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Preparation process of selenium-enriched protein peptide

A technology of selenoenriched protein and preparation process, which is applied in the field of preparation technology of selenoenriched protein peptide, and achieves the effects of easy digestion and absorption, improvement of body immunity and high added value

Inactive Publication Date: 2017-05-31
CHONGQING DOUHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the application of porcine blood protein peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation process of seleno-enriched protein peptide, the steps are as follows: (1) Preparation of protein peptide enzymatic hydrolysis solution: a) Collect fresh pig blood, anticoagulate with 5g / L sodium citrate, and centrifuge at 4°C and 8000r / min After 10 minutes, collect the lower layer of sediment to obtain red blood cells; add the red blood cells to the same volume of normal saline to wash and centrifuge twice, then add 2 times the volume of distilled water, ultrasonic cell disruption assists hemolysis for 20 minutes, and then centrifuge at 4°C and 4000r / min for 10 minutes to obtain Store the hemoglobin solution at 4°C for later use; b) Take the hemoglobin solution, add an appropriate amount of trypsin to enzymolyze it, stir, keep the system pH 8.0 with 2mol / L NaoH solution, keep it at 45-50°C for 6-10 hours; Raise the temperature to 85°C and maintain this temperature for 10-15 minutes, then quickly cool down to below 50°C, adjust the pH value to about 7.0, cen...

Embodiment 2

[0022] A preparation process of seleno-enriched protein peptide, the steps are as follows: (1) The preparation of protein peptide enzymatic hydrolysis solution is the same as that in Example 1; (2) Carboxymethyl chitosan, sodium selenite and citric acid are prepared in a mass ratio of Mix 100:1:15, prepare nano-selenium solution in the water phase, control the temperature at 60°C, and the mass fraction of chitosan in the solution is 5×10 -4 , the concentration of the selenium compound is 3mmol / L, and the concentration of citric acid is 7g / L; (3) adding the nano-selenium solution into the enzymolysis solution with a temperature of 55°C for chelation for 2h to obtain a solution; (4) adding the The finished product is obtained by concentrating the liquid and spray drying, wherein the air inlet temperature is 220°C and the air outlet temperature is 90°C during spray drying.

Embodiment 3

[0024] A preparation process of seleno-enriched protein peptide, the steps are as follows: (1) The preparation of protein peptide enzymatic hydrolysis solution is the same as that in Example 1; (2) Carboxymethyl chitosan, sodium selenite and vitamin C are prepared according to the mass ratio of Mix 90:1:12, prepare nano-selenium solution in the water phase, keep the temperature at 65°C for 2 hours, then ultrasonic treatment at 100W, 15kHz for 30 minutes, treatment for 6 minutes with an interval of 7 minutes, the mass fraction of chitosan in the solution is 5 ×10-4, the concentration of selenium compound is 3mmol / L, and the concentration of citric acid is 6g / L; (3) Add the nano-selenium solution into the enzymolysis solution at a temperature of 60°C, and chelate for 1.5h to obtain a liquid; (4) Concentrate the liquid and spray dry to obtain the finished product, wherein the air inlet temperature is 230°C and the air outlet temperature is 100°C during spray drying.

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Abstract

The invention relates to a preparation process of a selenium-enriched protein peptide. The preparation method comprises the following steps: preparing protein peptide enzymatic hydrolysate: taking fresh pig blood as a raw material, and carrying out hemolysis, enzymolysis, decoloration and ultrafiltration to obtain the protein peptide enzymatic hydrolysate; secondly, mixing chitosan, a selenium compound and citric acid according to certain proportions, and preparing a nano selenium solution in an aqueous phase; thirdly, adding the nano selenium solution into the protein peptide enzymatic hydrolysate with the temperature of 55 to 65DEG C to obtain a mixed solution, heating the mixed solution to be 45 to 60DEG C and reacting for 1 to 2 hours to obtain a finished solution; fourthly, concentrating and drying the finished solution to obtain a finished product. According to the preparation method disclosed by the invention, protein resources are sufficiently utilized; moreover, pollution to environment is also avoided; the chitosan is used as a medium for adsorbing nano selenium and polypeptide to form multifunctional active peptide protein powder rich in the nano selenium, a short chain peptide and balanced amino acid; the selenium-enriched protein peptide is high in nutrient value and easy to ingest and can be used for preventing multiple diseases.

Description

technical field [0001] The invention relates to the field of dietary nutrition, in particular to a preparation process of seleno-enriched protein peptide. Background technique [0002] Pig blood protein peptide is obtained from pig blood through hemolysis, enzymatic hydrolysis, decolorization, ultrafiltration and drying. Porcine blood protein peptide is a high-quality protein raw material, which contains various functional proteins such as growth factors and small molecular peptides. It has the characteristics of good palatability, high protein content, balanced amino acids, high absorption and utilization rate, and high added value. Protein peptide has a significant effect on improving iron deficiency anemia and regulating immunity, and can be widely used in the fields of food, health food and medicine. At present, the application of porcine blood protein peptide is rarely reported. Contents of the invention [0003] The purpose of the invention is to disclose a prepara...

Claims

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Application Information

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IPC IPC(8): A23J1/06A23J3/12A23J3/34
Inventor 邓小玲邓焱飞其他发明人请求不公开姓名
Owner CHONGQING DOUHAO BIOTECH
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