Gelatin sustained release microsphere loaded with growth factors and preparation method thereof

A technology of slow-release microspheres and growth factors, which is applied in the field of medicine, can solve the problems that there are no gelatin microsphere products, and achieve the effect of loading growth factors, good slow-release effect in vitro, convenient operation, and controlled release

Inactive Publication Date: 2017-07-07
GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because gelatin itself has sol-gel properties, gelatin microspheres can also be used as templates for prep...

Method used

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  • Gelatin sustained release microsphere loaded with growth factors and preparation method thereof
  • Gelatin sustained release microsphere loaded with growth factors and preparation method thereof
  • Gelatin sustained release microsphere loaded with growth factors and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of the gelatin slow-release microspheres loaded with growth factors of embodiment 1

[0049] (1) Preparation of blank gelatin microspheres

[0050] Accurately weigh 3.0 g of solid gelatin with an electronic balance, dissolve in 20 mL of 50°C distilled water, stir to dissolve, and make a gelatin solution with a mass fraction of 13%, and place it in a 50°C water bath for use.

[0051] Pour 100mL of liquid paraffin into the three-necked flask, then add 1.5mL of Span 80, stir evenly, and then place it in a constant temperature magnetic heating stirrer at 50°C for 10 minutes;

[0052] Adjust the speed of the agitator to 750-825r / min, add the prepared gelatin solution with a mass fraction of 15% dropwise into the three-necked bottle, add dropwise while stirring, and continue to stir and emulsify for 10 minutes after the dropwise addition, until the water-in-oil type is formed Emulsion;

[0053] Then quickly transfer the three-necked flask into an ice-water bath,...

Embodiment 2

[0065] Example 2 Gelatin slow-release microspheres loaded with growth factors for the relevant growth factor adsorption rate and loaded growth factor rate

[0066] The solvent extraction method (according to the relevant provisions of the second appendix XIXE of the "Chinese Pharmacopoeia") was used to detect with enzyme-linked immunosorbent assay (ELISA).

[0067] Specific steps: Take the EP tubes containing blank microspheres, add 100 μL of PG supernatant (the platelet composite growth factor prepared in step (3) of Example 1) to each EP tube, fully infiltrate and expand at 4°C for 24 hours Finally, the gelatin microspheres loaded with growth factors were prepared; the EP tube was centrifuged at 8090r / min for 15min, the entire supernatant (A) was sucked out, and PDGF-BB and TGF in the collected supernatant were detected by enzyme-linked immunosorbent assay (ELISA). -β, VEGF, IGF-1, bFGF, EGF content, in conjunction with the detection result of growth factor total amount in P...

Embodiment 3

[0073] Example 3 Gelatin slow-release microspheres loaded with growth factors for the detection of the slow-release rate of related growth factors

[0074] Use the ELISA kit to detect the sample of the gelatin slow-release microspheres loaded with growth factors collected in 1 to 7 days, the unadsorbed platelet composite growth factor sample in step (4) of embodiment 1, and the preparation of step (2) of embodiment 1 The platelet complex growth factor content in the platelet complex growth factor sample was calculated, the microsphere sustained release rate was calculated, and the in vitro sustained release curve was drawn. The in vitro sustained-release results are shown in Table 1, and the specific curve is as follows Figure 3-5 shown.

[0075] Table 1 ELISA detection results of VEGF sustained release

[0076]

[0077] It can be seen from the data in Example 3 that the prepared growth factor-loaded gelatin sustained-release microspheres have a good slow-release effect ...

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Abstract

The invention discloses a gelatin sustained release microsphere loaded with growth factors and a preparation method thereof. The gelatin sustained release microsphere loaded with the growth factors and the preparation method thereof take blood platelet compound growth factors as a packaging object, take gelatin microspheres as a carrier, and is prepared by freeze drying after infiltration and expansion. The prepared gelatin sustained release microsphere loaded with the growth factors can effectively use the compound growth factors, can achieve the effects of partially slow release and long lasting effects, can maintain everlasting biological activity, can promote the tissue regeneration and wound healing better, and can provide reference for the preliminary development of the gelatin sustained release microsphere lyophilized product loaded with the blood platelet compound growth factors. The preparation method provided by the invention is simple and is convenient to operate, the whole reaction process is mild in condition and the market prospect is broad.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to gelatin sustained-release microspheres loaded with growth factors and a preparation method thereof. Background technique [0002] Refractory wounds refer to wounds that cannot heal normally within the expected time, and are a type of wounds related to the site and host. The characteristics of refractory wounds and the difficulty of repair lie in the lack of a good blood supply in the local wounds, making it difficult to generate fresh blood vessels, and the surrounding tissues are repeatedly inflammatory and stimulated for a long time, resulting in fibrous tissue hyperplasia and scar tissue formation. Because the wound cannot heal for a long time, the defect of skin tissue is prone to liquefaction, infection and even necrosis, which often causes great psychological pressure and economic loss to the patient, seriously affecting the patient's health and quality of life. [0003] ...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K47/42A61K38/18A61P17/02
CPCA61K47/42A61K9/0002A61K9/19A61K38/1841A61K38/1858A61K38/1866
Inventor 单桂秋林放施琳颖周谋王玲
Owner GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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