Platinum-sulfonated graphene composite nano material with characteristics of simulated peroxidase

A technology of composite nanomaterials and sulfonated graphene, which is applied in the field of nanotechnology and bionics, can solve the problems of reducing the catalytic activity of platinum nanoparticles, complex operation of the synthesis process, and long preparation time, and achieve good peroxidase activity and sensitivity High, low detection limit effect

Inactive Publication Date: 2017-05-10
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The composite material prepared with the above-mentioned stabilizer overcomes some defects of platinum nanoparticles to a certain extent, but its synthesis process is complicated, consumes a lot of reagents and takes a long time to prepare, or reduces the catalytic activity of the original platinum nanoparticles.

Method used

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  • Platinum-sulfonated graphene composite nano material with characteristics of simulated peroxidase
  • Platinum-sulfonated graphene composite nano material with characteristics of simulated peroxidase
  • Platinum-sulfonated graphene composite nano material with characteristics of simulated peroxidase

Examples

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example 1

[0041] Graphene oxide was prepared by the Hummers method: 2.7 g of 325 mesh flake graphite was weighed and added to a mixed solution of 316 mL of concentrated sulfuric acid with a concentration of 18.4 mol / L and 36 mL of phosphoric acid with a concentration of 14.7 mol / L. After stirring thoroughly, place in an ice bath at 0 °C. Slowly add 16.2 g of potassium permanganate in small amounts to the above mixed solution. Keep the ice bath at 0 °C, stir with magnetic force for 3 hours, transfer the obtained dark green suspension to a warm water bath at 35 °C, control the temperature for 1 hour, then transfer the obtained dark green suspension to a warm water bath at 50 °C, and control the temperature for 12 hours A purple-black suspension mixture was obtained. 360 mL of ice water was slowly added to the obtained purple-black suspension mixture, and stirred vigorously for 1 hour. Then, 12 mL of 30wt% hydrogen peroxide solution was added dropwise to the solution, the color of the so...

example 2

[0043] Preparation of diazonium salt of p-aminobenzenesulfonic acid: 10 mL of 5 wt% sodium carbonate solution and 2.1 g of p-aminobenzenesulfonic acid crystals were placed in a 150 mL four-necked bottle equipped with a condenser, stirrer, dropping funnel and thermometer , warm to dissolve under stirring, then add a solution made of 0.8 g sodium nitrite and 6 mL water, and cool to 0~5 °C with an ice bath. Under continuous stirring, a solution of 3 mL of concentrated hydrochloric acid and 10 mL of water was added dropwise into the above mixed solution, and stirring was continued for 15 min in an ice bath to obtain the diazonium salt of p-aminobenzenesulfonic acid.

example 3

[0045] Graphene oxide prepared by the Hummers method was dispersed in deionized water, and ultrasonically treated for 3 hours to obtain a dispersion system of 4 mg / mL graphene oxide. Add 5wt% sodium carbonate solution to the graphene oxide dispersion system, so that the pH of the system is 9~10, then add 30 mL of sodium borohydride solution with a concentration of 0.16 g / mL, stir and react at 80 °C for 1 hour, 8000 rpm After centrifugation, the precipitate was washed with water to obtain neutral reduced graphene oxide. Disperse reduced graphene oxide in deionized water to obtain 75 mL of reduced graphene oxide suspension, then add diazonium p-sulfanilate to the reduced graphene oxide suspension and stir for 2 hours in an ice bath , after centrifugation, the precipitate was washed with double distilled water until the pH value was 7, and a neutral conjugate was obtained. Disperse the coupling compound obtained above in 150 mL of deionized water, add 10 mL of hydrazine hydrate ...

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Abstract

The invention discloses a platinum-sulfonated graphene composite nano material with characteristics of simulated peroxidase. Sulfonated graphene is taken as the substrate, platinum nano particles are synthesized by a sodium borohydride reduction method, and sulfonated graphene and platinum nano particles are used to prepare the platinum-sulfonated graphene composite nano material. The platinum-sulfonated graphene composite nano material has an excellent activity of peroxidase and can catalyze the oxidation reactions between 3,3',5,5'-tetramethylbenzidine hydrochloride to trigger color development. The composite nano material has good affinity on the substrate namely 3,3',5,5'-tetramethylbenzidine hydrochloride. The composite nano material can be used to detect hydrogen peroxide with a low concentration. The absorbance of catalytic product and the concentration (0.01-0.1 mmol/L) of hydrogen peroxide are in a linear relationship, and the detection limit is 0.0025 mmol/L.

Description

technical field [0001] The invention relates to a platinum-sulfonated graphene composite nanomaterial with the characteristic of simulating peroxidase, and belongs to the field of nanotechnology and bionic technology. Background technique [0002] Natural enzymes are mainly proteins, and a small amount are nucleic acids, which play an important role in maintaining the basic processes of biological life activities and chemical reactions. Due to the specificity of enzymes for substrate binding and their high catalytic activity, enzymes have attracted much attention in various fields. However, many disadvantages of natural enzymes, such as limited sources, complex and difficult purification, harsh storage conditions, and vulnerability to denaturation and inactivation caused by various physical and chemical environments during operation, limit the application of natural enzymes. Artificial mimetic enzymes with similar functions to natural enzymes can overcome the above-mentione...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J27/02B01J23/42B01J21/18G01N21/78
CPCB01J27/02B01J21/18B01J23/42G01N21/78
Inventor 陈伟王艳红邓豪华彭花萍黄种南
Owner FUJIAN MEDICAL UNIV
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