Preparation method of L-aspartic acid (2, 3, 3-D3) by enzymatic catalyst

Abstract

The invention relates to a preparation method of L-aspartic acid (2, 3, 3-D3) by enzymatic catalyst, wherein the preparation method includes steps of (1), orderly placing L-aspartic acid or its salt, alpha- oxoglutarate (or alpha- sodium oxoglutarate) in a three-opening bottle; replacing an oil pump for three times under the argon atmosphere, and then adding deuterium oxide under the argon atmosphere; adjusting the pH value of the reaction liquid to 8.0-9.0 by sodium deuteroxide solution / deuterium lithium carbonate water solution / anhydrous triethylamine; preserving temperature at 32-42 DEG C; then adding glutamic oxalacetic transaminase 20-10 OU, and preserving temperature for 1-5 days; (2) filtering the reaction liquid by a millipore filter, depressurizing and steaming off the major deuterium oxide and recycling the deuterium oxide; then adjusting pH value to 2.5-3.0; placing, cooling and crystalizing the reaction liquid; filtering and drying the reaction liquid; acquiring the product. The preparation method has the advantages of using the low-price amino acid as the substrate and deuterium oxide as the deuterium source, thus the chiral L-aspartic acid (2, 3, 3-D3) product is directly acquired.

Description

technical field [0001] The present invention relates to a kind of synthetic L-aspartic acid (2,3,3-D 3 ) method, especially relate to a kind of directly using cheap L-aspartic acid, deuterium water as raw material synthesis L-aspartic acid (2,3,3-D 3 )Methods. Background technique [0002] L-Aspartic Acid (2,3,3-D 3 ) is an important stable isotope-labeled amino acid, which has a wide range of applications in medicine, biology, pharmacy and chemistry. L-Aspartic Acid (2,3,3-D 3 ) can be used to synthesize stable isotope-labeled polypeptide compounds, and is of great significance in the biological metabolism of polypeptides, disease diagnosis and treatment, and the mechanism and role of amino acids in cell culture. At the same time, L-aspartic acid (2,3,3-D 3 ) is also an important raw material for the synthesis of other deuterated L-amino acids, such as L-lysine (2,3,3-D 3 ), L-leucine (2,3,3-D 3 ), L-arginine (2,3,3-D 3 ), etc.; or important raw materials for synthe...

AI Technical Summary

Problems solved by technology

However, this method has long steps, and the synthesis cost of chiral synthons is high, while achiral synthons need to be disassembled. At the same time, too many inorganic salts need to be purified with resin, and the process is cumbersome.

[0008] In summary, the synthesis of L-aspartic acid (2,3,3-D 3 ) methods are relatively cumbersome, or need to be resolved, or the comprehensive yield is seriously low, and need to be purified with resin

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