O type foot-and-mouth disease virus artificial recombinant antigen and preparation and application thereof

A foot-and-mouth disease virus and recombinant antigen technology, which is applied in the field of biological preparations, can solve the problems of false positives and missed detection, and achieve the effects of high specificity and sensitivity, easy preparation and operation, and reduced missed detection rate

Active Publication Date: 2017-02-15
北京世纪元亨动物防疫技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Tian Kegong, Sun Ming and others conducted related research on 3B in 2007 and found that the specificity and sensitivity of 3B antigen in the differential diagnosis of healthy cattle, immunized cattle and non-immunized infected cattle are good, but there are still some missed or false positive

Method used

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  • O type foot-and-mouth disease virus artificial recombinant antigen and preparation and application thereof
  • O type foot-and-mouth disease virus artificial recombinant antigen and preparation and application thereof
  • O type foot-and-mouth disease virus artificial recombinant antigen and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0065] The O-type foot-and-mouth disease live vaccine used in Example 2 was purchased from Qianyuanhao Biology Co., Ltd. Cattle O-type FMD virus standard positive serum, guinea pig type A FMD virus standard positive serum, guinea pig type C FMD virus standard positive serum, guinea pig Asia I type FMD virus standard positive serum were donated by Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. Porcine vesicular disease standard positive serum and bovine viral diarrhea standard positive serum are kept by the Veterinary Diagnostic Center of the Ministry of Agriculture. The positive serum of cattle infected with O-type FMD wild strain was prepared and stored by the Veterinary Diagnostic Center of the Ministry of Agriculture. The positive serum of O-type FMD vaccine strain immunized cattle and the serum of cattle artificially infected with FMD virus were prepared and preserved by Inner Mongolia Biopharmaceutical Factory and the Veterinary Diagnostic...

Embodiment 4

[0066] 10 parts of foot-and-mouth disease negative group bovine serums used in embodiment 4 are provided by Beijing Entry-Exit Inspection and Quarantine Bureau; each 20 parts of foot-and-mouth disease inactivated vaccine immune group bovine serums and foot-and-mouth disease virus artificially infected bovine serums are provided by Inner Mongolia Biopharmaceutical Factory and Ministry of Agriculture veterinarians Jointly prepared and preserved by the diagnostic center; 10 serums from bovines naturally infected with foot-and-mouth disease virus were prepared and preserved by the Veterinary Diagnostic Center of the Ministry of Agriculture;

Embodiment 5

[0067] O-type foot-and-mouth disease natural infection positive serum (20 parts), O-type foot-and-mouth disease artificial infection positive serum (20 parts), foot-and-mouth disease inactivated vaccine immune positive serum, vaccine immune challenge group positive serum (10 parts) and Foot-and-mouth disease negative serum (20 copies) was provided by the Veterinary Diagnostic Center of the Ministry of Agriculture.

[0068] 2. Vector and strain:

[0069] The pGEX-T-Easy vector was purchased from Promega. Dh5a competent cells were purchased from Beijing Quanshijin Biotechnology Co., Ltd. The pGEX-20T vector and the E.coli ER2556 strain were kindly donated by Professor Xia Ningshao from the Tumor and Cell Engineering Laboratory of Xiamen University.

[0070] main reagent

[0071] The total RNA extraction kit is a product of Qiagen. AMV, Rnasin, DNTPs, Taq DNA polymerase, T4 ligase, and pGEM-T-Easy were all purchased from Promega, the gel recovery kit and plasmid extraction ...

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Abstract

The invention discloses O type foot-and-mouth disease virus artificial recombinant antigen and preparation and application thereof and belongs to the field of biological preparations; an amino acid sequence of the artificial recombinant antigen comprises peptide segments shown as in Seq ID No. 4; the amino acid sequence of the artificial recombinant antigen is shown specifically as in Seq ID No. 5. The invention also claims a gene fragment to encode the artificial recombinant antigen, as well as a recombinant expression vector to express the artificial recombinant antigen and related transformants. The O type foot-and-mouth disease virus artificial recombinant antigen has the advantages such as low production cost, good safety, good antigenicity and good convenience of large-scale production, and polyclonal antibodies produced by foot-and-mouth disease virus natural infections and artificial immunity can be efficiently distinguished.

Description

technical field [0001] The invention belongs to the field of biological preparations, and in particular relates to an artificial recombinant antigen of O-type foot-and-mouth disease virus and its preparation and application. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, high-contact, and severe infectious disease of cloven-hoofed animals caused by Food and Mouth Disease Virus (FMDV). It is characterized by blisters and ulcers on the skin of the hooves and breasts. Both the World Organization for Animal Health (OIE) and the Chinese government have listed the disease as the first of Class A animal infectious diseases. Because the outbreak of the disease affects international trade and often causes huge economic losses and political influences, the prevention and control of foot-and-mouth disease has always been highly valued by governments all over the world. [0003] In the prevention and treatment of FMD, diagnosis and monitoring are cruc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/09C07K1/18C12N15/42C12N15/70C12N1/21G01N33/569
CPCC07K14/005C12N2770/32122G01N33/56983G01N2333/09
Inventor 孙明田克恭王宏伟王传彬遇秀玲陈西钊曹振刘巧荣
Owner 北京世纪元亨动物防疫技术有限公司
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