A kind of bacterial strain used for producing succinic acid by fermentation of biodiesel by-product crude glycerol and production method thereof

A technology for producing succinic acid and biodiesel, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of high cost of raw materials, and achieve the effect of simple production method, reduction of production cost, and extension of industrial chain.

Active Publication Date: 2021-03-30
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to solve the problem of high cost of raw materials in the current production of succinic acid fermentation, to provide a bacterial strain and production method for producing succinic acid by fermenting crude glycerol, a by-product of biodiesel

Method used

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  • A kind of bacterial strain used for producing succinic acid by fermentation of biodiesel by-product crude glycerol and production method thereof
  • A kind of bacterial strain used for producing succinic acid by fermentation of biodiesel by-product crude glycerol and production method thereof
  • A kind of bacterial strain used for producing succinic acid by fermentation of biodiesel by-product crude glycerol and production method thereof

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Experimental program
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Effect test

Embodiment 1

[0029] Breeding and identification of Actinobacillus succinate

[0030] 1. The method of directed evolution breeding of Actinobacillus succinogenes is as follows: first use 10g / L glycerol to replace the glucose in the seed solution as a carbon source as a directed medium, place it in an anaerobic incubator after inoculation, and keep it at 37°C Cultivate for 12-18 hours for subculture. After 7-10 days of cultivation, the concentration of glycerol increases to 20g / L, and so on. After about 90 days of domestication, the concentration of glycerol in the medium can be increased to 80g / L. XT, the strain can grow well in the culture set with a glycerol concentration of 80g / L.

[0031] 2. The 16S rDNA gene identification of the Actinobacillus succinogenes XT obtained from the above selection, and the physiological and biochemical identification according to the "Berger's Bacteria Identification Manual", the results are as follows:

[0032] Actinobacillus succinogenes XT is 100% simila...

Embodiment 2

[0035] Using Actinobacillus succinogenes CCTCC NO: M2016397 to ferment biodiesel by-product crude glycerol to produce succinic acid, the steps are as follows:

[0036] (1) Preparation of seed medium: Mix the ingredients with the following concentrations and sterilize at 115°C for 20 minutes to obtain the seed medium: glucose 20g / L, yeast powder 10g / L, corn steep liquor 4g / L, NaHCO 3 4g / L, NaH 2 PO 4 9.6g / L, K 2 HPO 4 1.55g / L.

[0037] (2) Preparation of fermentation medium: Mix the ingredients with the following concentrations and sterilize at 115°C for 20 minutes to obtain a fermentation medium: crude glycerol 30g / L, nitrogen source 5g / L, potassium dihydrogen phosphate 2g / L, dihydrogen phosphate Methyl sulfoxide 0g / L, sodium bicarbonate 2g / L, calcium chloride 0.3g / L, magnesium chloride 0.3g / L.

[0038] The above-mentioned nitrogen source is corn steep liquor, yeast powder, soybean powder, and peanut cake powder mixed in any proportion.

[0039] The above-mentioned pH ...

Embodiment 3

[0049] Using Actinobacillus succinogenes CCTCC NO: M2016397 to ferment biodiesel by-product crude glycerol to produce succinic acid, the steps are as follows:

[0050] (1) Preparation of seed medium: Mix the ingredients with the following concentrations and sterilize at 115°C for 20 minutes to obtain the seed medium: glucose 20g / L, yeast powder 10g / L, corn steep liquor 4g / L, NaHCO 3 4g / L, NaH 2 PO 4 9.6g / L, K 2 HPO 4 1.55g / L.

[0051] (2) Preparation of fermentation medium: Mix the ingredients with the following concentrations and sterilize at 115°C for 20 minutes to obtain a fermentation medium: crude glycerol 100g / L, nitrogen source 20g / L, potassium dihydrogen phosphate 10g / L, dihydrogen phosphate Methyl sulfoxide 20g / L, sodium bicarbonate 10g / L, calcium chloride 1.0g / L, magnesium chloride 1.0g / L.

[0052] The nitrogen source mentioned above is corn steep liquor and yeast powder mixed in any proportion.

[0053] The above-mentioned pH regulator is basic magnesium car...

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Abstract

The invention discloses a bacterial strain used to produce succinic acid by fermenting crude glycerol, a by-product of biodiesel, and a production method thereof. The bacterial strain Actinobacillus succinoproducens ( Actinobacillus succinogenes ) XT, deposit number CCTCC NO: M2016397, can use biodiesel by-product crude glycerol as raw material to ferment and produce succinic acid, the yield of succinic acid can reach up to 72 g / L, and the yield can reach up to 95%. The production method of the present invention is simple, efficient, and low in cost, realizes the efficient resource utilization of industrial waste crude glycerin, solves the environmental problems caused by the discharge of biodiesel by-product glycerin, extends the biodiesel industry chain, and improves the biodiesel industry. Overall technical level and circular benefits.

Description

technical field [0001] The invention belongs to the technical field of industrial microorganism fermentation, and in particular relates to a strain used for producing succinic acid by fermenting crude glycerol, a by-product of biodiesel, and a production method thereof. Background technique [0002] Succinic acid, also known as succinic acid, is an important C4 platform compound. The basic raw material of chemicals and special chemicals, widely used in food, plastics, medicine, spices and other industries, can replace benzene to synthesize more than 250 kinds of chemical products. Among them, the most promising field is synthetic plastics, which are synthetic polybutylene succinate (PBS), polyethylene glycol succinate (PES), polypropylene glycol succinate (PPS), etc. The main raw material of degradable polymer materials. In 2004, among the 12 most potential bulk products announced by the US Department of Energy, succinic acid ranked first. According to the forecast of Tra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/46C12R1/01
CPCC12P7/46C12N1/205C12R2001/01
Inventor 秦艳申乃坤王青艳朱婧李亿
Owner GUANGXI ACAD OF SCI
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