Carbofuran monoclonal antibody hybridoma cell strain YH1 and application thereof

A hybridoma cell line, monoclonal antibody technology, applied in biochemical equipment and methods, instruments, microorganisms, etc., can solve the problems of high cost, high toxicity of carbocarb, and expensive equipment operators.

Active Publication Date: 2017-02-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high toxicity of carbofuran, it is not easy to degrade in acidic soil, and it is easy to pollute soil and groundwater sources, especially when used in large quantities on food and vegetables, forming pesticide residues, causing immeasurable harm to public health
[0003] At present, the detection of carbofuran chromatography is the most widely used, including gas chromatography (GC), high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry

Method used

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  • Carbofuran monoclonal antibody hybridoma cell strain YH1 and application thereof
  • Carbofuran monoclonal antibody hybridoma cell strain YH1 and application thereof
  • Carbofuran monoclonal antibody hybridoma cell strain YH1 and application thereof

Examples

Experimental program
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Embodiment 1

[0027] Example 1 Preparation of Carbofuran Monoclonal Antibody Hybridoma Cell Line YH1

[0028] (1) Synthesis of hapten: Dissolve furanol (1.64 g, 10 mmol) in dichloromethane (50 mL), add triethylamine (1.11 g, 11 mmol) under ice-cooling, stir for 10 min, then add the corresponding Di(p-nitrophenyl)carbonate (3.19 g, 10.5 mmol), gradually warmed up to room temperature (30°C), stirred for 1 h, and the reaction was completed by TLC plate detection (Rf = 0.6, petroleum ether: ethyl acetate = 10:1) ,stand-by. Under ice-cooling, a dichloromethane solution (10 mL) of 4-aminobutyric acid methyl ester hydrochloride (1.697 g, 10 mmol) basified with triethylamine (2.22 g, 22 mmol) was added to the above reaction solution, Stir overnight at room temperature, wash with water, wash with saturated brine (50 mL*2 each), dry over anhydrous sodium sulfate, filter to remove the desiccant, spin the filtrate to dryness in vacuo, and purify by column chromatography (silica gel (200-300 mesh), mob...

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Abstract

Provided is a Carbofuran monoclonal antibody hybridoma cell strain YH1 and an application thereof, which belongs to the field of food safety immunological detection. The Carbofuran monoclonal antibody hybridoma cell strain YH1 has been preserved in China General Microbiological Culture Collection Center, CGMCC for short, and the preservation number is CGMCC No. 12029. The monoclonal antibody secreted by the cell strain has better specificity and detection sensitivity on carbofuran, and IC50 value is 0.3 ng/mL, thus achieving detections of residual amounts of carbofuran in water, fruits and vegetables and cereals, providing conditions for immunodetection of residual carbofuran in food and having practical application value.

Description

technical field [0001] The invention relates to a carbofuran monoclonal antibody hybridoma cell line YH1 and application thereof, belonging to the field of food safety immunology detection. Background technique [0002] Carbofuran, also known as carbofuran, is a carbamate insecticide, acaricide, and nematicide. It was developed and produced by FMC Corporation and Mobay Chemical Corporation in 1969. It is widely used in the control of pests in grain, vegetables, fruits and cash crops. According to China's pesticide toxicity classification standards, carbofuran is a highly toxic pesticide. Birds or other insects poisoned by furan can cause secondary poisoning and death after being foraged by raptors, small animals or reptiles. Due to the high toxicity of carbofuran, it is not easy to degrade in acidic soil, and it is very easy to pollute soil and groundwater sources, especially when used in large quantities on food and vegetables, forming pesticide residues and causing immeas...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/44G01N33/577C12R1/91
CPCC07K16/44G01N33/577
Inventor 胥传来姚蕾珺匡华徐丽广马伟刘丽强吴晓玲宋珊珊胡拥明
Owner JIANGNAN UNIV
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