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Specific molecular marked heavy-metal chelating agent, corresponding rapid chromatographic detection card and application

A heavy metal chelating agent and molecular labeling technology, which is applied in the direction of analytical materials, measuring devices, steroids, etc., can solve the problems of failing to meet the requirements of national standards, insufficient detection sensitivity and stability, etc., and achieve easy operation, increased stability, and improved The effect of sensitivity

Inactive Publication Date: 2017-04-19
黄燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantages of this method are fast and environmentally friendly, but due to the use of competitive methods, the detection sensitivity and stability are greatly insufficient, and cannot meet the national standard requirements

Method used

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  • Specific molecular marked heavy-metal chelating agent, corresponding rapid chromatographic detection card and application
  • Specific molecular marked heavy-metal chelating agent, corresponding rapid chromatographic detection card and application
  • Specific molecular marked heavy-metal chelating agent, corresponding rapid chromatographic detection card and application

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Embodiment 1

[0030] Synthesis of the phenyl-EDTA of embodiment 1 biotin modification

[0031] (1) 55mmol phenylalanine (phenylalanine) was mixed with 25.0mL85% H 2 SO 4 dissolved and added dropwise to 12.5 mL of HNO 3 -H 2 SO 4 Mix acid Li (V / V=1.4 / 1.1), react at room temperature for 1h, then adjust the pH to 6 with ammonia water, collect the precipitate to obtain about 9g of 2-amino-3-(4-nitrophenyl)propionic acid (2- amino-3-(4-nitrophenyl) propanoic acid).

[0032] (2) The above product was dissolved in 20 mL of hydrochloric acid-saturated anhydrous methanol, hydrogen was passed for 15 minutes, refluxed at room temperature for 4 hours, and the solid was collected by freeze-drying to obtain about 8.5 g of methyl-2-amino-3(4-nitrophenyl)chloropropane Acid ester (methyl 2-amino-3-(4-nitrophenyl) propanoate hydrochloride).

[0033] (3) The above intermediate product was dissolved in 30 mL of methanol, then 5 mL of triethylamine was added, and 40 mL of saturated NH 4 OH aqueous soluti...

Embodiment 2

[0039] Synthesis of the phenyl-EDTA of embodiment 2 digoxin modification

[0040] (1) 55mmol phenylalanine with 25.0mL85% H 2 SO 4 dissolved and added dropwise to 12.5 mL of HNO 3 -H 2 SO 4 Mix acid Li (V / V=1.4 / 1.1), react at room temperature for 1h, then adjust the pH to 6 with ammonia water, collect the precipitate to obtain about 9g of 2-amino-3-(4-nitrophenyl)propionic acid (2- amino-3-(4-nitrophenyl)propanoic acid).

[0041] (2) The above product was dissolved in 20 mL of hydrochloric acid-saturated anhydrous methanol, hydrogen was passed for 15 minutes, refluxed at room temperature for 4 hours, and the solid was collected by freeze-drying to obtain about 8.5 g of methyl-2-amino-3(4-nitrophenyl)chloropropane Acid ester (methyl 2-amino-3-(4-nitrophenyl) propanoate hydrochloride).

[0042] (3) The above intermediate product was dissolved in 30 mL of methanol, then 5 mL of triethylamine was added, and 40 mL of saturated NH 4 OH aqueous solution, react at room temperat...

Embodiment 3

[0048] Synthesis of the diethylenetriaminepentaacetic acid of embodiment 3 nucleic acid modification

[0049] (1) Dissolve 35.7mg of diethylenetriaminepentaacetic acid in 1mL of DMF, add 19mg of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, react at room temperature for 30min, then add 500mg of amino DNA (NH 3 -C 3 -3'-TAGATCAGTCACGAT-5'), continue to react for 2h.

[0050] (2) After the reaction is completed, the column is purified to obtain about 500 mg of single-stranded DNA modified diethylenetriaminepentaacetic acid.

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Abstract

The invention relates to a specific molecular marked heavy-metal chelating agent, corresponding detection technologies and application. The heavy-metal chelating agent disclosed by the invention is metal chelating molecules marked by specific marking molecules; after being combined with heavy metal, one ends of the molecules can be identified and captured by an antibody; the marking molecules at the other ends are combined with a corresponding ligand or antibody; therefore, sandwich detection is realized; and the detection technologies comprise an enzyme-linked immune technology and a rapid paper chromatographic technology. By means of the specific molecular marked heavy-metal chelating agent, the corresponding detection technologies and application disclosed by the invention, rapid sandwich detection on metal ion can be realized for the first time; requirements of conventional heavy-metal detection on an expensive instrument can be avoided; and thus, disadvantages of the sensitivity and the specificity of a competition-method heavy-metal rapid detection card can be made up.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and relates to a heavy metal chelating agent based on specific molecular marker modification, a corresponding rapid chromatography detection card and its application. Background technique [0002] Heavy metals generally exist in nature in natural concentrations, but with the development of the economy, domestic mining, smelting, processing and commercial manufacturing activities of heavy metals are increasing, causing many heavy metals such as lead, mercury, cadmium, cobalt, etc. to enter the atmosphere, water, soil, causing serious environmental pollution. For a long time, heavy metal pollution has been widely concerned by people. The biological toxicity of mercury, cadmium, lead, chromium, arsenic and other heavy metal pollution caused by industrial activities is very significant, causing great harm to human health and the environment. Pollution caused by heavy metals in soil or water ente...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D495/04C07J19/00C07H21/00G01N33/531
CPCC07D495/04C07H21/00C07J19/005G01N33/531
Inventor 黄燕
Owner 黄燕