Method for screening and enrichment culture of nitrifying bacterium floras with alternation of intermittent ammonia nitrogen flow and intermittent operation

A nitrifying bacteria, enrichment culture technology, applied in the direction of chemical instruments and methods, biochemical equipment and methods, microbial-based methods, etc., can solve the problems of reduced reaction rate, difficulty in maintaining short-term nitrification, slow nitrification reaction rate, etc.

Active Publication Date: 2017-02-15
BEIJING UNIV OF TECH
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  • Abstract
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Problems solved by technology

[0004] Sewage treatment plants generally use A 2 / O process for biological denitrification. In practice, due to limited operating conditions, it is difficult to maintain short-term nitrification, and the whole process of nitrification is generally used.
The nitrification reaction rate of the existing process is relatively slow, and the reaction rate drops significantly in winter due to the decrease of water temperature, so upgrading the technology has extremely important economic value

Method used

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  • Method for screening and enrichment culture of nitrifying bacterium floras with alternation of intermittent ammonia nitrogen flow and intermittent operation
  • Method for screening and enrichment culture of nitrifying bacterium floras with alternation of intermittent ammonia nitrogen flow and intermittent operation
  • Method for screening and enrichment culture of nitrifying bacterium floras with alternation of intermittent ammonia nitrogen flow and intermittent operation

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Experimental program
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Effect test

Embodiment 1

[0082] 1. Daytime intermittent operation cycle (A) to cultivate the nitrifying bacteria flora

[0083] Take sewage treatment plant A 2 / O process reflux sludge is inoculated sludge, and the automatic bacterial fermentation tank is used as the test device, with an effective volume of 6L, such as figure 1 shown. The sludge concentration (MLSS) of the inoculum sludge was 4573mg / L. Temperature 23±1°C, pH7.2~7.4, reaction initial DO1.0~1.5mg / L, stirring speed 100rpm, Na 2 CO 3 The stock solution concentration is 200g / L. The nitrifying bacterial flora was cultivated by intermittent, ammonia nitrogen feeding-intermittent operation alternately. The reactor was operated for 25 days, and the first 24 days were intermittent and ammonia nitrogen feeding-intermittent each cycle. The composition and concentration of the basal culture medium are shown in Table 1:

[0084] Table 1 basal culture solution components (the following components are not mixtures, but multiple separate solutio...

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Abstract

The invention discloses a method for screening and enrichment culture of nitrifying bacterium floras with alternation of intermittent ammonia nitrogen flow and intermittent operation, and belongs to the technical field of water treatment. According to the method, activated sludge containing nitrifying bacteria is used as inoculation sludge, and the screening and enrichment culture of the nitrifying bacterium floras are achieved by using a bacterial fermentation tank by virtue of a method for alternation of intermittent ammonia nitrogen flow and intermittent operation. The initial ammonia nitrogen load is gradually increased by using intermittent operation so as to improve the ammonia-nitrogen oxidation rate, and the ammonia nitrogen flow and intermittent operation maintain the ammonia-nitrogen oxidation rate and achieve a low nitrite accumulation rate. By adopting the method, the screening and enrichment culture of the nitrifying bacterium floras can be achieved in a short time. The nitrifying bacterium floras obtained by the method disclosed by the invention have an advantage in numbers in total bacterial count, and a high ammonia-nitrogen oxidation rate and a high nitrite oxidation rate can be achieved.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to a method for screening and enriching culture of nitrifying bacteria flora in batch mode, ammonia nitrogen feeding-intermittent operation alternately. Background technique [0002] A large amount of ammonia nitrogen wastewater discharge can cause water eutrophication, the main source of which is domestic sewage, industrial wastewater and agricultural water. With the increasingly serious trend of water eutrophication at home and abroad and the relevant departments attach great importance to this issue, reducing external sources has become the primary measure to control water eutrophication. For this reason, a large number of sewage treatment plants have been built to treat sewage through specific processes. Effective treatment reduces the input of pollutants, and the effluent standards of sewage treatment plants are becoming stricter. Sewage treatment plants in my countr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/02C12N1/02C12N1/20C12N1/36C02F101/16C02F103/20C12R1/01
CPCC02F3/02C02F3/34C02F2101/16C02F2101/166C02F2103/20C02F2209/14C02F2209/15C02F2305/06C12N1/02C12N1/20C12N1/36Y02W10/10
Inventor 杨宏姚仁达
Owner BEIJING UNIV OF TECH
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