Regenerated fibroin protein gel mask

A technology for regenerating silk fibroin and silk fibroin, which is applied in the field of transparent silk fibroin gel membranes, can solve problems such as poor physical and mechanical properties and affect the application prospects of silk fibroin gels, and achieve excellent stability and good cell Effects of biocompatibility and excellent mechanical properties

Active Publication Date: 2017-01-11
尤学敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this gel material is white and opaque, the interior is mainly porous, and its physical and mechanical properties are very poor, similar to "tofu dregs". These properties affect the application prospects of silk fibroin gel

Method used

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  • Regenerated fibroin protein gel mask
  • Regenerated fibroin protein gel mask
  • Regenerated fibroin protein gel mask

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Natural mulberry silk was boiled with 0.5wt% sodium bicarbonate solution for 60 minutes to obtain degummed mulberry silk;

[0041] (2) Dissolve the degummed silk in step (1) in 8% ZnCl at a concentration of 10wt% 2 In -90% formic acid, the dissolution temperature is 25°C, and the dissolution time is 3 hours to obtain a silk fibroin solution. After drying at a temperature of 20°C and a relative humidity of 65%, a CaCl-containing solution is obtained. 2 silk fibroin film;

[0042] (3) the CaCl obtained in step (2) 2 The silk fibroin film is soaked in running water and rinsed for 5 hours to obtain a regenerated silk fibroin gel film with a water content of about 65%.

Embodiment 2

[0044] (1) Natural tussah silk was degummed by boiling 0.5wt% sodium carbonate solution for 30 minutes, and the degummed tussah silk was obtained after repeating 3 times;

[0045] (2) Dissolve the degummed silk in step (1) in 4% CaCl at a concentration of 8wt% 2 In -88% formic acid, the dissolution temperature is 35°C, and the dissolution time is 4h to obtain a silk fibroin solution. After drying at a temperature of 30°C and a relative humidity of 60%, a CaCl-containing solution is obtained. 2 silk fibroin film;

[0046] (3) the CaCl obtained in step (2) 2The silk fibroin film was immersed in 25% ethanol aqueous solution for 10 hours, and the liquid was changed every half hour, and then washed in running water for 3 hours to obtain a regenerated tussah silk fibroin gel film with a water content of about 60%.

Embodiment 3

[0048] (1) Natural mulberry silk was degummed by boiling with 0.25wt% sodium bicarbonate solution for 30 minutes, and repeated 3 times to obtain degummed mulberry silk;

[0049] (2) Dissolve the degummed silk in step (1) in 4% MgCl at a concentration of 8wt% 2 In -98% formic acid, the dissolution temperature is 35°C, and the dissolution time is 1h to obtain a silk fibroin solution. After drying at a temperature of 30°C and a relative humidity of 40%, a CaCl-containing solution is obtained. 2 silk fibroin film;

[0050] (3) the MgCl obtained in step (2) 2 The silk fibroin film was soaked in running water and rinsed for 5 hours to obtain a regenerated silk fibroin gel film with a water content of about 55%.

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Abstract

The invention discloses a regenerated fibroin protein gel mask and a preparation method thereof; the preparation method comprises: (1) dissolving degummed silk directly into salt-bearing formic acid solution, and drying to obtain a salt-bearing fibroin protein mask; (2) soaking the dried mask directly in alcohol solution or water to obtain the regenerated fibroin protein gel mask; compared with the prior art, the gel mask has the advantages of both traditional fibroin protein mask and hydrogel, has excellent transparency and mechanical properties, has high water content, also has bionic nanofiber structure, good degradability and good biocompatibility; the preparation method of the gel mask is simple, has a short process and is easy to produce in batch; the gel mask has excellent physicochemical properties, biological characteristics such hat the gel mask has a promising application prospect in the field of cosmetics and regenerative medicine, such as facial mask, skin dressing, cerebral dura mater, cornea, anti-adhesion film, nerve sheaths and the like.

Description

[0001] The present invention is a divisional application for an invention patent with the application number CN201410762084.2, the application date is December 12, 2014, and the invention name is "a regenerated silk fibroin gel film and its preparation method". technical field [0002] The invention relates to a silk fibroin gel material, in particular to a transparent silk fibroin gel film, which can be widely used in the fields of biology and medicine. Background technique [0003] my country is the main producer of silk, and its silk output accounts for more than 70% of the world's output. In recent years, the research and application of silk has extended from the traditional textile field to high-tech fields, such as optoelectronics and biomedical materials, especially as biomedical materials have made important progress. Silk fibroin is obtained by degumming silk. It is a natural structural protein without physiological activity. It is mainly composed of three simple am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/42A61L15/62A61L26/00A61L27/22A61L27/52A61L27/50A61L31/04A61L31/14A61K47/42A61K9/00A61K8/64
CPCA61K8/64A61K9/0002A61K47/42A61L15/32A61L15/42A61L15/62A61L26/0047A61L26/0061A61L27/227A61L27/50A61L27/52A61L31/047A61L31/14A61L31/145A61L2430/16A61L2430/32C08L89/00
Inventor 张锋别诗宇周燕左保齐
Owner 尤学敏
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