Preparation method and detection method of carcino-embryonic antigen electrochemical immunosensor

An immunosensor and carcinoembryonic antigen technology, applied in the field of immunosensors, can solve the problems of unstable electrochemical response, complex detection steps, wrong detection results, etc., to eliminate the interference of dissolved oxygen, high selective permeability, and low cost. Effect
CN106770568AInactive Publication Date: 2017-05-31CHONGQING UNIV OF ARTS & SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHONGQING UNIV OF ARTS & SCI
Publication Date
2017-05-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of immunosensor, and more specifically discloses a preparation method and a detection method of a carcino-embryonic antigen (CEA) electrochemical immunosensor. The preparation method comprises following steps: poly o-phenylenediamine nanoparticles are dispersed in water via ultrasonic oscillation, and an obtained poly o-phenylenediamine nanoparticle solution is stored at room temperature; a glassy carbon electrode is modified with glutaraldehyde, and the glassy carbon electrode is immersed in a mixed solution composed of a HRP labeled CEA antibody solution and the poly o-phenylenediamine nanoparticle solution so as to obtain a poly o-phenylenediamine nanoparticle / HRP labeled CEA antibody modified electrode; solutions with different concentrations are prepared from CEA standard samples, and are taken as test solutions; an analyte is subjected to differential pulse voltammetry scanning in a H2O2 and oxygen-free PBS mixed solution; electrochemical reaction is carried out so as to establish the quantitative relationship of electrochemical current strength with the CEA solution concentration. The carcino-embryonic antigen electrochemical immunosensor is high in sensitivity; results are accurate and reliable; cost is low; detection is rapid; the carcino-embryonic antigen electrochemical immunosensor is convenient to use; and the preparation process is extremely simple.
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Description

technical field

[0001] The invention relates to the field of immunosensors, in particular to a preparation method and detection method of a carcinoembryonic antigen electrochemical immunosensor. Background technique

[0002] Carcinoembryonic antigen (CEA) is a glycoprotein with extremely large molecular weight, which is often used as a tumor marker. The detection of CEA concentration can not only be used in the diagnosis of tumors in the gastrointestinal tract and respiratory tract, but also can indicate the nature of the tumor by detecting the content and amount of carcinoembryonic antigen in the human body, so as to diagnose the tumor, analyze the course of the disease and guide clinical treatment. play an auxiliary role. Therefore, the determination of CEA concentration has great significance for clinical tumor diagnosis. The most common analytical methods for quantitative detection of CEA include fluorescent immunoassay, enzyme-linked immunosorbent assay, electrochemic...

Claims

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