Phytophthora nicotianae fermentation liquid pathogenic active ingredient and preparation method thereof

Abstract

The invention relates to a phytophthora nicotianae fermentation liquid pathogenic active ingredient and a preparation method thereof. The method comprises the following steps that (1) phytophthora nicotianae hyphae are subjected to shaking bed oscillation culture in a culture medium; sterile fermentation liquid is collected; centrifugation is performed; supernate is filtered and degermed for use; (2) the degermed phytophthora nicotianae sterile fermentation liquid obtained in the step (1) is subjected to membrane filtration separation through a microfiltration hollow cellulose membrane and an ultrafiltration hollow cellulose membrane to obtain clear and transparent ultrafiltration liquid; ultrafiltration filter liquid is fed into a column to be subjected to macroporous resin enrichment; then, ingredients adsorbed on the resin can be flushed down by ethyl alcohol; ethyl alcohol elution liquid, i.e., the phytophthora nicotianae fermentation liquid pathogenic active ingredient, is obtained. The phytophthora nicotianae fermentation liquid pathogenic active ingredient and the preparation method have the advantages that the operation is simple; the result is reliable; the pathogenic capability of the phytophthora nicotianae fermentation liquid pathogenic active ingredient is high; the stability is high; the phytophthora nicotianae fermentation liquid pathogenic active ingredient can be efficiently used for screening phytophthora nicotianae resistance varieties.

Description

technical field [0001] The invention belongs to the field of pathology, and in particular relates to a pathogenic active component of fermented liquor of tobacco black shank bacteria and a preparation method thereof. Background technique [0002] Tobacco black shank was first discovered in Java, Indonesia. It was first reported in 1950 that the disease occurred in the Huanghuai smoking area of ​​my country, and it has been harmful for a long time and the condition has become more and more serious. At present, except for the sporadic incidence of tobacco black shank in the northeast smoking areas, tobacco black shank disease is common in other smoking areas in the country, causing serious economic losses every year, second only to viral diseases. [0003] At present, the commonly used chemical agents for the prevention and treatment of tobacco black shank mainly include Ruifu mold (metalaxyl) wettable powder, Bordeaux mixture, thiophanate-methyl, dikexone wettable powder, an...

AI Technical Summary

Problems solved by technology

[0004] With the continuous development of the tobacco industry in the direction of green and pollution-free, the prevention and control of tobacco black shank only stays at a reasonable rotation as the main, ridge cultivation and chemical control as supplementary comprehensive prevention and control measures can no longer meet the needs of development

In biological control, searching for strains that antagonize tobacco black shank can also effectively control tobacco black shank to a certain extent, but only the disease resistance of tobacco varieties is the basis for disease control. A hot topic in the field

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