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Ratiometric fluorescent probe based on ion imprinting, preparation and application thereof

A ratiometric fluorescent probe and ion imprinting technology, which is applied in the direction of fluorescence/phosphorescence, luminescent materials, and material analysis through optical means, can solve the problems of unreported detection of cadmium ions, etc., and achieve rapid visual detection, convenience, excellent specificity, cheap effect

Inactive Publication Date: 2017-06-13
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To the best of our knowledge, no ion imprint-based quantum dot ratiometric fluorescent probes have been reported for the detection of cadmium ions.

Method used

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  • Ratiometric fluorescent probe based on ion imprinting, preparation and application thereof
  • Ratiometric fluorescent probe based on ion imprinting, preparation and application thereof
  • Ratiometric fluorescent probe based on ion imprinting, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Preparation of red quantum dots

[0037] 0.2284g cadmium chloride (CdCl 2 2.5H 2 O) Add it to 500mL deionized water, pass nitrogen gas for 20min, then add 0.1mL mercaptopropionic acid, then adjust the pH to 11.00 with 1M aqueous sodium hydroxide solution, then continue to add 0.538g sodium citrate (containing crystal water), 0.0444g Sodium tellurite, 0.2g sodium borohydride, and finally heated to reflux. After refluxing for 8h, obtain a stable cadmium telluride quantum dot aqueous solution (i.e. red quantum dot solution) that emits red fluorescent mercaptopropionic acid, the emission peak is at 628 nanometers, and the concentration is 4.3 × 10 -6 mol / L.

[0038] Take 4mL of the synthesized red quantum dot solution, add 4mL of absolute ethanol, let it settle, centrifuge, wash the precipitate with absolute ethanol 1-2 times, and obtain 3 mg of cadmium telluride red quantum dots.

[0039] 2. Silica nanoparticles embedded with red quantum dots

[0040] Take 1mg of r...

Embodiment 2

[0044] 1. Preparation of red quantum dots

[0045] 0.2284g cadmium chloride (CdCl 2 2.5H 2 O) Add it to 500mL deionized water, pass nitrogen gas for 20min, then add 0.1mL mercaptopropionic acid, then adjust the pH to 11.00 with 1M aqueous sodium hydroxide solution, then continue to add 0.538g sodium citrate (containing crystal water), 0.0444g Sodium tellurite, 0.2g sodium borohydride, and finally heated to reflux. After refluxing for 8h, obtain a stable cadmium telluride quantum dot aqueous solution (i.e. red quantum dot solution) that emits red fluorescent mercaptopropionic acid, the emission peak is at 628 nanometers, and the concentration is 4.3 × 10 -6 mol / L.

[0046] Take 4mL of the synthesized red quantum dot solution, add 4mL of absolute ethanol, let it settle, centrifuge, wash the precipitate with absolute ethanol 1-2 times, and obtain 3 mg of cadmium telluride red quantum dots.

[0047] 2. Silica nanoparticles embedded with red quantum dots

[0048] Take 1mg of r...

Embodiment 3

[0052] 1. Preparation of red quantum dots

[0053] 0.4568g cadmium chloride (CdCl 2 2.5H 2 O) Add it to 500mL deionized water, pass nitrogen gas for 20min, then add 0.2mL mercaptopropionic acid, then adjust the pH to 11.00 with 1M aqueous sodium hydroxide solution, then continue to add 1.076g sodium citrate (containing crystal water), 0.0888g Sodium tellurite, 0.4g sodium borohydride, and finally heated to reflux. After refluxing for 8h, obtain a stable cadmium telluride quantum dot aqueous solution (i.e. red quantum dot solution) that emits red fluorescent mercaptopropionic acid, the emission peak is at 628 nanometers, and the concentration is 6.5 × 10 -6 mol / L.

[0054] Take 2mL of the synthesized red quantum dot solution, add 2mL of absolute ethanol, let it settle, centrifuge, wash the precipitate with absolute ethanol 1-2 times, and obtain 2.8 mg of cadmium telluride red quantum dots.

[0055] 2. Silica nanoparticles embedded with red quantum dots

[0056] Take 1mg of...

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Abstract

The invention discloses a ratiometric fluorescent probe based on ion imprinting, preparation and application thereof. The fluorescent probe takes a red quantum dot as the core and is embedded in a silicon dioxide nanoparticle, then amination modification is performed on the surface of the silicon dioxide nanoparticle, and the amination modified surface is covalently coupled with a green quantum dot to obtain the ratiometric fluorescent probe based on ion imprinting. The ratiometric fluorescent probe detects metal ions based on ratiometric ion imprinting of quantum dots, has excellent specificity, good stability, simple preparation, cheap price and the like, greatly shortens the reaction time, reduces detection of cadmium ions to 25nM, and provides convenience for visualized quick detection.

Description

[0001] (1) Technical field [0002] The invention relates to a preparation and detection method of a rapid visual ratio fluorescent probe for the detection of cadmium ions in water. [0003] (2) Background technology [0004] Cadmium is a toxic heavy metal that is ubiquitous in industry, polluted agricultural products and our daily life. When the environment is polluted by cadmium, cadmium can be enriched in organisms. Cadmium can enter the human body through the food chain, combine with sulfhydryl groups on proteins to form cadmium-sulfur protein, selectively accumulate in the liver and kidney, and cause a series of acute and chronic diseases, such as osteoporosis and lung cancer. Therefore, we urgently need to develop a convenient and feasible method to detect Cd 2+ . Traditional CDs 2+ Detection methods, such as atomic absorption spectroscopy, inductively coupled plasma mass spectrometry, particle-induced X-ray fluorescence analysis, chromatography and voltammetry, have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/02G01N21/64
CPCC09K11/025C09K11/883G01N21/643
Inventor 汪晶彭晓蒋晨星王力耕胡军
Owner ZHEJIANG UNIV OF TECH
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