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A Strain of Paracoccus versicolor and Its Application

A paracoccus and bacterial strain technology, applied in the direction of bacteria, gas fuel, waste fuel, etc., can solve the problems of high economic cost, lower biogas energy efficiency, and easy to be limited by light sources, and achieve the effect of avoiding potential safety hazards

Active Publication Date: 2019-11-08
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photoautotrophic microorganisms remove H 2 S process, although a higher H 2 S removal rate and no safety hazards under anaerobic conditions, etc., but requires a large amount of radiant energy, its economic cost is high, and it is easily limited by the light source
Therefore, chemoautotrophic microorganisms become the biological removal of biogas H 2 A better choice for S. Under the condition of aerobic presence, chemoautotrophic microorganisms use oxygen as an electron acceptor, but the regulation of oxygen is difficult, and there is a danger of explosion when there is too much oxygen supply. At the same time, nitrogen and other gases will be introduced at the same time Inert gas, which reduces the concentration of methane in the biogas and reduces the energy efficiency of the biogas

Method used

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  • A Strain of Paracoccus versicolor and Its Application
  • A Strain of Paracoccus versicolor and Its Application
  • A Strain of Paracoccus versicolor and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Isolation and purification of bacterial strains

[0032] The anaerobic sludge was taken from the third sewage treatment plant in Chengdu, Sichuan. The collected sludge was acclimatized with a medium enriched with sulfur compounds under anaerobic conditions, and then serially diluted after 7 days, starting from 10 -2 、10 -4 、10 -6 、10 -8 、10 -10 Draw 0.1ml of the dilution and spread it on the separation medium plate, and a single colony scattered on the surface of the plate appears, pick a single colony, repeat the above operation several times, and finally obtain a solution that can remove H from biogas using nitrate as an electron acceptor. 2 Purified strains of S.

[0033] The enrichment medium is: Na 2 S 2 o 3 ·5H 2 O 5g, KNO 3 4g, KH 2 PO 4 2g, NaHCO 3 1g, MgCl 2 ·6H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, pH value 7.0, tap water 1000mL. 121°C, 30min high temperature sterilization. Separation medium: Na 2 S 2 o 3 ·5H 2 O 10g, KNO 3 4g, KH 2 P...

Embodiment 2

[0045] The present invention paracoccus fimutans strain CGMCC No.13321 is applied to the H in biogas 2 S removed.

[0046] 1. Preparation of culture medium and inoculation solution

[0047] Solid activation medium: Na 2 S 2 o 3 ·5H 2 O 10g, KNO 3 4g, KH 2 PO 4 2g, NaHCO 3 1g, MgCl 2 ·6H 2 O0.5g, FeSO 4 ·7H 2 O 0.01g, 20g agar, 1000mL distilled water, pH7.0-7.5, 121℃, 30min high temperature sterilization. Liquid fermentation medium: Na 2 S 2 o 3 ·5H 2 O 10g, KNO 3 4g, KH 2 PO 4 2g, NaHCO 3 1g, 1000ml tap water, pH7.0-7.5, 121℃, 30min high temperature sterilization treatment. Desulfurization medium: KNO 3 4g, KH 2 PO 4 1g, NaHCO 3 1g, 1000ml tap water, pH7.0-7.5, 121℃, 30min high temperature sterilization treatment.

[0048] Inoculate Paracoccus mutans strain CGMCC No.13321 into the solid activation medium, and activate the culture for 1 day to obtain the activated strain, which is ready for use; put the activated strain into the liquid ferment...

Embodiment 3

[0055] Paracoccus mutans strain CGMCC No.13321 for biogas H 2 S removal, the same as Example 3 will not be repeated, the difference lies in the treatment conditions from the inoculated bacteria solution to the desulfurization reactor.

[0056] The treatment temperature is 20°C, and the H in the biogas is continuously measured 2 The removal rate of S is shown in Table 2 for the measurement results.

[0057] Table 2 Biogas H 2 S removal rate

[0058]

[0059] It can be seen from the above table that the temperature is 20°C, and the biogas H 2 The concentration of S was significantly lower than that of the control group, in which the biogas H 2 The removal rate of S was only 6.2%, while the experimental group biogas H 2 The removal rate of S is as high as 77.1%. Compared with the temperature condition of 30℃, although Paracoccus mutans strain CGMCC No.13321 2 The removal rate of S decreased, but still showed good H 2 S removal effect.

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PUM

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Abstract

The invention discloses a strain of Paracoccus versutus 2# CGMCC No. 13321 and its use of nitrate as an electron acceptor to oxidatively remove hydrogen sulfide (H 2 S) application. Gram staining of this strain is negative, the cells of the strain are short rod-shaped and small; the colony is white raised, the edges are neat and smooth, and the surface is moist; the strain is intolerant to salt and cannot use citrate; when nitrate, nitrite or oxidation In the presence of nitrogen, they can be used as electron acceptors for anaerobic growth. After inoculation of Paracoccus versicolor strain CGMCC No.13321 into the desulfurization reactor, nitrate can be used as an electron acceptor for H in the reactor. 2 S undergoes biological oxidation, and grows and reproduces to reach H in the biogas 2 The purpose of S removal effectively avoids the traditional use of oxygen as an electron acceptor for biogas H 2 The safety hazard brought by the introduction of methane and oxygen in the removal of S has a negative impact on the H in the biogas. 2 The removal rate of S is as high as 93.8%.

Description

technical field [0001] The present invention relates to a paracoccus bacterial strain, in particular to a method that can utilize nitrate as an electron acceptor to oxidize and remove hydrogen sulfide (H 2 S) Paracoccus mutans strain. Background technique [0002] Biogas is an emerging clean and renewable energy. Anaerobic fermentation technology with biogas as the target product has been more and more widely used because of its good energy and environmental benefits. It will be of great significance to alleviate my country's energy crisis and protect the environment. Biogas is a kind of biofuel produced by anaerobic fermentation of biological raw materials. The biological raw materials used for anaerobic fermentation of biogas mainly include livestock and poultry manure, straw, energy plants, domestic garbage, organic wastewater and other wastes. At present, the main raw materials for biogas fermentation in my country are manure and sewage from livestock and poultry farms....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C10L3/10C12R1/01
CPCC10L3/103C12N1/20C12N1/205C12R2001/01Y02E50/30
Inventor 闫志英唐维林肖莉凡刘晓风袁月祥李东姬高升房俊楠
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S