Method for extracting probiotic cell wall skeleton polysaccharide
A probiotics, cell wall technology, applied in the field of biological extraction, can solve the problems of long time, complicated operation and high cost
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Embodiment 1
[0023] Embodiment 1 A kind of method for extracting probiotic cell wall skeleton polysaccharide
[0024] (1) Preparation of bacteria:
[0025] MRS medium formula: peptone 10g, beef powder 10g, yeast extract powder 5g, K 2 HPO 4 2g, anhydrous sodium acetate 5g, glucose 20g, MnSO 4 ·7H 2 O 0.58g, MnSO 4 0.25g, diamine limonene 2g, distilled water 1000ml.
[0026] Preparation method: 1) Prepare 2800ml of MRS medium according to the proportion of the above formula, put it in an autoclave, sterilize at 115°C for 20 minutes, and set aside;
[0027] 2) Pick the Lactobacillus rhamnosus preserved in the laboratory in 50ml of MRS medium, then place it in a constant temperature shaking incubator, and shake it at 37°C for 12 hours;
[0028] 3) Then inoculate the bacterial solution in 2750ml MRS medium according to the inoculum amount of 2%, then place it in a constant temperature shaking incubator, and shake it at 37°C for 12 hours;
[0029] 4) Centrifuge the cultured Lactobacill...
Embodiment 2
[0069] Embodiment 2 A kind of method for extracting probiotic cell wall skeleton polysaccharide
[0070] (1) Preparation of bacteria:
[0071] MRS medium formula: peptone 10g, beef powder 10g, yeast extract powder 5g, K 2 HPO 4 2g, anhydrous sodium acetate 5g, glucose 20g, MnSO 4 ·7H 2 O 0.58g, MnSO 4 0.25g, diamine limonene 2g, distilled water 1L.
[0072] Preparation method: 1) Prepare MRS medium according to the above formula ratio, place it in an autoclave, sterilize at 115°C for 20 minutes, and set aside;
[0073] 2) Pick the Lactobacillus rhamnosus preserved in the laboratory in 10ml of MRS medium, then place it in a constant temperature shaking incubator, and shake it at 37°C for 12 hours;
[0074] 3) Inoculate the bacterial solution in 100ml MRS medium according to the inoculum amount of 2%, then place it in a constant temperature shaking incubator, and shake it at 37°C for 12 hours;
[0075] 4) Inoculate the bacterial solution in 3L MRS medium according to th...
Embodiment 3
[0149] Embodiment 3 A kind of method for extracting probiotic cell wall skeleton polysaccharide
[0150] The method is the same as in Example 1, except that Lactobacillus rhamnosus is replaced by Lactobacillus reuteri, Lactobacillus acidophilus, Bifidobacterium animalis and Bifidobacterium lactis, respectively, to obtain four kinds of dry probiotic cell wall skeleton polysaccharide powders. After weighing and freeze-drying, the purity and yield of each bacterial cell obtained are shown in Table 4.
[0151] Table 4
[0152]
PUM
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