Application of Bacillus megaterium to treatment of municipal sludge and method for producing biological organic fertilizer from Bacillus megaterium

A technology of Bacillus megaterium and municipal sludge, which is applied in the field of bio-organic fertilizer production, can solve the problems of late start, difficulty in finding sludge disposal methods for treatment plants, sludge disposal sites, and low bacterial survival rate.

Inactive Publication Date: 2017-06-23
SHANGHAI LVLE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the late start of sludge treatment and disposal in my country, its own treatment technology and equipment are relatively backward, and many cities have not incorporated sludge disposal sites into the overall urban planning, making it difficult for many treatment plants to find suitable sludge disposal methods and sludge disposal sites
[0004] Although it has been reported that bacteria are used to treat municipal sludge, due to the complex composition of municipal sludge, the survival rate of bacteria is low and the treatment effect is not good. The treatment method is of great significance

Method used

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  • Application of Bacillus megaterium to treatment of municipal sludge and method for producing biological organic fertilizer from Bacillus megaterium
  • Application of Bacillus megaterium to treatment of municipal sludge and method for producing biological organic fertilizer from Bacillus megaterium
  • Application of Bacillus megaterium to treatment of municipal sludge and method for producing biological organic fertilizer from Bacillus megaterium

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 handles the method for municipal sludge

[0033] The concrete steps of the method that Bacillus megaterium of the present invention is used for processing municipal sludge are as follows:

[0034] (1) Pre-treatment of sludge samples:

[0035] Take municipal sludge and use tap water to adjust the solid content of the sludge to 1%, add defoamer, the amount of defoamer added is 3g / L, adjust the pH of the sludge sample to 7-8, and test in the seed tank and fermentation tank respectively Sterilize at 0.1MPa pressure and 121°C for 45 minutes, cool to 30°C for later use;

[0036] (2) Bacillus megaterium shake flask culture:

[0037] Inoculate Bacillus megaterium into a Erlenmeyer flask equipped with fermentation medium according to the inoculation amount of 5%, and carry out shake flask culture. The culture conditions are: temperature 25°C, rotation speed 300r / min, culture time 10 hours;

[0038] (3) Seed tank fermentation and fermenter fermentation:

[0039]I...

Embodiment 2

[0043] Embodiment 2 handles the method for municipal sludge

[0044] The concrete steps of the method that Bacillus megaterium of the present invention is used for processing municipal sludge are as follows:

[0045] (1) Pre-treatment of sludge samples:

[0046] Take municipal sludge and use tap water to adjust the solid content of the sludge to 5%, add defoamer, the amount of defoamer added is 1g / L, adjust the pH of the sludge sample to 7-8, and test in the seed tank and fermentation tank respectively Sterilize at 0.1MPa pressure and 121°C for 60 minutes, cool to 30°C for later use;

[0047] (2) Bacillus megaterium shake flask culture:

[0048] Inoculate Bacillus megaterium into a Erlenmeyer flask equipped with fermentation medium according to the inoculation amount of 1%, and carry out shake flask culture. The culture conditions are: temperature 35°C, rotation speed 200r / min, culture time 20 hours;

[0049] (3) Seed tank fermentation and fermenter fermentation:

[0050] ...

Embodiment 3

[0054] Embodiment 3 handles the method for municipal sludge

[0055] (1) Pre-treatment of sludge samples

[0056] Sludge from a sewage treatment plant in Shanghai was randomly sampled to determine the basic physical and chemical properties of the sludge. The results are shown in Table 1.

[0057] Table 1 Physical and chemical properties of sludge

[0058]

[0059] Use tap water to adjust the solid content of the sludge to 3.0%, add defoamer, the amount of defoamer added is 2.0g / L, adjust the pH of the sludge sample to about 7.0-8.0, and pass 0.1MPa in the seed tank and fermentation tank respectively. Sterilize under pressure and temperature of 121°C for 45-60 minutes, cool to 30°C for later use, and the pH of the sludge sample after sterilization is about 7.0-8.0.

[0060] (2) Bacillus megaterium shake flask culture

[0061] The Bacillus megaterium was inoculated into multiple Erlenmeyer flasks containing fermentation medium according to the inoculum amount of 3%, and cu...

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Abstract

The invention discloses an application of Bacillus megaterium to treatment of municipal sludge and a method for producing a biological organic fertilizer from Bacillus megaterium. The preservation number of Bacillus megaterium is ACCC10010, the organism is classified and named as Bacillus megaterium, and Bacillus megaterium is preserved in Agricultural Culture Collection of China. The method is used for treating municipal sludge, sludge samples before and after fermentation are determined, organic pollutants in the sludge are basically and completely degraded, concentration of heavy metal ions is substantially reduced, and the purpose for harmless treatment of the municipal sludge is realized. The biological organic fertilizer produced from fermentation liquor which is generated in the sludge treatment process and used as a raw material is detected, the effective living bacteria number of Bacillus megaterium in a finished product is 1.76x10<8>MPN / g, mixed bacterium rate is lower than 10%, pH is 6.51, and the results accord with a biological organic fertilizer standard of department of agriculture (NY / T 884-2004).

Description

technical field [0001] The invention relates to the technical field of municipal sludge treatment, in particular to an application of Bacillus megaterium in treating municipal sludge and a method for producing bio-organic fertilizer. Background technique [0002] Municipal sludge is solid waste produced during sewage treatment. With the development of sewage treatment in our country, the total water treatment volume and treatment degree of sewage plants will continue to expand and improve, and the amount of sludge produced will also increase day by day. In addition to a large amount of organic matter and rich nitrogen, phosphorus and other nutrients, sludge also contains toxic and harmful components such as heavy metals, pathogenic bacteria and parasites. In order to prevent secondary pollution caused by sludge and ensure the normal operation and treatment effect of sewage treatment plants, the issue of sludge treatment and disposal has become increasingly prominent in urba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/02C12N1/20C05F15/00C12R1/11
CPCC02F11/02C02F2303/06C05F5/008C12N1/20C05F11/00
Inventor 唐良梁
Owner SHANGHAI LVLE BIO TECH
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