Baby diaper with antibacterial and damp-proof functions
A technology of diapers and antimicrobial peptides, which is applied in applications, household appliances, and other household appliances. It can solve the problems of rough water absorption of diapers, skin irritation of newborns, and unsuitability for promotion and use, so as to facilitate large-scale promotion and use. Improves dryness and increases air flow
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Embodiment 1
[0018] The preparation of embodiment 1 antimicrobial peptide non-woven fabric
[0019] The invention utilizes a polypeptide synthesizer to synthesize antibacterial peptides SEQ ID NO: 1-11 by a solid-phase synthesis method. The specific methods are not described here one by one, as they already belong to the prior art.
[0020] Get the antimicrobial peptide 0.01 gram shown in SEQ ID NO: 1 and dissolve in 50 grams of polyurethane solution that has been diluted to 1.7%, then the non-woven fabric (the preliminary work of punching and increasing bumps has been done in the fabric ( The material of the bump is a food-grade PVC material), which is also operable in the prior art) and immersed in the above-mentioned solution, with a bath ratio of 1:50, soaked at room temperature for 10 minutes, dipped twice and rolled, and the excess ratio was 100, and then Pre-bake at 60°C for 20 minutes, bake at 90°C for 20 minutes, soak in water for 10 minutes, and dry to obtain a non-woven fabric ...
Embodiment 2
[0024] Embodiment 2 antibacterial detection
[0025] Antibacterial test method, take the coated fabrics and uncoated fabrics containing antimicrobial peptides with a diameter of 1 cm, sterilize with ultraviolet radiation (irradiation time is 30 minutes), infiltrate with sterile water; put the samples into sterile cell culture respectively dish, then add 50 microliters of bacterial culture solution (10 6 CFU / ml), and cultivated in a 37°C incubator for 2 hours; add 1 ml of phosphate buffer solution to each dish, and wash with ultrasonic waves ((64kHz, 2 minutes), then take 50 microliters of gradient dilution each, and mix 50 Add microliters of different dilutions of bacterial buffer solution to the petri dish, spread the bacterial solution evenly on the solid agar medium with a coating rod, and place it in a 37°C incubator for 24 hours; according to the solid agar medium The number of colonies is used to calculate the antibacterial ability of the sample. The plate with 200 sing...
Embodiment 3
[0029] Example 3 Antimicrobial Peptide Safety Verification
[0030]The antimicrobial peptides of the present invention were tested for hemolyticity using the conventional OD414 measurement method in the art, see (Table 2).
[0031] Table 2 Hemolytic properties of antimicrobial peptides
[0032] 500 μg / mL addition calculation OD 414nm value
[0033] Experiment shows (table 2): under the maximum concentration of use, the OD414nm of the hemolytic inspection of antimicrobial peptide of the present invention is all less than the hemolytic effect that 2% Triton X-100 causes, even the antimicrobial peptide of high concentration is almost also to erythrocyte. There is no destructive effect, that is, there is basically no hemolytic effect, so the use of the antimicrobial peptide of the present invention is relatively safe.
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