A kind of near-infrared fluorescent molecular probe and its synthesis method and application
A technology of fluorescent molecular probes and synthesis methods, applied in cell imaging, the field of preparation of molecular probes, can solve problems such as instability, leakage, low quantum yield, etc., to achieve increased conjugated systems, The effect of increasing the stock displacement and enhancing the fluorescence intensity of molecules
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0036] (1) Add 4.47mmol 2,3,3-trimethyl-3H-indoline-5-potassium sulfonate, 5.81mmol m-fluorobenzyl bromide and 12ml toluene in batches to a 50ml one-necked flask, under the protection of argon 1.31g of 1-m-fluorobenzyl-2,3,3-trimethyl-3H-indoline-5-sulfonic acid, It is a pink solid with a yield of 84.4%.
[0037] (2) Take 2.76mmol 1-m-fluorobenzyl-2,3,3-trimethyl-3H-indoline-5-sulfonic acid, 1.38mmol 2-chloro-1-formyl-3-hydroxymethylene Cyclohexene and 30ml of n-butanol: mixed solvent of toluene (v / v) = 7:3 was added in a 100ml reaction flask, under the protection of argon, heated at reflux at 75°C for 5h, the reaction was completed, and the to room temperature, concentrated under reduced pressure, then added methyl tert-butyl ether of 3 times the volume of the concentrated reaction solution, filtered, and the filter cake was separated and purified by column chromatography (silica gel column, the specification of silica gel is 200-300 mesh), and the eluent It is a mixed solu...
Embodiment 2
[0042](1) Add 4.47mmol 2,3,3-trimethyl-3H-indoline-5-potassium sulfonate, 8.94mmol m-fluorobenzyl bromide and 24ml toluene in batches to a 50ml single-necked flask, under the protection of argon at 90°C for 16 h, cooled to room temperature, filtered, washed with toluene, and dried in vacuo to obtain 1.21 g of 1-m-fluorobenzyl-2,3,3-trimethyl-3H-indoline-5-sulfonic acid, It is a pink solid.
[0043] (2) Take 2.76mmol 1-m-fluorobenzyl-2,3,3-trimethyl-3H-indoline-5-sulfonic acid, 1.38mmol 2-chloro-1-formyl-3-hydroxymethylene Cyclohexene and 20ml of pyridine were added to a 100ml reaction flask, under the protection of argon, heated to reflux at 75°C for 8h, after the reaction was completed, lowered to room temperature, concentrated under reduced pressure and added 4 times the volume of the concentrated reaction solution The methyl tert-butyl ether, filter, filter cake is separated and purified by column chromatography (silica gel column, the specification of silica gel is 200-30...
Embodiment 3
[0047] (1) Add 4.47mmol 2,3,3-trimethyl-3H-indoline-5-potassium sulfonate, 11.62mmol m-fluorobenzyl bromide and 24ml toluene in batches to a 50ml one-necked flask, under the protection of nitrogen , reacted at 90°C for 15h, lowered to room temperature, filtered, washed with toluene, and dried in vacuo to obtain 1.28g of 1-m-fluorobenzyl-2,3,3-trimethyl-3H-indoline-5-sulfonic acid, as pink solid.
[0048] (2) Take 2.76mmol 1-m-fluorobenzyl-2,3,3-trimethyl-3H-indoline-5-sulfonic acid, 1.38mmol 2-chloro-1-formyl-3-hydroxymethylene Cyclohexene, 2.76mmol of anhydrous sodium acetate and 20ml of absolute ethanol were added to a 100ml reaction flask, under the protection of argon, heated at reflux at 75°C for 4h, the reaction was completed, cooled to room temperature, concentrated under reduced pressure After adding concentrated methyl tert-butyl ether of 5 times the volume of the reaction solution, filter, and the filter cake is separated and purified by column chromatography (silic...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com