Bone repair material and its preparation method and application

A bone repair and structure-directing agent technology, applied in the field of bioengineering, can solve the problems of epidermal growth factor metabolism speed cannot complete the bone repair process, poor adsorption and release ability of biological macromolecules, poor differentiation ability in the direction of osteogenesis, etc., to achieve long-term effect Rapid bone repair process, enhance repair effect and speed, promote proliferation effect

Active Publication Date: 2020-03-31
杭州维叶莫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a bone repair material and its preparation method and application. This invention solves the problem that the existing bioactive glass has poor adsorption and release ability to biomacromolecules and insufficient osteoinductivity, which leads to long cell response time and osteogenesis. Poor directional differentiation ability, showing slow speed of bone regeneration and bone reconstruction; technical problems of epidermal growth factor metabolism in the process of bone repair is too fast to complete the bone repair process

Method used

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  • Bone repair material and its preparation method and application
  • Bone repair material and its preparation method and application
  • Bone repair material and its preparation method and application

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Effect test

Embodiment 1

[0050] Bioactive glass (MBG) was synthesized by a combined approach of template induction and self-assembly. 4.5g structure directing agent nonionic block copolymer PEO 20 -PPO 70 -PEO 20 (P123) was dissolved in 240 ml of water, the pH value of the solution was adjusted to 1 with HCl, 6.5 g of pore-enlarging agent 1,3,5-mesitylene (TMB) was added, stirred for 3 hours until uniform, and then 7 ml of orthosilicic acid was added Ethyl ester (TEOS), 3.65g Ca(NO 3 ) 2 4H 2 O calcium source, 2.5g triethyl phosphate (TEP), after stirring at 40°C for 24 hours, adjust the pH to 10 with concentrated ammonia water, and let it stand at 110°C for 50 hours to obtain a precipitate. The obtained precipitate was washed several times with deionized water until the pH was 7, and fired at 550° C. for 10 hours to obtain a bioactive glass material. The bioactive glass material was mixed with 3-aminopropyltriethoxysilane (APTES, the molecular formula is C 9 h 23 NO 3 Si,), mixed for 20 hour...

Embodiment 2

[0063] Bioactive glass (MBG) was synthesized by a combined approach of template induction and self-assembly. 6 g of structure directing agent nonionic block copolymer PEO 20 -PPO 70 -PEO 20 (P123) was dissolved in 240 ml of water, the pH value of the solution was adjusted to 1 with HCl, 10 g of pore-enlarging agent 1,3,5-mesitylene (TMB) was added, stirred at 40°C for 6 hours until uniform, and then 10 ml of Orthoethyl silicate (TEOS), 4.55g Ca(NO 3 ) 2 4H 2 O, 2.5g of triethyl phosphate (TEP), stirred at 45°C for 24 hours, adjusted the pH to 11 with concentrated ammonia water, and allowed to stand at 90°C for 56 hours to obtain a precipitate. The obtained precipitate was washed several times with deionized water until the pH was 7.4, and fired at 550° C. for 10 hours to obtain a bioactive glass material. The bioactive glass material was mixed with 3-aminopropyltriethoxysilane (APTES, the molecular formula is C 9 h 23 NO 3 Si,), mixed for 14 hours, baked at 150°C for ...

Embodiment 3

[0065] Bioactive glass (MBG) was synthesized by a combined approach of template induction and self-assembly. 4 g of structure directing agent nonionic block copolymer PEO 20 -PPO 70 -PEO 20 (P123) was dissolved in 240 ml of water, the pH of the solution was adjusted to 2 with HCl, 6 g of pore-enlarging agent 1,3,5-mesitylene (TMB) was added, stirred for 3 hours until uniform, and then 6 ml of ethyl orthosilicate was added Ester (TEOS), 2.75g calcium chloride, 2.5g triethyl phosphate (TEP), stirred at 50°C for 20 hours, adjusted the pH to 9 with concentrated ammonia water, and allowed to stand at 110°C for 80 hours to obtain a precipitate. The obtained precipitate was washed several times with deionized water until the pH was 7, and fired at 600° C. for 6 hours to obtain a bioactive glass material. The bioactive glass material was mixed with 3-aminopropyltriethoxysilane (APTES, the molecular formula is C 9 h 23 NO 3 Si,), mixed for 20 hours, baked at 80°C for 30 hours, wa...

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Abstract

The invention provides a bone repairing material, and a preparation method and application of the bone repairing material. The preparation method of the material comprises the steps: dissolving a structure directing agent and a pore-enlarging agent, adding a calcium source, a silicon source and a phosphorus source, adjusting a pH (potential of hydrogen) value to 9-11, placing for 56-80 h at 90-110 DEG C, baking for 6-10 h at 550-600 DEG C to form bioactivity glass, mixing the bioactivity glass and 3-aminopropyltriethoxysilane for 14-20 h, baking for 20-30 h at 80-150 DEG C, washing with chloroform, drying to form amino modified bioactivity glass, and incubating the amino modified bioactivity glass and a cell factor in a carbon dioxide incubator at 37 DEG C for 4-6 h to promote adsorption to form the bone repairing material. The material can promote osteoblast proliferation, osteogenesis direction differentiation and mineralization processes, has high cell compatibility and a good effect of promoting bone formation and has wide application prospects as the repairing material in a bone defect repairing process.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a bone repair material and its preparation method and application. Background technique [0002] Bioactive glass is a kind of porous material with a pore size between 2-50nm. It mainly contains silicon, calcium and phosphorus. It has a very high specific surface area, regular and orderly pore structure, narrow pore size distribution, and continuously adjustable pore size. features. These characteristics make it widely used in the adsorption and separation of macromolecules. The material has good biological activity, which can match the new bone formation with the degradation rate of the material. The dissolved ions can activate the expression of osteogenic genes, promote the formation, proliferation, differentiation and mineralization of extracellular matrix of osteoblasts, and play a role in bone formation. Induction. With good biocompatibility and biodegradability, it...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/12A61L27/10A61L27/56A61L27/54
CPCA61L27/10A61L27/12A61L27/54A61L27/56A61L2300/112A61L2300/414A61L2300/602A61L2430/02
Inventor 王晓燕高凯王干柳珑
Owner 杭州维叶莫生物科技有限公司
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