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Preparation method for Chinese forest frog antioxidative peptide

An anti-oxidative peptide and snow clam technology, which is applied in anti-infective drugs, fermentation, drug combination, etc., can solve the problems of high preparation cost, cumbersome operation, unsuitable for factory production, etc., and achieve strong anti-oxidation and high enzymatic hydrolysis efficiency , The effect of shortening the preparation time

Inactive Publication Date: 2017-03-22
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is cumbersome to operate, and the preparation cost is high, so it is not suitable for factory production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method of snow clam antioxidant peptide, the specific steps are:

[0019] 1) Pretreatment: wash the clams with ice water for 3~5 times, clean the sediment and the stains on the surface of the clams, dry them in an oven at a drying temperature of 70~90°C, and a drying time of 40~60min, then crush them Crushed by machine, passed through 200-300 mesh sieve. Crushing snow clams into powder increases the specific surface area, which can make the degreasing and enzymatic hydrolysis reactions more complete, and can also shorten the reaction time;

[0020] 2) Degreasing: Add petroleum ether to the snow clam powder according to the solid-to-liquid ratio of 1:2~1:5, and the petroleum ether contains 2~4% acetone and 1~5% 2-octylphenol polyoxyethylene ether, the degreasing temperature The temperature is 60~80°C, the stirring rate is 300~400r / min, and the reaction time is 20~40min to obtain a solid-liquid separation liquid, which is suction filtered with a Buchner fun...

Embodiment 2

[0027] A preparation method of snow clam antioxidant peptide, the most preferred steps are:

[0028]1) Pretreatment: wash the clams with ice water for 4 times, clean the sediment and stains on the surface of the clams, dry them in an oven at a drying temperature of 80°C and a drying time of 50 minutes, then pulverize them with a pulverizer and pass through 250 mesh screen. Crushing snow clams into powder increases the specific surface area, which can make the degreasing and enzymatic hydrolysis reactions more complete, and can also shorten the reaction time;

[0029] 2) Degreasing: Add petroleum ether to the snow clam powder according to the solid-to-liquid ratio of 1:4, and the petroleum ether contains 3% acetone and 4% sec-octylphenol polyoxyethylene ether, the degreasing temperature is 80°C, and the stirring speed is 300r / min, the reaction time is 30min, and the solid-liquid separation liquid is obtained, which is suction-filtered with a Buchner funnel to obtain the snow ...

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Abstract

The invention provides a preparation method for Chinese forest frog antioxidative peptide. The preparation method comprises the first step of pretreatment, the second step of degreasing, the third step of enzymolysis, the fourth step of enzyme deactivation and the fifth step of antioxidative peptide preparation. The preparation method has the beneficial effects that in the enzymolysis process, an enzymolytic agent comprises components including, by weight, 5-10 parts of papain, 12-20 parts of flavourzyme, 1-3 parts of magnesium sulfate, 2-5 parts of cysteine, 1-3 parts of monocalcium phosphate, 0.5-1.5 parts of vitamin A and 2-5 parts of sodium dodecyl sulfonate. As the enzymolytic agent is used for carrying out enzymolysis on Chinese forest frog protein, conditions are mild, and the polypeptide activity cannot be destroyed; and the enzymolysis efficiency is high, and the preparation time is shortened. The Chinese forest frog antioxidative peptide prepared through the method is high in extraction rate, purity, polypeptide and antioxidative performance and suitable for industrialized production, and preparation operation is easy.

Description

technical field [0001] The invention relates to the technical field of preparation of anti-oxidation titanium, in particular to a method for preparing anti-oxidation peptide of snow clam. Background technique [0002] A large number of studies in recent years have shown that the peptides isolated from various marine biological protein hydrolysates have potential antioxidant capabilities. At present, it has been reported at home and abroad that antioxidant active peptides have been isolated from marine animals such as squid, oysters, blue mussels, cod, horse mackerel and cod. These marine biological antioxidant active peptides can effectively remove free radicals and reactive oxygen species in the body or prevent body damage caused by oxidation by blocking the free radical chain reaction in the process of lipid peroxidation. [0003] Currently, there are three main extraction methods for marine bioactive peptides: solvent extraction, microbial fermentation, and enzymatic hyd...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/20A61P31/00
CPCC12P21/06C07K1/20C07K1/34C07K1/36
Inventor 余方苗李维黄芳芳唐云平杨最素丁国芳
Owner ZHEJIANG OCEAN UNIV
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