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Preparation of N,S codoped graphene quantum dot having pH sensitive response and determination of urease activity

A graphene quantum dot, co-doping technology, applied in measurement devices, color/spectral property measurement, nanotechnology for materials and surface science, etc., can solve the problems of enzyme activity destruction, cumbersome process, reduction, etc. Easy operation, high sensitivity, high quality effect

Inactive Publication Date: 2017-05-10
FUJIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Then, many literatures report the qualitative detection of urease, or the activity of the enzyme is destroyed or reduced during the determination process, and the process is also relatively cumbersome.

Method used

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  • Preparation of N,S codoped graphene quantum dot having pH sensitive response and determination of urease activity
  • Preparation of N,S codoped graphene quantum dot having pH sensitive response and determination of urease activity
  • Preparation of N,S codoped graphene quantum dot having pH sensitive response and determination of urease activity

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Embodiment 1

[0043] (2) Example 1 (Synthesis of N,S-GQDs)

[0044] The N, S co-doped graphene quantum dots of the present embodiment are prepared through the following steps:

[0045] Accurately weigh 2.0 g of citric acid and 0.6 g of glutathione, place them in a 50ml round bottom flask, and react in an oil bath at 150°C for 20 minutes. After 20 minutes, citric acid and glutathione have been completely liquefied, and the color is as follows: Colorless to yellowish brown, then add 10ml of polyethylene polyamine, adjust the temperature to 200°C, and continue to stir for 1h. After the reaction finishes, take out the brownish-yellow viscous liquid obtained, after natural cooling, add 50ml acetone solution, after the white solid is separated out, centrifuge to obtain pale yellow N, S-GQDs powder, use the graphene quantum dot that centrifugation obtains 0.5mL of distilled water was dissolved, purified by dialysis and freeze-dried to obtain graphene quantum dot solids. Among them, the product d...

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Abstract

The invention discloses preparation of N,S codoped graphene quantum dot having pH sensitive response and determination of urease activity. A preparation method comprises the steps that a carbon source compound and a sulfur source compound perform hydrothermal reaction, then a nitrogen source compound is added to obtain graphene quantum dot having pH response. The steps of the preparation method are simple, the used source materials are common, the cost is low, the yield is high, and the impurity amount is small. Therefore, popularization and application of the graphene quantum dot are promoted. The N,S codoped graphene quantum dot is stable in structure and rich in group on surface, has good fluorescence property and has good linearity in the pH range of 3-9. Due to the fact that OH- and NH<4+> are generated due to urea hydrolysis of urease, a pH value of a whole solution system is improved, the changing fluorescence intensity is further determined through the N,S codoped graphene quantum dot having the pH sensitive response and used for detecting the urease activity, and the lowest detection limit of the urease activity can be up to 2.5 U / L.

Description

technical field [0001] The invention relates to the field of preparation of carbon nanomaterials, in particular to a preparation method of N and S co-doped graphene quantum dots and its application in the detection of urease activity. Background technique [0002] (1) Graphene Quantum Dots (Graphene Quantum Dots, GQDs), as a new type of zero-dimensional carbon nanomaterials, due to quantum confinement and size, edge effects, graphene quantum dots have many unique optical properties: strong light Luminescent properties and the controllability of its color according to the size and elemental composition of quantum dots; it also has extremely narrow and symmetrical PL emission. At the same time, it has good biocompatibility, low cytotoxicity, environmental friendliness and low preparation cost. Due to the optical properties of GQDs, it can be applied to the detection of small molecular substances and negative (positive) ions in the body, biological imaging, drug delivery, and ...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y40/00B82Y30/00B82Y20/00G01N21/64G01N21/33
CPCC09K11/65B82Y20/00B82Y30/00B82Y40/00G01N21/33G01N21/6428
Inventor 翁少煌林新华黄丽英潘清清郭茹彬
Owner FUJIAN MEDICAL UNIV
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