Method for screening and enrichment culture of nitrifying bacterium floras with alternation of intermittent ammonia nitrogen flow and intermittent operation
A nitrifying bacteria, enrichment culture technology, applied in the direction of chemical instruments and methods, biochemical equipment and methods, microbial-based methods, etc., can solve the problems of reduced reaction rate, difficulty in maintaining short-term nitrification, slow nitrification reaction rate, etc.
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[0082] 1. Daytime intermittent operation cycle (A) to cultivate the nitrifying bacteria flora
[0083] Take sewage treatment plant A 2 / O process reflux sludge is inoculated sludge, and the automatic bacterial fermentation tank is used as the test device, with an effective volume of 6L, such as figure 1 shown. The sludge concentration (MLSS) of the inoculum sludge was 4573mg / L. Temperature 23±1°C, pH7.2~7.4, reaction initial DO1.0~1.5mg / L, stirring speed 100rpm, Na 2 CO 3 The stock solution concentration is 200g / L. The nitrifying bacterial flora was cultivated by intermittent, ammonia nitrogen feeding-intermittent operation alternately. The reactor was operated for 25 days, and the first 24 days were intermittent and ammonia nitrogen feeding-intermittent each cycle. The composition and concentration of the basal culture medium are shown in Table 1:
[0084] Table 1 basal culture solution components (the following components are not mixtures, but multiple separate solutio...
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