A kind of enzymatic synthesis method of glycosylated collagen modified polycaprolactone
A technology for degenerating collagen and polycaprolactone, which is applied in the field of enzymatic synthesis of polycaprolactone modified by glycosylated collagen, can solve the problem of collagen denaturation, loss of modification meaning, and inability to directly realize functional modification and other issues, to achieve good cell affinity, improve cell affinity, cell affinity and good adhesion
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0039] (1) Dissolve 300mg of collagen, 900mg of glucose and 1000mg of sodium cyanoborohydride in 0.2mol / L boric acid buffer (pH=9), react at 37°C for 3 days, pass through a dextran G-50 column Separation, purification, and freeze-drying to obtain glycosylated collagen;
[0040] (2) Mix ε-caprolactone monomer and glycosylated collagen evenly at a mass ratio of 1000:1, add porcine pancreatic lipase accounting for 10% of the mass of ε-caprolactone monomer, and seal with nitrogen gas It was placed in a constant temperature oscillating reactor at 30°C for 48 hours;
[0041] (3) After the reaction is completed, add dichloromethane 2 times the volume of the product to the product to completely dissolve the product, then filter out the lipase with filter paper, and then add 10 times the volume of the filtrate to the filtrate. Stand at -20°C for 24 hours and then filter to obtain the crude product;
[0042] (4) Using acetone as a solvent to extract the above crude product for 48 hours ...
Embodiment 2
[0045] (1) Dissolve 300mg of collagen, 900mg of lactose and 1000mg of sodium cyanoborohydride in 0.2mol / L boric acid buffer (pH=9), react at 37°C for 3 days, pass through a dextran G-50 column Separation, purification, and freeze-drying to obtain glycosylated collagen;
[0046] (2) Mix ε-caprolactone monomer and glycosylated collagen evenly at a mass ratio of 1:1, add Candida antarctica lipase B accounting for 0.1% of the mass of ε-caprolactone monomer, and blow nitrogen After sealing, place it in a constant temperature shaking reactor with a temperature of 100°C for 144 hours;
[0047] (3) After the reaction is completed, add dichloromethane 2 times the volume of the product to the product to completely dissolve the product, then filter out the lipase with filter paper, and then add 10 times the volume of the filtrate to the filtrate. Stand at -20°C for 24 hours and then filter to obtain the crude product;
[0048] (4) Using acetone as a solvent to extract the above crude p...
Embodiment 3
[0051] (1) Prepare glycosylated collagen according to the same method as in step (1) of Example 2;
[0052] (2) Mix ε-caprolactone monomer and glycosylated collagen evenly at a mass ratio of 400:1, add Candida antarctica lipase B accounting for 5% of the mass of ε-caprolactone monomer, and blow nitrogen After sealing, place it in a constant temperature shaking reactor at 80°C for 48 hours;
[0053] (3) After the reaction is complete, add dichloromethane to the product to dissolve the product completely, then filter out the lipase with filter paper, then add cold methanol to the filtrate, stand at -20°C and filter to obtain the crude product ;
[0054] (4) Using acetone as a solvent to extract the above crude product to obtain a glycosylated collagen-modified polycaprolactone product.
[0055] The glycosylated collagen-modified polycaprolactone was obtained by the above method, and the contact angle test was carried out after tableting, which showed that the water droplet wet...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com