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Reversible regulation method for yeast flocculation and applications of reversible regulation method

A yeast and flocculation technology, applied in the field of reversible regulation of yeast flocculation, can solve the problems of low fermentation rate, low recovery activity, genetic instability, etc., and achieve the effects of easy aging, increase the number of recycling and use, and prepare raw materials economically.

Active Publication Date: 2016-12-21
广东省科学院生物与医学工程研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The primary purpose of the present invention is to overcome the shortcomings and deficiencies of the prior art, provide a reversible control method for yeast flocculation, realize energy-saving separation of non-self-flocculating S. The re-fermentation of cells solves the problems of low fermentation rate, genetic instability, and low recovery activity when flocculated Saccharomyces cerevisiae is used in the alcoholic fermentation process. avoid the disadvantages of both

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  • Reversible regulation method for yeast flocculation and applications of reversible regulation method
  • Reversible regulation method for yeast flocculation and applications of reversible regulation method
  • Reversible regulation method for yeast flocculation and applications of reversible regulation method

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Embodiment 1

[0078] 1. The reversible control method of yeast flocculation provided by the present invention is as follows: figure 1 As shown, the specific steps are as follows:

[0079] (1) Seed yeast culture collection stage: the sugarcane molasses (Guangdong Xuwen Sanhe Development Co., Ltd.) with a solid content of 85 ° of Brix is ​​diluted to 10 ° of Brix with distilled water, and urea is added by mass volume ratio 0.2% (Guangzhou Chemical Reagent Factory ), the pH value was adjusted to 5.0 with concentrated sulfuric acid (concentration: 98% by mass, Guangzhou Chemical Reagent Factory), and 50 mL was sterilized at 115° C. for 20 min as a seed medium. Under sterile conditions, connect a ring of fresh Saccharomyces cerevisiae lawn (AS2.1190) to the seed medium, culture at 30°C and 100 rpm for 16 hours, and the measured number of yeasts is 85 million / mL to obtain yeast seed liquid.

[0080] (2) Preparation of flocculant and deflocculant: Heat 85°Brix sugarcane molasses in a water bath a...

Embodiment 2

[0091] 1. The reversible control method of yeast flocculation provided by the present invention is as follows: figure 1 As shown, the specific steps are as follows:

[0092] (1) Seed yeast culture and collection stage: Weigh 1.5g of cassava flour (Zhongshan Hongxin Biotechnology Co., Ltd.), add 50mL of distilled water, gelatinize in a water bath at 60°C for 20min, heat up to 95°C and add 30U of α-amylase (Enzyme activity 2000U / mL, Shanghai Aladdin Biochemical Technology Co., Ltd.) was liquefied for 60 minutes, then cooled to 65°C and 150 U of glucoamylase (enzyme activity 10000U / mL, Shanghai Aladdin Biochemical Technology Co., Ltd.) was added for saccharification for 20 minutes. During the above process, glass rods are used to continuously stir to ensure uniform mass transfer and fully liquefy and saccharify the cassava flour. After cooling, add 0.2 g of ammonium sulfate (Guangzhou Chemical Reagent Factory), 0.5 g of yeast extract powder (Qingdao Hi-tech Park Haibo Biotechnol...

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Abstract

The invention discloses a reversible regulation method for yeast flocculation and applications of the reversible regulation method. The reversible regulation method comprises the following steps: culturing a non-self-flocculating yeast by adopting a seed medium, thus obtaining a yeast seed solution; inoculating the yeast seed solution to a fermentation medium for fermentation, thus obtaining fermented mash; adding a flocculating-promoting agent into the fermented mash, carrying out standing for settling till layering occurs, and separating the upper layer from the lower layer, wherein the lower layer is yeast emulsion; adding a deflucculating agent into the yeast emulsion, carrying out shaking till the uniform state is achieved, inoculating the solution to a new fermentation medium for fermentation, thus obtaining fermented mash, and completing the operation; or repeating the flocculation-promoting step or the deflocculation step, so that the yeast cells are recycled. According to the method and the applications, the adopted flocculating-promoting agent and the deflucculating agent are safe and non-toxic, and serve as yeast nutrients, and in addition, the flocculating-promoting agent only selectively induces the yeast flocculation but has no flocculation effect for other particle impurities. With the adoption of the method provided by the invention for recycling the yeast, not only can the recycling separating energy consumption be saved, but also culture materials can be saved, the wine yield can be increased, and the purpose of energy conservation and emission reduction is realized systematically.

Description

technical field [0001] The invention relates to the technical field of industrial microbial alcohol fermentation, in particular to a reversible control method and application of yeast flocculation. Background technique [0002] As an important industrial basic raw material, alcohol has been widely used in food, medicine, chemical industry and other fields. In recent years, with the depletion of petroleum, coal and other fossil energy sources and the increasing pollution of the atmospheric environment by combustion waste gas, alcohol, as a renewable and clean energy source, has attracted more and more attention from all over the world. At present, my country's annual alcohol production ranks third in the world, 96.5% of which are produced by microbial fermentation. Non-grain fermentation materials such as cassava, sugarcane molasses, and sweet sorghum are popular, but there are still many problems that need to be solved in the alcohol fermentation industry, such as High energ...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12P7/06C12R1/865
CPCC12P1/02C12P7/06C12N1/185C12R2001/865Y02E50/10
Inventor 徐日益黄向阳尚红岩梁磊苏江滨郭艺山高亚飞
Owner 广东省科学院生物与医学工程研究所