A method for producing trichoderma bio-organic fertilizer by using green onion leaf waste
A bio-organic fertilizer and waste technology, applied in the field of agricultural waste reuse, to achieve good growth promotion effect, prevent and control plant pathogenic fungi, and improve soil
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Embodiment 1
[0033] Activation of Trichoderma viride LTR-2 strain and preparation of seed solution
[0034] Trichoderma viride ( Trichoderma Viride ) LTR-2, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, its preservation number is CGMCC No.1498, and the preservation date is October 20, 2005. It has been disclosed on June 14, 2006 in the patent application whose publication number is CN1786150A.
[0035] Inoculate the LTR-2 strain stored at -70°C on PDA solid medium, and after culturing at 28°C for 2 days, pick fresh bacterial blocks and inoculate them into sterilized potato glucose liquid medium, and culture at 28°C with shaking at 150-180 rpm After 3-5 days, it will be used as a liquid strain.
Embodiment 2
[0037] Trichoderma viridans cultured from green onion leaf waste
[0038] Mix the dry and crushed scallion green onion leaves and bran in a certain proportion, adjust the water content, pack them in edible fungus culture bags, sterilize at 121°C for 25 minutes, inoculate liquid strains, and culture them at about 28°C for 10 days. Counting method was used to detect the number of conidia of LTR-2 in the fermentation material. The influences of green onion leaf content, pH value, water content and other factors on the cultivation of Trichoderma LTR-2 from green onion leaf waste were studied respectively. For details, please refer to Table 1 and the accompanying drawings figure 1 with figure 2 shown.
[0039] Table 1 Effects of different ratios of green onion leaves and bran on sporulation of Trichoderma LTR-2
[0040] .
[0041] It can be seen from Table 1 that the ratio of green onion leaves to bran is 2:3-3:3, and a higher number of conidia can be obtained at the end o...
Embodiment 3
[0043] Preparation of Trichoderma Biological Organic Fertilizer
[0044]The carrier used in Trichoderma bio-organic fertilizer is compost after aerobic fermentation of industrial and agricultural solid wastes such as sugar residue, starch residue, straw, chicken manure, etc., fully decomposed. After decomposing, the compost temperature drops below 40°C, check whether the various indicators of the compost meet the national requirements, and then inoculate about 1% of the conidia culture of Trichoderma onifolia (the number of conidia is 30×10 8 cfu / g), after mixing evenly, dry with warm air at 40-50°C until the moisture content is about 15-20%, that is, the conidia containing 0.2×10 8 Trichoderma bio-organic fertilizer above cfu / g. The number of Trichoderma spores in Trichoderma bio-organic fertilizer is greater than 2×10 7 Individual / g, total nitrogen, phosphorus and potassium nutrients ≥ 5%, organic matter mass ratio content ≥ 45%.
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