Nori small peptide chelate manganese compound and preparation method as well as application thereof

A small peptide chelation and complex technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of unfavorable industrial production and use, long preparation time, etc., and achieve improved utilization value, rapid dissolution, and good stability. Effect

Inactive Publication Date: 2017-05-31
GUANGZHOU AONONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned patented technology uses microbial fermentation to extract protein and then prepares small peptides by proteolysis; or first enzymatically hydrolyzes and then microbiologically ferments to prepare protein small peptides, and then adds water-soluble manganese salts to prepare small peptide chelated manganese complexes, which takes a long time to prepare. It is not conducive to industrial production and use, and conventional protein raw materials are used, such as soybean meal, rice protein powder, wheat gluten powder, corn gluten meal, cotton meal, peanut meal, potato protein

Method used

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  • Nori small peptide chelate manganese compound and preparation method as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take 500g of seaweed as protein raw material, mash it with water, add deionized water according to the ratio of material to liquid 1:50, heat up to 40°C, natural pH, add manganese ion amount 0.1mg / mL, add cellulase and fruit Glue enzyme, the amount of enzyme added is 0.1g / 100mL, the compound ratio is 1:1, enzymolysis is 1h, and the enzyme is inactivated (temperature 100°C, time 20min); after the above enzymolysis solution is cooled to 50°C, add neutral protease and papain, The amount of enzyme added is 0.1g / 100mL, the compound ratio is 1:5, the enzymolysis is 0.5h, the enzyme is inactivated (temperature 100°C, time 30min), centrifuged (speed 6000rpm, time 15min), the chelation rate is measured, and the quantitative enzymolysis solution is taken Freeze-dry to obtain a grade I laver chelated manganese salt with a chelation rate of 20% (±5%), which is recorded as Experiment No. 1.

Embodiment 2

[0030] Take the enzymolysis solution in (1), control the temperature at 40°C, stir and shake for 0.5h, measure the chelation rate (Yang Shen, 2008), centrifuge, freeze-dry, and obtain II with a chelation rate of 40% (± 5%) Grade laver small peptide chelated manganese salt, recorded as Experiment No. 2.

Embodiment 3

[0032] Take 500g of seaweed as the protein material, mash it with water, add deionized water according to the material-to-liquid ratio of 1:30, heat up to 50°C, and add manganese ions of 0.08mg / mL at the natural pH, and add cellulase and fruit at the same time Glue enzyme, add enzyme amount 0.5g / 100mL, compound ratio 1:2, enzymolysis for 1h, inactivate enzyme (temperature 100°C, time 20min); after the above enzymolysis solution is cooled to 60°C, add neutral protease and papain, The amount of enzyme added is 0.3g / 100mL, the compound ratio is 1:3, the enzymolysis is 1h, the enzyme is inactivated (temperature 100°C, time 30min), centrifuged (speed 6000rpm, time 15min), the chelation rate is measured, and the quantitative enzymolysis solution is frozen Dried to obtain a grade I laver chelated manganese salt with a chelation rate of 30% (±5%), which was recorded as Experiment No. 3.

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Abstract

The invention relates to a nori small peptide chelate manganese compound and a preparation method as well as application thereof. The preparation method comprises the following steps: adding nori into water, adding a proper amount of water-soluble inorganic manganese salt, sufficiently stirring, and adding cellulase and pectinase for composite enzymatic hydrolysis, thus obtaining mixed liquor containing nori protein; adding neutral protease and papain into the mixed liquor containing the nori protein for secondary composite enzymatic hydrolysis, and centrifuging to obtain nori protein small peptide enzymatic hydrolysate; taking the nori protein small peptide enzymatic hydrolysate, stirring and oscillating, centrifuging, measuring a chelation late until the chelation is not changed, and freeze-drying. The prepared nori small peptide chelate manganese compound serves as a pig feed additive with addition amount accounting for 0.1 to 1 percent of the total mass of pig feed. Compared with the prior art, the low-value nori is taken as a protein raw material, and the prepared nori small peptide chelate manganese compound as the pig feed additive can be used to improve the palatability of the feed, effectively increase feed intake of pigs, increase daily gain and enhance the immunocompetence of the pigs.

Description

technical field [0001] The invention belongs to the technical field of pig feed additives, and relates to a laver small peptide chelated manganese compound and a preparation method and application thereof. Background technique [0002] Laver is cultivated in large quantities along the coast of China, and low-value laver is sold at a very low price because of its poor taste. However, its protein content is about 25%, and it also contains polysaccharides, agar, VC, and various minerals. At present, the main utilization of low-value laver is to make seaweed snack food, and there are few deep-processed products. It is particularly urgent and necessary to continuously carry out deep processing and comprehensive utilization of low-value laver. [0003] The main nutritional and physiological role of the trace element manganese (Mn) is as an enzyme activator or component in the metabolism of carbohydrates, lipids, proteins and cholesterol. Manganese is a component of several enzym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K10/14A23K20/147A23K20/20
Inventor 李同刚周通杨伟春温庆琪周盛昌肖丽萍施建成吴有林
Owner GUANGZHOU AONONG BIOTECH
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