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Burkholderia and its culture method and bacteria agent and their application

A technology of Burkholderia and Holder, applied in the application field of degrading chlorothalonil, can solve problems such as environmental pollution, high toxicity and carcinogenicity

Active Publication Date: 2019-06-25
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its content will be relatively high after repeated spraying in large quantities in the greenhouse soil, and it is difficult to degrade through the soil itself
[0003] Chlorothalonil pure product is white crystal, odorless, insoluble in water, slightly soluble in acetone and other organic solvents, low toxicity to higher animals, but more toxic to fish and crustaceans, carcinogenic to rat kidneys, Strong mutagenicity, played an important role in the prevention and control of crop diseases, insect pests and weeds, but also caused pollution to the environment
Moreover, during the production process of chlorothalonil pesticide, a large amount of pesticide-containing wastewater will be generated, and no matter how the pesticide is applied, it will inevitably enter the water body through various channels, causing water environment pollution

Method used

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  • Burkholderia and its culture method and bacteria agent and their application
  • Burkholderia and its culture method and bacteria agent and their application
  • Burkholderia and its culture method and bacteria agent and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] This embodiment is used to illustrate the morphology, physiological and biochemical characteristics of Burkholderia of the present invention

[0064] (1) Morphological characteristics

[0065] The activated bacterial strain of the present invention is serially diluted with physiological saline, and an appropriate concentration is selected to spread on the plate solid LB medium. The selection of the concentration is based on the ability to form dispersed single colonies on the LB solid medium. Then the plate was placed upside down in a 37°C incubator for cultivation, and the colony morphology was observed after a single colony was formed.

[0066] It was observed that the strain could form colonies with a diameter of 1-2 mm after being cultured on LB solid medium for 24 hours, and the colonies were milky white, round, with complete edges, convex, and opaque.

[0067] (2) Catalase test

[0068] Take the above activated culture solution, add 3% hydrogen peroxide dropwise...

Embodiment 2

[0081] This example is used to illustrate the degradation performance of Burkholderia of the present invention to chlorothalonil

[0082] (1) Degradability of chlorothalonil in water

[0083] Add chlorothalonil pesticides to the inorganic salt liquid medium in such an amount that the content of chlorothalonil is 20 mg per liter of medium, and then insert 10 volume % of the Burkhall of the present invention activated twice as above Deutschia CGMCC No.10848 was cultured at 37°C for 2 days to measure the concentration of chlorothalonil in the culture solution and calculate the degradation rate. The results are shown in Table 1.

[0084] (2) Degradability of chlorothalonil in soil phase

[0085] Add chlorothalonil to soil that is not polluted by chlorothalonil, and make the content of chlorothalonil 10 mg relative to 1 kg of soil, and then add 5ml, 10ml, 15ml and 20ml of the above-mentioned activated 2 times of the present invention respectively The Burkholderia CGMCC No.10848 c...

Embodiment 3

[0097] This embodiment is used to illustrate the remediation ability of Burkholderia of the present invention to tobacco planting soil

[0098] Spray chlorothalonil pesticides in the soil of transplanted tobacco seedlings and non-transplanted tobacco seedlings, and measure the concentration of chlorothalonil in the soil, and then spray the activated Burkholderia culture solution of the present invention in the hole , the amount of spraying is 20ml per hole. After 30 days, the concentration of chlorothalonil in the hole was measured, and the degradation rate of chlorothalonil was calculated. In each case, 3 points were selected, and then the average value was taken. The results are shown in Table 2.

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Abstract

The invention relates to the field of microorganisms, and concretely relates to a Burkholderia zhejiangensis. The preservation number of the Burkholderia zhejiangensis is CGMCC No.10848, and the Burkholderia zhejiangensis is named as P-6. The invention also discloses a culture method of the Burkholderia zhejiangensis, and an inoculant containing the Burkholderia zhejiangensis. The invention further discloses an application of the Burkholderia zhejiangensis in degradation of chlorothalonil. The Burkholderia zhejiangensis can highly-efficiently degrade chlorothalonil in water and soil, for example, the chlorothalonil degradation rate of the Burkholderia zhejiangensis in a simulated water phase in 2 d reaches up to 71.99%, and the chlorothalonil degradation rate of the Burkholderia zhejiangensis in a simulated soil phase in 2 d reaches up to 98.97%. The chlorothalonil degradation rate of the Burkholderia zhejiangensis in practical soil environment reaches up to 99.05%. The Burkholderia zhejiangensis has high economic and social benefits.

Description

technical field [0001] The present invention relates to a strain of Burkholderia, in particular to a strain of Burkholderia with the function of degrading chlorothalonil, the method for cultivating the Burkholderia contains the Burkholderia bacteria as active ingredients and their use in the degradation of chlorothalonil. Background technique [0002] Chlorothalonil (tetrachloroisophthalonitrile) is a protective stem and leaf spray fungicide substituted for benzene, which has protective and therapeutic effects. It was developed by the American Diamond Soda Company in 1963. It has various dosage forms and is widely used. It is used on various crops such as vegetables, fruit trees, beans, rice, and wheat to prevent diseases such as downy mildew, white spot, black spot, and soft rot. Chlorothalonil has good adhesion on the plant surface, is not easily washed by rain, and has stable efficacy. However, its content will be relatively high after repeated spraying in large quantit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/38
Inventor 任立伟唐丽伟林森王培红
Owner 粮华生物科技(北京)有限公司