A method for producing trichoderma bio-organic fertilizer by using green onion leaf waste

A bio-organic fertilizer and waste technology, applied in the field of agricultural waste reuse, to achieve good growth promotion effect, prevent and control plant pathogenic fungi, and improve soil

Active Publication Date: 2019-09-10
ECOLOGY INST SHANDONG ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solid fermentation technology is currently recognized as the most effective method for obtaining conidia. The production of Trichoderma conidia reported so far mostly uses grains such as wheat and corn and their by-products as the main components of the medium, and some use waste water to produce preparations. , but the method for producing Trichoderma conidia with green onion leaves has not been reported yet

Method used

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  • A method for producing trichoderma bio-organic fertilizer by using green onion leaf waste
  • A method for producing trichoderma bio-organic fertilizer by using green onion leaf waste

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Experimental program
Comparison scheme
Effect test

Embodiment １

[0033] Activation of Trichoderma viride LTR-2 strain and preparation of seed solution

[0034] Trichoderma viride ( Trichoderma Viride ) LTR-2, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, its preservation number is CGMCC No.1498, and the preservation date is October 20, 2005. It has been disclosed on June 14, 2006 in the patent application whose publication number is CN1786150A.

[0035] Inoculate the LTR-2 strain stored at -70°C on PDA solid medium, and after culturing at 28°C for 2 days, pick fresh bacterial blocks and inoculate them into sterilized potato glucose liquid medium, and culture at 28°C with shaking at 150-180 rpm After 3-5 days, it will be used as a liquid strain.

Embodiment 2

[0037] Trichoderma viridans cultured from green onion leaf waste

[0038] Mix the dry and crushed scallion green onion leaves and bran in a certain proportion, adjust the water content, pack them in edible fungus culture bags, sterilize at 121°C for 25 minutes, inoculate liquid strains, and culture them at about 28°C for 10 days. Counting method was used to detect the number of conidia of LTR-2 in the fermentation material. The influences of green onion leaf content, pH value, water content and other factors on the cultivation of Trichoderma LTR-2 from green onion leaf waste were studied respectively. For details, please refer to Table 1 and the accompanying drawings figure 1 with figure 2 shown.

[0039] Table 1 Effects of different ratios of green onion leaves and bran on sporulation of Trichoderma LTR-2

[0040] .

[0041] It can be seen from Table 1 that the ratio of green onion leaves to bran is 2:3-3:3, and a higher number of conidia can be obtained at the end o...

Embodiment 3

[0043] Preparation of Trichoderma Biological Organic Fertilizer

[0044]The carrier used in Trichoderma bio-organic fertilizer is compost after aerobic fermentation of industrial and agricultural solid wastes such as sugar residue, starch residue, straw, chicken manure, etc., fully decomposed. After decomposing, the compost temperature drops below 40°C, check whether the various indicators of the compost meet the national requirements, and then inoculate about 1% of the conidia culture of Trichoderma onifolia (the number of conidia is 30×10 8 cfu / g), after mixing evenly, dry with warm air at 40-50°C until the moisture content is about 15-20%, that is, the conidia containing 0.2×10 8 Trichoderma bio-organic fertilizer above cfu / g. The number of Trichoderma spores in Trichoderma bio-organic fertilizer is greater than 2×10 7 Individual / g, total nitrogen, phosphorus and potassium nutrients ≥ 5%, organic matter mass ratio content ≥ 45%.

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Abstract

The invention discloses a method for producing a trichoderma bio-organic fertilizer by using a green Chinese onion leaf waste. The green Chinese onion leaf waste is taken as a main ingredient of a culture medium, a trichoderma is inoculated for solid fermentation so as to acquire a large quantity of conidiums, and then a thoroughly decomposed organic fertilizer is added to produce the trichoderma bio-organic fertilizer with a bacteriostasis function. According to the technical scheme, a novel way is provided for the resource utilization of the green Chinese onion leaf waste, and a produced Trichoderma antibiological inoculant is applied in the field so as to exert the multiple effects of preventing plant pathogenic fungi, promoting the crop growth and improving the soil.

Description

technical field [0001] The invention relates to the technical field of agricultural waste reuse, in particular to a method for producing Trichoderma bio-organic fertilizer by using green onion leaf waste. Background technique [0002] Scallions, pungent in taste, slightly warm in nature, have the functions of promoting yang, detoxifying seasoning, sweating, inhibiting bacteria and relaxing blood vessels. In East Asian countries and various Chinese regions, onion is often eaten as a very common spice or vegetable, and plays an important role in oriental cooking. [0003] Wastes such as green onion leaves produced during the production and processing of green onions account for 30% of the total output of green onions. Because these green onion wastes are randomly dumped and piled up in rural fields, ditches, and roadsides, the utilization conversion rate is low, the harmless treatment is not in place, and the rot and deterioration have caused serious environmental pollution, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C12N1/14C12R1/885
Inventor 魏艳丽李纪顺扈进冬李红梅刘伟陈凯王贻莲
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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