Unlock instant, AI-driven research and patent intelligence for your innovation.

High-resistant saccharomyces cerevisiae strain for meat duck feeding and application thereof

A yeast strain and resistance technology, applied in the field of microorganisms, can solve the problems of poor resistance, starvation and high temperature resistance of yeast

Active Publication Date: 2017-03-29
WUHAN POLYTECHNIC UNIVERSITY
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, yeast has poor stress resistance, such as high temperature resistance, strong acid damage to it, and starvation resistance, which hinder its application in production practice

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-resistant saccharomyces cerevisiae strain for meat duck feeding and application thereof
  • High-resistant saccharomyces cerevisiae strain for meat duck feeding and application thereof
  • High-resistant saccharomyces cerevisiae strain for meat duck feeding and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for obtaining highly resistant duck-feeding yeast strain Saccharomyces cerevisiae WHY-7, comprising the steps of:

[0044] A. Isolation and screening of strains:

[0045] (1) Dissect and collect the contents of the digestive tract of meat ducks, dilute it 10 times with sterile saline, and culture it on malt juice agar plate at 30°C for 2 or 3 days by coating method, and pick the colonies with a certain distance from the surrounding colonies. Single colonies were further purified 2 or 3 times by plate streaking. Then pick a ring of purified bacteria and inoculate it on the slant medium of YPD (containing 10g of yeast extract powder, 20g of peptone, 20g of glucose, and 20g of agar powder per 1000ml medium), and cultivate it in a constant temperature incubator at 30°C for 2 or 3d.

[0046] (2) A single colony with a smooth surface, milky white, and neat edges grown on a solid medium plate similar to a conventional Saccharomyces cerevisiae colony was connected to...

Embodiment 2

[0069] The application of a highly resistant meat duck feeding yeast strain (microecological preparation containing yeast WHY-7) in meat duck feed comprises the following steps:

[0070] A. Select 450 1-day-old Cherry Valley meat ducks and divide them into 3 groups randomly, with 5 repetitions in each group and 30 pigeons in each repetition.

[0071] B. The test ducks are free-range in the same environment indoors. For each repetition, 1 bar, 24h light, ad libitum intake of food and water, record the amount of feed and the remaining amount of feed every day in repeat units, and clean the drinking fountains and troughs once a day.

[0072] C. Immunization, disease prevention and disinfection shall be carried out according to conventional methods. The experiment was divided into control group (basic feed), antibiotic group (basic feed+4mg flavomycin), yeast group (basal feed+1×10 9 yeast cells). The test period is 35 days. The experiment site is the farm of Wuhan Yongsheng D...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a high-resistant saccharomyces cerevisiae strain for meat duck feeding and application thereof. A preparation method of the high-resistant saccharomyces cerevisiae strain for meat duck feeding includes the steps that A, contents of meat duck digestive tracts are obtained and diluted, plate streaking is carried out, and bacterial colonies having the same morphology as conventional saccharomyces cerevisiae are selected and separated; B, the obtained saccharomyces cerevisiae is screened according to heat resistance, acid resistance, nutrient starvation endurance and bile salt tolerance; and C, the separated saccharomyces cerevisiae is subjected to physiological and biochemical identification and 26s rDNA molecular identification. The high-resistant saccharomyces cerevisiae strain for meat duck feeding belongs to saccharomyces cerevisiae and has the prominent characteristic of having the abilities of high acid resistance, heat resistance and nutrient starvation endurance. The production performance of meat ducks can be remarkably improved by adding a microecological preparation taking the high-resistant saccharomyces cerevisiae strain for meat duck feeding as a main active ingredient into compound feed for meat ducks, and the high-resistant saccharomyces cerevisiae strain for meat duck feeding can be used instead of antibiotics. The saccharomyces cerevisiae WHY-7 is preserved in China Center for Type Culture Collection, and the preservation number of the saccharomyces cerevisiae WHY-7 is CCTCC NO:M 2016550.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a yeast strain for feeding highly resistant meat ducks, and also relates to the use of a yeast strain for feeding highly resistant meat ducks. Background technique [0002] The overuse of antibiotics has posed a serious threat to the development of the poultry industry, and has caused increasing harm to the health of animals and humans. Therefore, people need to find and develop natural growth promoters that are non-toxic and have no adverse reactions, so probiotic feed additives have emerged as the times require. Probiotics can improve the production performance of animals and improve the health of animals by regulating the microecological balance of the gastrointestinal tract of animals. As a non-toxic, non-residue and non-resistant feed additive, probiotics have become one of the effective substitutes for antibiotics. [0003] Yeast (Saccharomyces cerevisiae) as a pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23K50/75A23K10/18C12R1/865
CPCC12N1/185C12R2001/865
Inventor 胡先勤王学东刘琼彭思源张霞
Owner WUHAN POLYTECHNIC UNIVERSITY