A kind of device and method for uninterrupted cultivation and production of lysine
A technology for the production of lysine and lysine, applied in biochemical equipment and methods, biological material sampling methods, biochemical cleaning devices, etc., can solve the burden, contamination, and batch-to-batch instability And other issues
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Embodiment 1
[0083] A method for continuous cultivation and production of lysine, including the following steps:
[0084] (1) Uninterrupted culture: After the first-level seed tank is cultivated to a certain concentration of bacteria, it is transferred to the second-level seed tank according to a certain amount of inoculum for cultivation, and the first-level seed tank is supplemented with a certain volume of first-level seed tank culture medium to continue the culture According to the growth time of the first-level seed tank, after reaching the required cell concentration, the second-level seed tank is inoculated, and the culture is continuously cycled. In this example, the OD of the bacteria in the first-level seed tank is maintained at 0.45, and the second-level seed tank is maintained at 0.45. The OD of the bacteria in the seed tank is 0.9;
[0085] (2) Uninterrupted fermentation: transfer the bacterial liquid obtained from the secondary seed tank to the first fermentation tank of the ferme...
Embodiment 2
[0094] A method for continuous cultivation and production of lysine, including the following steps:
[0095] (1) Uninterrupted culture: After the first-level seed tank is cultivated to a certain concentration of bacteria, it is transferred to the second-level seed tank according to a certain amount of inoculum for cultivation, and the first-level seed tank is supplemented with a certain volume of first-level seed tank culture medium to continue the culture According to the growth time of the first-level seed tank, after reaching the required cell concentration, the second-level seed tank is inoculated, and the culture is continuously cycled; in this example, the OD of the bacteria in the first-level seed tank is maintained at 0.65, and the second-level seed tank is maintained at 0.65. The OD of the bacteria in the seed tank is 1.1;
[0096] (2) Uninterrupted fermentation: transfer the bacterial liquid obtained from the secondary seed tank to the first fermentation tank of the ferme...
Embodiment 3
[0105] A method for continuous cultivation and production of lysine, including the following steps:
[0106] (1) Uninterrupted culture: After the first-level seed tank is cultivated to a certain concentration of bacteria, it is transferred to the second-level seed tank according to a certain amount of inoculum for cultivation, and the first-level seed tank is supplemented with a certain volume of first-level seed tank culture medium to continue the culture According to the growth time of the first-level seed tank, after reaching the required cell concentration, the second-level seed tank is inoculated, and the culture is continuously cycled. In this embodiment, the OD of the bacteria in the first-level seed tank is maintained at 0.5, and the second-level seed tank is maintained at 0.5 The OD of the bacteria in the seed tank is 1;
[0107] (2) Uninterrupted fermentation: transfer the bacterial liquid obtained from the secondary seed tank to the first fermentation tank of the ferment...
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