Electrochemical testing strip and method for simultaneously testing various substances to be tested
An electrochemical and test strip technology, applied in the field of electrochemical detection, can solve the problems of troublesome operation, time-consuming, and reducing the number of working electrodes for users
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[0126] Example 1
[0127] The schematic diagram of the electrode test strip is as follows figure 1 As shown, its structural decomposition diagram is as follows figure 2 shown.
[0128] figure 1 , figure 2 It is shown that the electrode-type test strip provided by the present invention includes a board body 100 and an electrode located inside the board body 100; one end of the board body 100 is the collection end 20, and the other end is the electrical contact end 10, and the electrical contact end 10 can be inserted into the current response analysis device;
[0129] The board layer body 100 includes multiple layers of insulating materials stacked in sequence, from bottom to top, the first base layer 200 , the second base layer 300 , the first upper layer 400 and the second upper layer 500 ;
[0130] The surface of the first base layer 200 in contact with the second base layer 300 is provided with electrodes, and the electrodes include a first working electrode 11 , a r...
Example Embodiment
[0135] Example 2
[0136] Modified enzyme membranes for detecting blood sugar, blood ketones and ethanol were respectively coated on the three electrodes of the electrode-type test strip prepared in Example 1. The coated reagents are shown in Tables 1-3:
[0137] Table 1. Blood Ketone Sensor Working Electrode Enzyme Membrane
[0138] component content enzyme β-hydroxybutyrate dehydrogenase 1% coenzyme NAD +
[0139] The modified enzyme membrane of blood ketone was coated on the surface of the electrode 11 by dropwise adding 0.15 μl of the enzyme membrane reaction solution of Table 1, and drying at 37° C. for 4 min.
[0140] Table 2. Glucose Sensor Working Electrode Enzyme Membrane
[0141] component content enzyme glucose oxidase 1% binder Hydroxyethyl cellulose 5% Surfactant Triton-100 0.2% electron mediator Potassium ferricyanide 3% filler lactose 5% solvent Phosphate buffer 85.8% ...
Example Embodiment
[0146] Example 3
[0147] Modified enzyme membranes for detecting total cholesterol, triglyceride and uric acid were respectively coated on the three electrodes of the electrode test strip prepared in Example 1. The coated reagents are shown in Tables 4-6:
[0148] Table 4 Total cholesterol sensor working electrode enzyme membrane
[0149] component content enzyme cholesterol esterase 2% enzyme cholesterol oxidase 4% enzyme peroxidase 1% Adhesion promoter Hydroxyethyl cellulose 5% Surfactant Triton-100 0.2% electron mediator Potassium ferrocyanide 3% filler lactose 5% solvent water 79.8%
[0150] The modified enzyme membrane of total cholesterol was coated on the surface of the electrode 11 by dropwise adding 0.15 μl of the enzyme membrane reaction solution of Table 4, and drying at 37° C. for 4 min.
[0151] Table 5 Uric acid sensor working electrode enzyme membrane
[0152] compo...
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