A method for whole-cell biocatalytic production of glutaric acid

A technology of biocatalysis and glutaric acid, applied in the field of biology, can solve problems such as time-consuming, increased production costs, and difficult product separation
CN106367445BActive Publication Date: 2019-08-20NANJING TECH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING TECH UNIV
Publication Date
2019-08-20

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Abstract

The invention discloses a method of whole-cell-biocatalytically producing glutaric acid. The method includes the steps of: performing induced expression and collecting cells of a recombinant strain E.coli BL-22AB-YDT and a recombinant strain E.coli 28LGOX, and mixing the cells of the recombinant strain E.coli 28LGOX and the recombinant strain E.coli BL-22AB-YDT according to the ratio of 1:1-5; adding substrates including L-glutamic acid and L-lysine according to the molar ratio of 1:0.5-4; and adding a surfactant to perform the whole-cell-catalytic production to obtain the glutaric acid. The method is free of addition of 2-ketoglutaric acid, so that production cost is reduced, and problems of long production period, complex metabolic products, low substrate conversion rate, difficulty in separation and extraction of products, and high energy consumption in a fermentation method are solved. The method also solves a defect that a cascade catalytic process in enzymic catalysis is difficult to achieve, so that the method can improve catalytic efficiency and is free of an enzyme purifying process.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for producing glutaric acid by whole cell biocatalysis. Background technique

[0002] Glutaric acid, alias: gum acid, α,γ-propanedicarboxylic acid, 1,3-propanedicarboxylic acid, can be used as an important chemical raw material and organic intermediate, and has a wide range of applications in various fields. In industry, glutaric acid can be used to synthesize polyvinyl chloride, polyester, polyamide and polyester plasticizer of polyvinyl chloride, etc. It can also be used to synthesize liquid polyester (improving the molecular structure of PET fiber, improving PET Fiber dyeability, improve dye uptake rate). In medicine, glutaric acid can be used to synthesize various sterilization lotions and medicines. In addition, in daily life, glutaric acid can be used in the formulation of detergents, the preparation of adhesives, and the preparation of sulfur-containing flue gases. ...

Claims

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