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Beta-galactosidase fluorescent sensor based on silicon nanowire as well as preparation method and application of beta-galactosidase fluorescent sensor

The technology of galactosidase and fluorescence sensor is applied in the field of nanostructured fluorescence chemical sensor, which can solve the problems of time-consuming and low covalent connection efficiency, and achieve the effects of saving time, less damage and simple preparation method.

Active Publication Date: 2017-05-17
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, these silicon nanowire fluorescence sensors are all based on the modification of the oxide layer on the surface of silicon nanowires. Covalently modifying molecules to the surface of silicon nanowires is time-consuming and the efficiency of covalent connection is low.

Method used

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  • Beta-galactosidase fluorescent sensor based on silicon nanowire as well as preparation method and application of beta-galactosidase fluorescent sensor
  • Beta-galactosidase fluorescent sensor based on silicon nanowire as well as preparation method and application of beta-galactosidase fluorescent sensor
  • Beta-galactosidase fluorescent sensor based on silicon nanowire as well as preparation method and application of beta-galactosidase fluorescent sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation method of a fluorescent sensor for detecting β-galactosidase based on a single silicon nanowire

[0042] A method for preparing a fluorescent sensor for detecting β-galactosidase based on a single silicon nanowire, comprising the following steps:

[0043] 1) At room temperature, silver oxide and p-hydroxybenzaldehyde were added to the acetonitrile solution of 2,3,4,6-tetraacetyl-α-D-pyranose bromide and stirred for 14 hours, filtered and purified to obtain product 1; Wherein, the molar ratio of silver oxide, p-hydroxybenzoic acid, and 2,3,4,6-tetraacetyl-α-D-pyranose bromide is 2:1:1.

[0044] 2) Dissolve the product 1 in step 1) in a mixed solution of chloroform and isopropanol (v / v=25 / 8), put it in an ice bath, add sodium borohydride in batches=stir at room temperature for 3 hours, Spot the plate to track the reaction, add 10% sodium citrate solution to quench the reaction, wash three times with 10% sodium bicarbonate solution, wash once with wa...

Embodiment 2

[0051] Example 2 Preparation method of a fluorescent sensor for detecting β-galactosidase based on a single silicon nanowire

[0052] A method for preparing a fluorescent sensor for detecting β-galactosidase based on a single silicon nanowire, comprising the following steps:

[0053] 1) At room temperature, silver oxide and p-hydroxybenzaldehyde were added to the acetonitrile solution of 2,3,4,6-tetraacetyl-α-D-pyranose bromide and stirred for 16 hours, filtered and purified to obtain product 1; Wherein, the molar ratio of silver oxide, p-hydroxybenzoic acid, and 2,3,4,6-tetraacetyl-α-D-pyranose bromide is 1:1:1.

[0054] 2) Dissolve the product 1 in step 1) in a mixed solution of chloroform and isopropanol (v / v=25 / 8), put it in an ice bath, add solid sodium borohydride in batches and stir at room temperature for 4 hours, Spot the plate to track the reaction, add 10% sodium citrate solution to quench the reaction, wash with 10% sodium bicarbonate solution three times, wash on...

Embodiment 3

[0061] Example 3 Preparation method of a fluorescent sensor for detecting β-galactosidase based on a single silicon nanowire

[0062] A method for preparing a fluorescent sensor for detecting β-galactosidase based on a single silicon nanowire, comprising the following steps:

[0063] 1) At room temperature, silver oxide and p-hydroxybenzaldehyde were added to the acetonitrile solution of 2,3,4,6-tetraacetyl-α-D-pyranose bromide and stirred for 18 hours, filtered and purified to obtain product 1; Wherein, the molar ratio of silver oxide, p-hydroxybenzoic acid, and 2,3,4,6-tetraacetyl-α-D-pyranose bromide is 2:1:2.

[0064] 2) Dissolve the product 1 in step 1 in a mixed solution of methyl chloride and isopropanol (v / v=25 / 8), put it in an ice bath, add sodium borohydride in batches and stir at room temperature for 5 hours, spot the plate Follow up the reaction, add 10% sodium citrate solution to quench the reaction, wash three times with 10% sodium bicarbonate solution, wash onc...

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Abstract

The invention discloses a fluorescent sensor for detecting beta-galactosidase based on a single silicon nanowire. The fluorescent sensor is the single silicon nanowire with a surface physically adsorbing beta-galactosidase specific response molecules, wherein the beta-galactosidase specific response molecules are formed by carrying out esterification on pyranose molecules capable of responding with the beta-galactosidase, and connecting fluorescent molecules 4-amino-1,8-naphthalic anhydride. The invention further discloses a preparation method and application of the fluorescent sensor. The preparation method of the fluorescent sensor is simple, the time is shortened, and the efficiency of the sensor is not influenced; the fluorescent sensor also has a double-input AND logic gate functions of esterase and the beta-galactosidase, and a new method is provided for directly detecting the beta-galactosidase in cells; the fluorescent sensor has a wide application prospect in the aspect of detecting the beta-galactosidase on a single cell level.

Description

technical field [0001] The invention relates to the field of fluorescent chemical sensors with nanostructures. More specifically, it relates to a silicon nanowire-based β-galactosidase fluorescent sensor and its preparation method and application. Background technique [0002] β-galactosidase as a biological beacon molecule is often used in the detection of cell metabolism and targeted determination of the distribution of cancer cells. The general probe molecular detection of β-galactosidase is a single-wavelength detection, which is greatly affected by the background fluorescence, and due to the hydrophobic effect of the probe molecule, its stability in the cell is poor, and it is rapidly degraded with cell metabolism. Expelled out of the cell, resulting in poor accuracy of the test results. Immobilizing probe molecules on nanocarriers can not only realize small-scale detection but also prevent probe molecules from leaving with cell metabolism. [0003] Silicon nanomater...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/06
CPCC09K11/06C09K2211/1088G01N21/6428
Inventor 穆丽璇陈敏师文生
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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