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Electrochemical testing strip and method for simultaneously testing various substances to be tested

An electrochemical and test strip technology, applied in the field of electrochemical detection, can solve the problems of troublesome operation, time-consuming, and reducing the number of working electrodes for users

Active Publication Date: 2017-01-25
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above measurement process, due to the need to inhale blood multiple times and replace different electrochemical test strips, the user's operation is troublesome, time-consuming, and increases the cost of using the test strips
[0004] Although some products can detect multiple objects to be tested, they usually divide different detection areas on the same test strip, and each area is independently equipped with a fixed reference / counter electrode paired with the corresponding working electrode to form a measurement The main function of the reference / counter electrode is to maintain the working electrode potential and current loop. This measurement mode objectively reduces the number of working electrodes that can detect the target in the sample, increases the number of printed electrodes, and increases the production cost of the electrode.

Method used

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  • Electrochemical testing strip and method for simultaneously testing various substances to be tested
  • Electrochemical testing strip and method for simultaneously testing various substances to be tested
  • Electrochemical testing strip and method for simultaneously testing various substances to be tested

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] The schematic diagram of the electrode type test strip is shown in figure 1 As shown, the schematic diagram of its structural decomposition is shown in figure 2 shown.

[0128] figure 1 , figure 2 It is shown that the electrode-type test strip provided by the present invention includes a plate body 100 and an electrode located inside the plate body 100; one end of the plate body 100 is a collection terminal 20, and the other end is an electrical contact terminal 10, which can be inserted into the current Response analysis device;

[0129] The plywood body 100 includes multiple layers of insulating material stacked in sequence, from bottom to top are the first base layer 200, the second base layer 300, the first upper layer 400 and the second upper layer 500;

[0130] The surface of the first base layer 200 in contact with the second base layer 300 is provided with electrodes, and the electrodes include a first working electrode 11, a reference electrode 12 and a s...

Embodiment 2

[0136] The modified enzyme membranes for detecting blood sugar, blood ketone and ethanol were respectively coated on the three electrodes of the electrode test strip prepared in Example 1. Coated reagents are shown in Tables 1-3:

[0137] Table 1. Blood ketone sensor working electrode enzyme membrane

[0138] components content enzyme beta-hydroxybutyrate dehydrogenase 1％ coenzyme NAD +

3％ Adhesion promoter Hydroxyethyl cellulose 5％ Surfactant Triton-100 0.2％ electron mediator Azure I 3％ filler lactose 5％ solvent Phosphate buffer 82.8％

[0139] Coat the modified enzyme membrane of blood ketones on the surface of the electrode 11, the method is: drop 0.15 microliters of the enzyme membrane reaction solution in Table 1, and dry at 37° C. for 4 minutes.

[0140] Table 2. Glucose sensor working electrode enzyme membrane

[0141] components content enzyme glucose oxidase 1％ bi...

Embodiment 3

[0147] The modified enzyme membranes for detecting total cholesterol, triglyceride and uric acid were respectively coated on the three electrodes of the electrode-type test strip prepared in Example 1. Coated reagents are shown in Tables 4-6:

[0148] Table 4 Total cholesterol sensor working electrode enzyme membrane

[0149] components content enzyme cholesterol esterase 2％ enzyme cholesterol oxidase 4％ enzyme Peroxidase 1％ Adhesion promoter Hydroxyethyl cellulose 5％ Surfactant Triton-100 0.2％ electron mediator Potassium ferrocyanide 3％ filler lactose 5％ solvent water 79.8％

[0150] Coat the modified enzyme membrane of total cholesterol on the surface of the electrode 11 by adding dropwise 0.15 μl of the enzyme membrane reaction solution in Table 4, and drying at 37° C. for 4 minutes.

[0151] Table 5 Uric acid sensor working electrode enzyme membrane

[0152] components content ...

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Abstract

The invention relates to the technical field of electrochemical testing, and in particular to an electrochemical testing strip and a method for simultaneously testing various substances to be tested. All electrodes in the electrochemical testing strip provided by the invention are coated with modification enzyme membranes, and each electrode can be used as a working electrode for testing a specific target analysis substance in blood. The occupancy rate of the working electrodes in the electrochemical testing strip is improved, the capability of the electrochemical testing strip for testing a multi-component target substance is improved, the electrode production cost is reduced, the operation steps of testing multiple target analysis substances in the same sample are simplified, the use cost is reduced, and the amount of the required samples is reduced. The experiment shows that the electrochemical testing strip provided by the invention is used for testing the substances to be tested in the blood, so that the linearity range is wider, the recovery rate is 93 percent to 103 percent, and CV test performance is stable.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to an electrochemical test strip and a method for simultaneously detecting multiple analytes. Background technique [0002] Electrochemical test strips based on the principle of electrochemical biosensors are widely used in the field of point-of-care testing (POCT). The electrochemical test strip selectively recognizes the target analyte in the sample to be tested by modifying the biomolecules (such as enzymes) on the electrode surface, and converts the biorecognition signal into an electrical signal (oxidation or reduction current) that can be collected and measured. Achieve quantitative detection of target analytes. Generally, electrochemical test strips are mainly used for the detection of multiple analytes in blood, and the detectable substances include blood sugar, blood ketones, uric acid, creatinine, total cholesterol, triglycerides, high-density lipoprotei...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/327
CPCG01N27/3272G01N27/416
Inventor 黄钊蔡晓华
Owner SINOCARE
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