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A multifunctional microfluidic chip for simultaneous screening of multiple drugs and cells

A microfluidic chip, multi-functional technology, applied in the direction of drug screening, compound screening, tissue cell/virus culture device, etc., can solve the problems of inability to break through, achieve low production cost, easy integration, and reduce the possibility Effect

Active Publication Date: 2018-08-31
浙江弘瑞医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as the current technological development is concerned, the screening of multiple functions on one chip is still the bottleneck of high-throughput screening of drugs, which has become a major problem and cannot be broken through for a long time.

Method used

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  • A multifunctional microfluidic chip for simultaneous screening of multiple drugs and cells
  • A multifunctional microfluidic chip for simultaneous screening of multiple drugs and cells
  • A multifunctional microfluidic chip for simultaneous screening of multiple drugs and cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] 1. Materials and instruments.

[0069] SU-8 2075 negative photoresist; Sylgard184 polydimethylsiloxane (PDMS); curing agent (DowCorning, USA); JKG-2A photolithography machine (Shanghai Xuese Optical Machinery Co., Ltd.); HPDC- 32G-2 plasma cleaner (Harrick Plasma, USA); glue homogenizer (SC-1B, Beijing Chuangshi Weiner Technology Co., Ltd.); vacuum drying box (Shanghai Yiheng Scientific Instrument Co., Ltd.); precision syringe pump ( LSP04-1A, Baoding Lange Company); Nikon ECLIPSETI inverted fluorescence microscope (Nikon Company, Japan).

[0070] 2. Cell culture in the chip.

[0071](1) Chip pretreatment: The prepared chip is first rinsed with sterile third-grade water three times, then injected with 75% ethanol, washed three times, then washed three times with sterile water, dried, and sterilized by ultraviolet light for 30 minutes. Then inject 0.1mg·L -1 The poly-L-lysine (PLL) was incubated at 37°C for 1 hour, and the cell culture chamber 5 of the chip was coated...

Embodiment 2

[0079] 1. Materials and instruments.

[0080] SU-8 2075 negative photoresist; Sylgard184 polydimethylsiloxane (PDMS); curing agent (DowCorning, USA); JKG-2A photolithography machine (Shanghai Xueze Optical Machinery Co., Ltd.); HPDC- 32G-2 type plasma cleaning machine (Harrick Plasma Company of the United States); glue homogenizer (SC-1B type, Beijing Chuangshiweina Technology Co., Ltd.); vacuum drying oven (Shanghai Yiheng Scientific Instrument Co., Ltd.); precision syringe pump ( LSP04-1A, Baoding Lange Company); Nikon ECLIPSETI inverted fluorescence microscope (Nikon Company, Japan).

[0081] 2. Cell culture in the chip.

[0082] (1) Chip pretreatment: The prepared chip is first rinsed with sterile third-grade water three times, then injected with 75% ethanol, washed three times, then washed three times with sterile water, dried, and sterilized by ultraviolet light for 30 minutes. Then inject 0.1mg·L -1 The poly-L-lysine (PLL) was incubated at 37°C for 1 hour, and the ce...

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Abstract

The invention provides a multifunctional micro-fluidic chip used for simultaneous screening of a plurality of drugs and cells, belonging to the technical field of micro-fluidic chips. The multifunctional micro-fluidic chip used for simultaneous screening of a plurality of drugs and cells is composed of an upper PDMS micro-valve-controlled channel layer, a middle PDMS fluid channel layer and a lower glass substrate layer exerting supporting and cell attaching effect, wherein the PDMS micro-valve-controlled channel layer comprises a first micro-valve group and a second micro-valve group; the PDMS fluid channel layer comprises an array cell culture zone; and the PDMS fluid channel layer undergoes thermal bonding and is then integrated with the glass substrate layer through plasma bonding. The array cell culture zone is composed of a plurality of culture units and multiple stages of branch fluid sample introduction channels; the first micro-valve group is composed of linked micro-valves; and the second micro-valve group is composed of a plurality of independent micro-valves used for controlling connection and disconnection of a sample introduction port in a cell cavity with an S-shaped bent channel.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a multifunctional microfluidic chip for simultaneous screening of multiple drugs and cells. Background technique [0002] Drug screening is to screen out high-efficiency new drugs or lead compounds from natural or synthetic compounds, test their biological activity and pharmacological effects, and evaluate the medicinal prospects of a certain substance according to the test results. Initial process and key steps. However, the traditional drug screening process is complex, inefficient, and expensive, and cannot be adapted to the simultaneous screening of large-scale samples. Therefore, it is necessary to develop a more efficient drug screening method. In recent years, with the rapid development of cell biology technology, high-throughput and high-content screening at the cellular level has provided new information and opportunities for new drug screening. It has the a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M3/00B01L3/00
CPCB01L3/50273B01L3/502738B01L3/502753B01L2200/0668B01L2300/0883B01L2400/0475B01L2400/0487B01L2400/06C12M23/16C12N2503/02G01N33/5008G01N2500/10
Inventor 孟宪生索轶平包永睿王帅李天娇
Owner 浙江弘瑞医疗科技有限公司
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