A kind of alkalophilic bacillus ntt33c6d2 and its application
A technology of alkalophilic bacillus and strains, applied in the field of microorganisms, can solve problems such as low enzyme activity, achieve high production stability, avoid secondary pollution, and reduce production costs
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Embodiment 1
[0043] A method for isolation and screening of alkalophilic bacillus NTT33C6D2, the steps of which are:
[0044] 1) Sample collection
[0045] Soil sampling was carried out from the Zhelimu League area of Inner Mongolia Autonomous Region, China. Suspend 1g of the soil sample in sterile water, mix well, and put the supernatant into the glucose medium. The ingredients are: glucose 10g; peptone 5g; yeast extract 5g; K 2 HPO 4 1g; MgSO 4 ·7H 2 O 0.2g; Agar 18g; Water 1000mL; 2 CO 3 Adjust the pH to 10-11; incubate at 28°C for 24-48 hours.
[0046] 2) Separation and purification
[0047] Select a single colony in the above-mentioned medium and transfer it to the solid medium of locust bean gum, and its composition is: locust bean gum 10g, yeast extract 5g, peptone 5g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.1g, agar 18g, water 1000mL, with Na 2 CO 3 Adjust the pH to 10-11; incubate at 28°C for 24-36 hours, select the strains that produce a transparent circle around the colon...
Embodiment 2
[0069] A kind of application of alkalophilic bacillus NTT33C6D2 in ramie production, its steps are:
[0070] 1) Put the mutant strain NTT33C6D2 in locust bean gum liquid medium and activate it by culturing it at 37°C for 20-24 hours; then transfer it to the medium containing ramie, culture it with shaking at 37°C and 180rpm, and take samples at different times (every 4 hours) Observe its fiber dispersion situation, and measure residual glue rate (table 1), measure analysis method such as People's Republic of China " ramie chemical composition quantitative analysis method " (GB5889-86); Contain the substratum composition of ramie to be ramie 10g, (NH 4 ) 2 SO 4 1.5g, K 2 HPO 4 0.2g, MgSO 4 ·7H 2 O 0.25g, water 150mL, with Na 2 CO 3 Adjust the pH to 10-11, inoculate after sterilization.
[0071] Table 1 Degumming of ramie biodegumming residual gum rate
[0072]
[0073] 2) The mutant strain is used for biological degumming of ramie with burlap removed, and the degu...
Embodiment 3
[0075] A kind of application and effect evaluation of alkalophilic bacillus NTT33C6D2 in ramie production, its steps are:
[0076] 1) carry out biological degumming 6-8 hour to ramie according to the method described in step 1) in embodiment 2;
[0077] 2) After degumming, the ramie dispersed like cotton is washed once with water, and then boiled for 1-2 hours in a solution containing 0.3-0.5% NaOH and 0.1% mass concentration of sodium tripolyphosphate solution at 0.2Mpa;
[0078] 3) According to the general treatment method of chemical degumming (Ouyang Shu, Yao Gang, review and application of new technology of chemical degumming of ramie [J]. Ramie Textile Science and Technology, 1993,16(1):30-32.) Washing and beating , bleaching (available chlorine 0.5-0.8g / L), washing, pickling (pH2.5), washing, drying, after oiling, drying, drying, to obtain dry hemp. The quality standard shown, measuring analysis method such as People's Republic of China "ramie chemical composition quan...
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