonus, oral mucosal ulcerative herpes, and vesicular rash at the extremities. In severe cases, it can cause encephalitis, brainstem encephalitis, polio-like paralysis, acute flaccid paralysis, etc. The disease is seriously harmful. [0003] At present, although the vaccine against EV71 virus has been approved for marketing, there is still no dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D455/03A61P1/00A61P31/12A61P31/16
CPCC07D455/03
Inventor 萧伟曹泽彧丁玥孙兰曹亮王振中丁岗胡晗绯
Owner JIANGSU KANION PHARMA CO LTD
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