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Preparation method of chitosan-coated heat-reducing nano-silver multilayer film long-acting antibacterial coating

A technology of antibacterial coating and chitosan, which is applied in the field of preparation of long-acting antibacterial coating of heat-reduced nano-silver multilayer film, can solve problems such as reinfection, achieve simple preparation, reduce cytotoxicity, and broad-spectrum multi-functional antibacterial Effect

Active Publication Date: 2017-05-10
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the bactericidal type can effectively kill bacteria, it also faces the possibility of re-infection caused by residual bacterial "cadavers". Therefore, it is very important to develop a surface modification method that can not only effectively sterilize bacteria but also release bacterial "cadavers" in time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Prepare 0.5mg / mL PAA solution, and measure the pH value to 4.35. Take 50ml ultrapure water in a 50ml centrifuge tube, adjust the pH to 4.38, and label it as lotion 1.

[0023] Prepare 0.1mg / mL silver nitrate and dissolve it in 50ml of 1 mg / ml CHI with magnetic stirring. The pH is determined to be 3.97. Take 50ml ultrapure water and adjust the pH to 4.04 in a 50ml centrifuge tube, label it as lotion 2.

[0024] Put the pretreated substrate into PAA solution and soak for 6-8min, take it out, put it in lotion 1, wash it for 2-3min, take it out, use N 2 Blow dry. Put it into CHI-Ag + Soak in the solution for 6-8min, take it out, put it in lotion 2, wash it for 2-3min, take it out, use N 2 After drying, the preparation of a double layer is completed, and 10 double layer multilayer film coatings are prepared.

[0025] After the prepared coating was heated at 120°C for 0.5h, the size was cut to 2x8cm and 1ml of 5mg / ml CHI aqueous solution was evenly smeared on the surface and dried...

Embodiment 2

[0028] Prepare a dopa solution of 1 mg / mL, and measure the pH to be 4.36. Take 50ml ultrapure water in a 50ml centrifuge tube, adjust the pH to 4.34, and label it as lotion 1.

[0029] Prepare 0.5mg / mL silver nitrate and dissolve it in 50ml of 2 mg / ml CHI with magnetic stirring. The pH is determined to be 4.0. Take 50ml ultrapure water and adjust the pH to 4.20 in a 50ml centrifuge tube, label it as lotion 2.

[0030] Put the pretreated substrate into PAA solution and soak for 6-8min, take it out, put it in lotion 1, wash it for 2-3min, take it out, use N 2 Blow dry. Put it into CHI-Ag + Soak in the solution for 6-8min, take it out, put it in lotion 2, wash it for 2-3min, take it out, use N 2 Blow dry. So far, the preparation of a double layer is completed. Prepare 3, 6, 9, 12, 15 double layers respectively.

[0031] After heating the prepared coating at 130°C for 1 hour, cut the size to 2x8cm and evenly smear 2ml of 5mg / ml CHI aqueous solution on the surface, and dry it in an ov...

Embodiment 3

[0034] A 2mg / mL dopa solution was prepared, and the pH was determined to be 3.96. Take 50ml ultrapure water in a 50ml centrifuge tube, adjust the pH to 4.0, and label it as lotion 1.

[0035] Prepare 1mg / mL silver nitrate and dissolve it in 50ml 2.5mg / ml CHI with magnetic stirring, and measure the pH to be 4.96. Take 50ml ultrapure water and adjust the pH to 4.93 in a 50ml centrifuge tube, label it as lotion 2.

[0036] Put the pretreated substrate into PAA solution and soak for 6-8min, take it out, put it in lotion 1, wash it for 2-3min, take it out, use N 2 Blow dry. Put it into CHI-Ag + Soak in the solution for 6-8min, take it out, put it in lotion 2, wash it for 2-3min, take it out, use N 2 Blow dry. So far, the preparation of a double layer is completed. Prepare 3, 6, 9, 12 double layers respectively.

[0037] After heating the prepared coating at 140°C for 1 hour, cut the size to 2x8cm and evenly smear 3ml of 5mg / ml CHI aqueous solution on the surface, and dry it in an oven...

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Abstract

The invention relates to a preparation method of a chitosan-coated heat-reducing nano-silver multilayer film long-acting antibacterial coating. The coating is prepared by using a solution of polyacrylic acid and chitosan-silver nitrate and a layer-by-layer self-assembly method; the heat-reducing nano-silver organic-inorganic hybrid hydrogel coating is obtained by using a heating way. By coating the surface of a multilayer film with a layer of chitosan, the release rate of nano-silver is controlled, and the biocompatibility of the multilayer film is improved. Silver ions have a broad-spectrum antibacterial function, so that the slow release of the silver ions enables the multilayer film to have an efficient and long-acting antibacterial ability for gram-positive and gram-negative bacteria; By coating the surface with the layer of chitosan, the cytotoxicity for fibroblasts and human lens epithelial cells can be reduced, and the biocompatibility is improved. The method is simple and fast in technology and easy to smear, dip and spray, can be implemented in an industrial way, and has wide application scope; the chitosan-coated heat-reducing nano-silver multilayer film long-acting antibacterial coating can effectively improve the antibacterial property and biocompatibility of the surface of a medical device.

Description

Technical field [0001] The invention specifically relates to the technical field of new materials, in particular to a preparation method of a chitosan-coated thermal reduction nano silver multilayer film long-lasting antibacterial coating. Background technique [0002] The application of medical biomaterials is becoming more and more extensive. While improving the medical level, we also have to face a series of problems that it brings. Among them, the most urgent need to solve is the problem of biomaterial infection. The process of infection of biological materials can be simply described as: microorganisms adsorb on the surface of biological materials, then colonize to form colonies, and then form a dense biofilm, release planktonic bacteria and toxins, and finally cause infection. More than 95% of bacteria in nature can form biofilms through the above methods. In the United States, more than 100,000 people die from infections related to biological materials each year. Therefo...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L31/16
Inventor 王佰亮南开辉徐青文陈浩刘慧华叶子
Owner WENZHOU MEDICAL UNIV
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