A kind of hybrid paulownia seedling cultivation method
A cultivation method and technology of paulownia, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of large area, long production cycle of seedlings, difficulty in realizing large-scale industrialized planting and cultivation, etc., and achieve low environmental pollution. , increased growth rate and planting benefits, highly uniform effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0033] A kind of hybrid paulownia seedling cultivating method of the present invention comprises the steps:
[0034] S1. Carry out aseptic bud culture: take hybrid paulownia paulownia tender stems or tender shoots as explants, first sterilize with 70-80% alcohol by volume ratio, then wash once with sterile water, and then adopt a mass fraction of 0.05-0.15 %HgCl 2 After the solution is sterilized, it is washed with sterile water for 3-5 times, and then cultured on a sterile bud culture medium, and the sterile buds are obtained after 16-18 days of cultivation.
[0035] The number of days of cultivation is mainly determined by the growth rate of the hybrid paulownia buds, and it is generally advisable for the aseptic buds to grow to about 1 cm. Of course, if the sterile sprout is longer than 1 centimeter (for example, grows to 1.5 centimeters) or shorter than 1 centimeter (for example, grows to 0.5 centimeters), it does not affect the realization of the purpose of the present i...
Embodiment 2
[0067] A kind of hybrid paulownia seedling cultivating method of the present invention comprises the steps:
[0068] S1. Carry out aseptic bud culture: take hybrid paulownia paulownia tender stems or tender shoots as explants, first immerse and disinfect in 75% alcohol by volume ratio, then wash once with sterile water, and then use 0.15% HgCl in mass fraction 2 After the solution is sterilized, it is washed with sterile water for 3-5 times, and then cultured on a sterile bud culture medium, and the sterile buds are obtained after 16-18 days of cultivation.
[0069] The number of days of cultivation is mainly determined by the growth rate of the hybrid paulownia buds, and it is generally advisable for the aseptic buds to grow to about 1 cm. Of course, if the sterile sprout is longer than 1 centimeter (for example, grows to 1.5 centimeters) or shorter than 1 centimeter (for example, grows to 0.5 centimeters), it does not affect the realization of the purpose of the present inve...
Embodiment 3
[0101] A kind of hybrid paulownia seedling cultivating method of the present invention comprises the steps:
[0102] S1, carry out aseptic bud culture:
[0103] Put the hybrid Paulownia that has just grown buds into a biochemical incubator, and after cultivating at 38-40°C for 6-8 days, cut the hybrid Paulownia buds and soak them in alcohol with a volume ratio of 70-80% for 1 minute to sterilize and mass ratio 0.05 -0.15% HgCl 2After the solution is sterilized, after washing with sterile water for 3-5 times, cut out the 1.8mm long bud tip and put it into the culture medium. Cultivate and repeat this for three or more times to obtain detoxified aseptic buds; because certain viruses can be killed after high-temperature cultivation, the conduction tissue at the growth point of the 2mm-long bud tip has not yet developed completely, which is not conducive to virus conduction, and a relatively high temperature can be obtained. Good virus-free aseptic buds, the advantage of such vi...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More