Probiotic microcapsule taking protein aggregate-polysaccharide as wall material and preparation method thereof

A technology of protein aggregates and probiotics, which is used in food forming, food ingredients as encapsulants, food science, etc., can solve problems such as affecting application and instability of natural proteins, and achieve good acid resistance, enteric solubility, and survival. The effect of high rate and uniform particle size distribution

Active Publication Date: 2017-05-31
SHENYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Proteins and polysaccharides are important components of food, and they are also widely used in food systems. Natural protein itself will produce some unfavorable properties and instability during processing, which will affect its application

Method used

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  • Probiotic microcapsule taking protein aggregate-polysaccharide as wall material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Configuration of microcapsule wall material solution: rice bran protein solution with a concentration of 15%;

[0024] Activation of strains and their cultivation: the lyophilized powder of lactic acid bacteria preserved in the laboratory was added to an aqueous solution of 11% skim milk, and subjected to anaerobic constant temperature cultivation at 37° C. for 24 hours, and the skim milk solidified. Dilute 0.1mL curd with sterile water to prepare a suspension containing lactic acid bacteria. Separation of the bacterial suspension by streaking on a plate, anaerobic culture at 37°C for 24 hours, and continuous passage for 2-3 times in this way to fully activate it. Inoculate the activated lactic acid bacteria liquid into MRSC liquid medium, and after anaerobic constant temperature cultivation at 37°C to the logarithmic phase, centrifuge the obtained bacterial liquid at 4°C and 8000rmp for 10min, discard the supernatant, and collect the bacteria body;

[0025] Preparati...

Embodiment 2

[0029] Microcapsule preparation method is the same as example 1, change microcapsule wall material solution: rice bran protein aggregate solution

[0030] Preparation of rice bran protein aggregate solution: Dissolve a certain amount of rice bran protein in distilled water at a concentration of 15% (w / v) and stir for 2 hours to ensure that the rice bran protein can be completely hydrolyzed, adjust the pH of the rice bran protein solution to 2, and centrifuge Finally, the supernatant was collected, and then heated and denatured in a water bath at 90°C for 2 hours, and then cooled rapidly in an ice bath to prevent the growth of aggregates, forming a rice bran protein aggregate solution.

[0031] The embedding rate of the obtained lactic acid bacteria microcapsules reaches 75% + 1.1%. 12 CFU / g.

Embodiment 3

[0033] Microcapsule preparation method is the same as example 1, change microcapsule wall material solution: the solution+polysaccharide solution of rice bran protein aggregate (gum arabic, dextran, chitosan)

[0034]Preparation of rice bran protein aggregate solution: Dissolve a certain amount of rice bran protein in distilled water at a concentration of 15% (w / v) and stir for 2 hours to ensure that the rice bran protein can be completely hydrolyzed, adjust the pH of the rice bran protein solution to 2, and centrifuge Finally, collect the supernatant, then heat and denature it in a water bath at 90°C for 2 hours, take it out and put it in an ice bath to cool it rapidly to prevent the growth of aggregates, and the formed rice bran protein aggregate solution is ready for use;

[0035] The configuration of the microcapsule wall material solution: add 2% polysaccharide solution dropwise to the prepared rice bran protein aggregate solution according to the ratio (polysaccharide sol...

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Abstract

The invention provides a probiotic microcapsule taking protein aggregate-polysaccharide as a wall material and a preparation method thereof. The preparation method comprises the following steps: enabling rice bran protein to form aggregate at low pH, mixing with polysaccharide solution in a certain volume ratio of (1:1) to (10:1), adding probiotic bacterial suspension and right amount of an emulsifying agent (Tween-80) into the formed mixed solution, lightly and uniformly stirring, forming stable emulsion at the moment, adding 1% acetic acid solution into the mixed solution while stirring continuously, so that final pH of the mixed solution is 3.8-4.0, finally adding a curing agent (gallic acid) for curing a microcapsule, and then washing with distilled water, so that a probiotic microcapsule product is obtained finally. The probiotic microcapsule provided by the invention has the advantages that the aim of crosslinking can be achieved only by controlling ratio of the protein aggregate to the polysaccharide and crosslinking time, and the prepared probiotic microcapsule can improve resistance on gastric acid, can be released in intestinal tract and absorbed by human body finally and has good property.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a probiotic microcapsule product with rice bran protein aggregate-polysaccharide composite gel as wall material and a preparation method. Background technique [0002] Microcapsule technology is a technology that embeds solid, liquid or gaseous substances that are sensitive to the surrounding environment in capsules and can release them in a controlled manner in a specific environment. The size of microcapsule particles generally varies due to different processing techniques, and the particle size range is generally 0.1-1000um. Microcapsules are divided into two parts: wall material and core material. The material embedded in the microcapsule is called core material, and the material embedded outside is called wall material. Wall material is generally natural polymer material, semi-synthetic polymer material materials, fully synthetic polymer materials and inorganic...

Claims

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Application Information

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IPC IPC(8): A23P10/30A23L33/135A23L33/125A23L33/185
CPCA23V2002/00A23V2200/224A23V2250/5482
Inventor 肖志刚王霞高育哲王鹏李凡姝张一凡杨舒崔晓彤张文蕾
Owner SHENYANG NORMAL UNIV
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