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Method for detecting uric acid in body fluid by quantum dot

A quantum dot solution, quantum dot technology, applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of expensive reagents and complicated operation, and achieve the effects of high sensitivity, good accuracy and high specificity

Inactive Publication Date: 2017-06-20
JINZHOU MEDICAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Compared with non-enzymatic methods such as high performance liquid chromatography and capillary electrophoresis, the enzymatic method based on uric acid oxidase has high specificity, but due to its complicated operation and expensive reagents, ascorbate oxidase should be added in the process to reduce Interference from reducing substances such as ascorbic acid
Therefore, there are still many defects

Method used

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Effect test

Embodiment 1

[0027] (1) 10mg of methyl cadmium [(CH 3 ) 2 Cd] and 10mg TOPSe (trioctylphosphine selenium) were quickly injected into the vigorously stirred trioctylphosphine oxide (TOPO) at 350°C; then rapidly cooled to 250°C, and then slowly heated to 280°C; excess methanol was added, passed Centrifuge to obtain CdSe nanoparticles; add 8mg zinc stearate, 12mg hexamethyldisilathane [(TMS) in TOPO 2 S] Zn and CdSe nanoparticles, add microwave oven to heat at high temperature, after the solution is naturally cooled, filter with medium-speed filter paper to remove insoluble black precipitate, centrifuge to remove large particles, collect supernatant and pour it into dialysis bag for dialysis; the product after dialysis Perform vacuum rotary evaporation to solid state to obtain CdSe / ZnS quantum dots;

[0028] (2) Mix and dissolve 10 mL of poly(tert-butylacrylate-ethylacrylate-methacrylic acid) triblock copolymer and 10 mL of octylamine in dimethyl sulfoxide, add carbodiimide to react for 6 h...

Embodiment 2

[0032]Example 2 sample recovery experiment

[0033] In order to investigate the feasibility of this method, the content of uric acid in human urine samples was determined by standard addition and recovery experiments.

[0034] In order to obtain a sample with a suitable concentration, it needs to be diluted 2000 times with secondary water before determination. The measurement results are shown in Table 1. The results show that the relative deviation of the method is less than 2.23%, and the recovery rate is between 97% and 103%, indicating that the method has good precision and accuracy.

[0035] Table 1 Sample recovery experiment

[0036]

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Abstract

The invention relates to a method for detecting uric acid in body fluid by a quantum dot, and relates to a method for detecting the content of uric acid by adopting a water soluble CdSe / ZnS quantum dot. By utilizing the characteristics that hydrogen peroxide can oxidize catechol under the action of HRP (horseradish peroxidase), and the oxidized product can allow the fluorescence of the quantum dot to be efficiently quenched, the uric acid concentration in a sample to be tested is obtained. The method provided by the invention has the characteristics of high sensitivity, low toxicity, high specificity, and good accuracy.

Description

technical field [0001] The invention relates to a method for detecting uric acid in body fluid, in particular to a method for detecting uric acid in body fluid by using quantum dots. Background technique [0002] Quantum dots are also called fluorescent semiconductor nanoparticles. Quantum dots can emit fluorescence after being irradiated by excitation light, and can be combined with biological macromolecules, which has strong biocompatibility. Compared with traditional fluorescent dyes, quantum dots have tunable emission wavelengths, and quantum dots with different emission spectra can be obtained by adjusting their particle size. At the same time, quantum dots have a large Stokes shift and a symmetrical fluorescence spectrum, which can effectively reduce the influence of excitation light on emitted light, thereby reducing system complexity. [0003] In recent years, with the improvement of living standards, the incidence of hyperuricemia and gout has been increasing year ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 王冠男梅晰凡金丽陈书睿高巍
Owner JINZHOU MEDICAL UNIV
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