Aliphatic amine grafted konjac glucomannan drug-loaded nano-micelle and preparation method
A technology of konjac glucomannan and glucomannan, which is applied in the direction of pharmaceutical formulations, drug combinations, antineoplastic drugs, etc., can solve the problem of less pH-sensitive micelles, and achieve reduced toxic and side effects, good biocompatibility and Biodegradability, effect of improving drug efficacy
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Embodiment 1
[0033] Example 1: Preparation of Dialdehyde Konjac Glucomannan (DAK)
[0034] Weigh 3.00 g of KGM, disperse in 500 mL of double-distilled water under mechanical stirring, and then swell at room temperature for 12 h to obtain a KGM dispersion; weigh 2.40 g of sodium periodate and dissolve in 100 mL of double-distilled water, Add it dropwise into the KGM dispersion, react for 24 h at room temperature in the dark, remove the reaction bottle, concentrate under reduced pressure at 55 °C to about 300 mL, transfer it to a dialysis bag with a molecular weight cut-off of 3500, After 7 days of dialysis, the dialyzed fluid was freeze-dried to obtain white dialdehyde konjac glucomannan (DAK).
[0035] Such as figure 1 As shown in the infrared spectrum b, at 3429 cm -1 There is a -OH stretching vibration peak at 2938 cm -1 There is a C-H stretching vibration peak at 1733 cm -1 There is a carbonyl stretching vibration peak at , indicating that C=O is produced after KGM is oxidized by s...
Embodiment 2
[0036] Embodiment 2: the preparation of octylamine grafted KGM (KGM- g -AH 8 )
[0037] Weigh 0.3 g of DAK in 30 mL of double-distilled water and dissolve at 50 °C for 10 min; weigh 0.19 mL of octylamine and dissolve it in 100 mL of ethanol, mix the ethanol solution of octylamine with DAK aqueous solution, and Reflux at the boiling point for 8 h, remove the reaction bottle, remove ethanol under reduced pressure at 30 °C, wash with ethyl acetate or chloroform three times, take the organic phase, remove the organic reagent under reduced pressure at about 30 °C, and finally dissolve it with 2 mL of ethanol, Transfer to a dialysis bag, dialyze in double distilled water for 2 days, change the water every 4 h, take out the dialyzed fluid, and freeze-dry to obtain brown grafted konjac glucomannan KGM- g -AH 8 .
[0038] Such as figure 1 As shown in the infrared spectrum of c, at 3317 cm -1 There is a -OH stretching vibration peak at 2969 cm -1 and 2924 cm -1 There is a C-H s...
Embodiment 3
[0040] Embodiment 3: the preparation of dodecylamine grafted KGM (KGM- g -AH 12 )
[0041] KGM-g-AH 12 Preparation process: Weigh 0.3 g of DAK in 30 mL of double distilled water and dissolve at 50 °C for 10 min; weigh 0.22 g of dodecylamine and dissolve it in 100 mL of ethanol, mix the ethanol solution of dodecylamine with the DAK aqueous solution , reflux at the boiling point of ethanol for 10 h, remove the reaction bottle, remove ethanol under reduced pressure at 30 °C, wash with ethyl acetate three times, take the organic phase, remove the organic reagent under reduced pressure at about 30 °C, and finally, use 2 mL of Dissolved in ethanol, transferred to a dialysis bag, dialyzed in double distilled water for 2 days, changed the water every 4 h, took out the dialyzed fluid, and freeze-dried to obtain brown grafted konjac glucomannan KGM- g -AH 12 .
[0042] Such as figure 1 As shown in the infrared spectrum of d, at 3356 cm -1 There is a -OH stretching vibration pea...
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